prikaz prve stranice dokumenta Ekspresija gena xpa, acrc i tdp1 u ribe zebrice (Danio rerio) i identifikacija zebrica mutiranog gena xpa
Embargo period 2024-12-06
master's thesis
Ekspresija gena xpa, acrc i tdp1 u ribe zebrice (Danio rerio) i identifikacija zebrica mutiranog gena xpa
Zagreb: University of Zagreb, Faculty of Science, 2022. urn:nbn:hr:217:656517
Vinković, Luka
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Vinković, L. (2022). Ekspresija gena xpa, acrc i tdp1 u ribe zebrice (Danio rerio) i identifikacija zebrica mutiranog gena xpa (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:656517

Vinković, Luka. "Ekspresija gena xpa, acrc i tdp1 u ribe zebrice (Danio rerio) i identifikacija zebrica mutiranog gena xpa." Master's thesis, University of Zagreb, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:217:656517

Vinković, Luka. "Ekspresija gena xpa, acrc i tdp1 u ribe zebrice (Danio rerio) i identifikacija zebrica mutiranog gena xpa." Master's thesis, University of Zagreb, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:217:656517

Vinković, L. (2022). 'Ekspresija gena xpa, acrc i tdp1 u ribe zebrice (Danio rerio) i identifikacija zebrica mutiranog gena xpa', Master's thesis, University of Zagreb, Faculty of Science, accessed 27 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:656517

Vinković L. Ekspresija gena xpa, acrc i tdp1 u ribe zebrice (Danio rerio) i identifikacija zebrica mutiranog gena xpa [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2022 [cited 2024 November 27] Available at: https://urn.nsk.hr/urn:nbn:hr:217:656517

L. Vinković, "Ekspresija gena xpa, acrc i tdp1 u ribe zebrice (Danio rerio) i identifikacija zebrica mutiranog gena xpa", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:217:656517

Metadata
TitleEkspresija gena xpa, acrc i tdp1 u ribe zebrice (Danio rerio) i identifikacija zebrica mutiranog gena xpa
Title (english)Expression of xpa, acrc and tdp1 genes in zebrafish (Danio rerio) and identification of xpa deficient mutants
AuthorLuka Vinković
MentorMarta Popović (mentor)
MentorNenad Malenica (komentor)
Committee memberNenad Malenica (predsjednik povjerenstva)
Committee memberInga Urlić (član povjerenstva)
Committee memberIvana Ivančić Baće (član povjerenstva)
Committee memberMaja Matulić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2022-12-06, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Križne veze DNA i proteina su ireverzibilne, kovalentne veze DNA i proteina koje ograničavaju obavljanje staničnih procesa. Protein Acrc se zbog metaloproteazne domene i uloge u očuvanju genomske stabilnosti povezuje s popravkom križnih veza DNA i proteina. Još jedan mehanizam koji sudjeluje u popravku križnih veza DNA i proteina je popravak izrezivanjem nukleotida u kojem protein Xpa ima esencijalnu ulogu proteina nosača u ovom mehanizmu popravka. Tirozil DNA fosfodiesteraza 1 (Tdp1) specifična je za određenu vrstu oštećenja pa svojom hidrolitičkom aktivnošću razrješuje fosfotirozilnu vezu između topoizomeraze 1 i jednolančane DNA. Analizom ekspresije gena acrc, xpa i tdp1 lančanom reakcijom polimeraze u stvarnom vremenu u tkivima ribe zebrice (Danio rerio) uočeno je da, osim pada ekspresije ovih gena starenjem, postoje tkiva u kojima ekspresija raste, što je suprotno očekivanjima. Analizom razlike ekspresije gena između spolova uočeno je da je ekspresija u pojedinim tkivima veća kod ženki zebrice, a u pojedinim kod mužjaka pa se za određeni spol ne može tvrditi da je otporniji na križne veze DNA i proteina na temelju ekspresije gena acrc, xpa i tdp1. Uz analizu ekspresije gena cilj je bio identificirati zebrice s mutiranim genom xpa u svrhu stvaranja Xpa deficijente linije zebrica. Pronađena mutacija nije inaktivirala protein Xpa.
Abstract (english)
DNA-protein crosslinks are irreversible, covalent bonds of DNA and proteins that limit the cell performance. Acrc protein is being connected to DNA-protein crosslink repair because of its metalloprotease domain and a role in genome stability. Another mechanism involved in DNAprotein crosslink repair is nucleotide excision repair in which protein Xpa has an essential scaffolding role. Tyrosyl DNA phosphodiesterase 1 (Tdp1) is specific for a particular type of damage as it resolves the phosphotyrosyl bond between topoisomerase I and single-stranded DNA. Analysis of acrc, xpa and tdp1 gene expression with real-time polymerase chain reaction in zebrafish (Danio rerio) tissues showed that besides of the reduction in expression of these genes there are tissues that exhibit increased expression which wasn’t expected. Analysis of expression differences between zebrafish genders showed that some tissues exhibit higher expression in females while some have higher expression in males so it can’t be declared that a particular gender is more resistant to DNA-protein crosslinks based on expression of acrc, xpa and tdp1 genes. There was also a goal to identify zebrafish with mutated xpa gene with a purpose of creating a Xpa deficient zebrafish line, but the only detected modification doesn’t inactivate the protein.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:656517
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsEmbargoed access
Embargo expiration date: 2024-12-06
Terms of useLicence In copyright icon
Created on2022-12-19 12:07:20