prikaz prve stranice dokumenta Tipovi vegetativne kompatibilnosti gljive Cryphonectria parasitica i analiza ORFA regije virusa Cryphonectria hypovirus 1 kontinentalne Hrvatske
Embargo period 2024-12-19
master's thesis
Tipovi vegetativne kompatibilnosti gljive Cryphonectria parasitica i analiza ORFA regije virusa Cryphonectria hypovirus 1 kontinentalne Hrvatske
Zagreb: University of Zagreb, Faculty of Science, 2022. urn:nbn:hr:217:016104
Škegro, Ivan
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Škegro, I. (2022). Tipovi vegetativne kompatibilnosti gljive Cryphonectria parasitica i analiza ORFA regije virusa Cryphonectria hypovirus 1 kontinentalne Hrvatske (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:016104

Škegro, Ivan. "Tipovi vegetativne kompatibilnosti gljive Cryphonectria parasitica i analiza ORFA regije virusa Cryphonectria hypovirus 1 kontinentalne Hrvatske." Master's thesis, University of Zagreb, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:217:016104

Škegro, Ivan. "Tipovi vegetativne kompatibilnosti gljive Cryphonectria parasitica i analiza ORFA regije virusa Cryphonectria hypovirus 1 kontinentalne Hrvatske." Master's thesis, University of Zagreb, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:217:016104

Škegro, I. (2022). 'Tipovi vegetativne kompatibilnosti gljive Cryphonectria parasitica i analiza ORFA regije virusa Cryphonectria hypovirus 1 kontinentalne Hrvatske', Master's thesis, University of Zagreb, Faculty of Science, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:016104

Škegro I. Tipovi vegetativne kompatibilnosti gljive Cryphonectria parasitica i analiza ORFA regije virusa Cryphonectria hypovirus 1 kontinentalne Hrvatske [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2022 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:217:016104

I. Škegro, "Tipovi vegetativne kompatibilnosti gljive Cryphonectria parasitica i analiza ORFA regije virusa Cryphonectria hypovirus 1 kontinentalne Hrvatske", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:217:016104

Metadata
TitleTipovi vegetativne kompatibilnosti gljive Cryphonectria parasitica i analiza ORFA regije virusa Cryphonectria hypovirus 1 kontinentalne Hrvatske
Title (english)Vegetative compatibility types of Cryphonectria parasitica and analysis of Cryphonectria hypovirus 1 ORFA region from continental Croatia
AuthorIvan Škegro
MentorMarin Ježić (mentor)
Committee memberMarin Ježić (predsjednik povjerenstva)
Committee memberGoran Klobučar (član povjerenstva)
Committee memberNada Oršolić (član povjerenstva)
Committee memberZoran Tadić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2022-12-19, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Gljiva mješinarka Cryphonectria parasitica uzročnik je bolesti raka kore pitomog kestena (Castanea sativa), a infekcija te gljive virusom Cryphonectria hypovirus 1 (CHV1) joj smanjuje virulenciju, te tako omogućava prirodnu biološku kontrolu spomenute bolesti. Virus CHV1 je najbolje proučena vrsta hipovirusa i jedina prisutna u Europi, gdje postoji njegovih pet podtipova. Horizontalni prijenos virusa CHV1 ograničen je sustavom vegetativne (ne)kompatibilnosti gljive C. parasitica, tzv. vc-tipom gljive. U tri proučavane populacije kontinentalne Hrvatske utvrđeno je 12 različitih vc-tipova, među 54 izolata gljive C. parasitica. U populaciji Hrvatske Kostajnice najzastupljeniji vc-tipovi su EU-1 (22%), te EU-2 i EU-26 (17%), u populaciji Požege to su vctipovi EU-2 (58%) i EU-1 (19%), te konačno u populaciji Slunja to je vc-tip EU-2 (40%). Prisutnost hipovirusa dokazana je izolacijom ukupne RNA, reverznom transkripcijom, te amplifikacijom dijela genomske regije ORFA. Prevalencija virusa CHV1 na području Hrvatske Kostajnice iznosi 61%, a u Požegi 23%. Nakon sekvenciranja umnoženih fragmenata potvrđeno je da izolati virusa CHV1 pripadaju talijanskom podtipu, te su najsrodniji izolatima iz Slovenije, Italije i Švicarske.
Abstract (english)
Sac fungus Cryphonectria parasitica is a causal agent of the chestnut blight, a disease of sweet chestnut (Castanea sativa). Infection of the fungus with Cryphonectria hypovirus 1 (CHV1) reduces its virulence, acting as a natural biocontrol agent of the chestnut blight. CHV1 is the best studied species of hypoviruses and the only one present in Europe, where five subtypes of this virus have been identified. Its horizontal transmission is limited by a vegetative (in)compatibility (vctype) system of the fungus. Twelve different vc-types were identified among 54 isolates of C. parasitica from three populations of continental Croatia. The most abundant vc-type in Hrvatska Kostajnica was EU-1 (22%), followed by EU-2 and EU-26 (17%), in Požega it was EU-2 (58%), followed by EU-1 (19%). Finally, the most widespread vc-type in Slunj was EU-2 (40%). CHV1 presence was determined by isolation of total RNA, reverse transcription and amplification of a part of ORFA genomic region. The prevalence of CHV1 is 61% in Hrvatska Kostajnica and 23% in Požega. After sequencing of the amplified fragments, all virus strains have been confirmed to belong to the same, Italian subtype, and are closely related to strains from Slovenia, Italy and Switzerland.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:016104
Study programmeTitle: Ecology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra ekologije i zaštite prirode (magistar/magistra ekologije i zaštite prirode)
Type of resourceText
File originBorn digital
Access conditionsEmbargoed access
Embargo expiration date: 2024-12-19
Terms of useLicence In copyright icon
Created on2022-12-20 12:57:11