prikaz prve stranice dokumenta Distribution of auxin and PIN proteins during somatic embryogenesis of Brachypodium distachyon
Embargo period 2024-12-19
master's thesis
Distribution of auxin and PIN proteins during somatic embryogenesis of Brachypodium distachyon
Zagreb: University of Zagreb, Faculty of Science, 2022. urn:nbn:hr:217:010033
Lelas, Luka
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Lelas, L. (2022). Distribution of auxin and PIN proteins during somatic embryogenesis of Brachypodium distachyon (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:010033

Lelas, Luka. "Distribution of auxin and PIN proteins during somatic embryogenesis of Brachypodium distachyon." Master's thesis, University of Zagreb, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:217:010033

Lelas, Luka. "Distribution of auxin and PIN proteins during somatic embryogenesis of Brachypodium distachyon." Master's thesis, University of Zagreb, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:217:010033

Lelas, L. (2022). 'Distribution of auxin and PIN proteins during somatic embryogenesis of Brachypodium distachyon', Master's thesis, University of Zagreb, Faculty of Science, accessed 18 May 2024, https://urn.nsk.hr/urn:nbn:hr:217:010033

Lelas L. Distribution of auxin and PIN proteins during somatic embryogenesis of Brachypodium distachyon [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2022 [cited 2024 May 18] Available at: https://urn.nsk.hr/urn:nbn:hr:217:010033

L. Lelas, "Distribution of auxin and PIN proteins during somatic embryogenesis of Brachypodium distachyon", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:217:010033

Metadata
TitleDistribution of auxin and PIN proteins during somatic embryogenesis of Brachypodium distachyon
Title (croatian)Lokalizacija auksina i proteina PIN tijekom somatske embriogeneze vrste Brachypodium distachyon
AuthorLuka Lelas
MentorOumaya Bouchabké-Coussa (mentor)
MentorDunja Leljak-Levanić (komentor)
Committee memberDunja Leljak-Levanić (predsjednik povjerenstva)
Committee memberŽeljka Vidaković-Cifrek (član povjerenstva)
Committee memberPetra Peharec Štefanić (član povjerenstva)
Committee memberVišnja Besendorfer (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2022-12-19, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Brachypodium distachyon is a model species used for research of Poaceae. The phylogenetic link this species shares with monocots of agronomic importance makes it the ideal model for studying developmental mechanism with economic applications, like somatic embryogenesis (SE). As auxin plays a critical role in SE, tracking its presence during SE enables description of the process as a whole. For this purpose, fluorescently-tagged PIN-FORMED (PIN) auxin transporters and presence of auxin was examined using confocal microscopy. Findings confirmed PIN1a to be a major marker of SE as it is expressed in all stages of SE and revealed the complementary but mutually exclusive distribution patterns of SoPIN1 and PIN1b. Moreover, examinations of markers of auxin presence revealed how SoPIN1 and PIN1b affect its flow and thus influence SE. Additionally, with the goal of furthering understanding of the effect these select PIN auxin transporters have on SE, response of loss-of-function mutants pin1a, pin1b and their cross pin1a × pin1b to in vitro SE induction was compared using PAS/NBB staining. Results have shown no identifiable histological differences in comparison to wild-type explants, hinting at functional redundancy of the remaining PIN transporters allowing SE to occur.
Abstract (croatian)
Brachypodium distachyon je vrsta koja se koristi pri proučavanju porodice Poaceae. Njena filogenetska povezanost s jednosupnicama od agronomske važnosti čini je idealnom za proučavanje razvojnih mehanizama s ekonomskim primjenama, poput somatske embriogeneze (SE). Kako auksin ima ključnu ulogu u SE, praćenje njegove prisutnosti tijekom SE omogućava opisivanje tog procesa u cjelini. U tu svrhu, odabrani su fluorescentno označeni transporteri auksina PIN-FORMED (PIN) za prisutnost auksina te proučeni pomoću konfokalnog mikroskopa ili konfokalne mikroskopije. Rezultati su pokazali da je PIN1a važan marker SE budući da je eksprimiran tijekom svih stadija SE te da su obrasci distribucije SoPIN1 i PIN1b komplementarni i međusobno isključivi. Štoviše, proučavanje markera prisutnosti auksina pokazalo je na koji način SoPIN1 i PIN1b utječu na njegov tok i time oblikuju SE. Uz navedeno je proučen učinak odabranih PIN auksinskih transportera na proces SE, uspoređujući odgovor loss-of-function mutanta pin1a, pin1b te njihovog križanca pin1a × pin1b na indukciju SE in vitro koristeći se bojanjem PAS/NBB. Histološke razlike mutanata i eksplantanata divljeg tipa nisu utvrđene, što ukazuje kako funkcionalna redundantnost preostalih proteina PIN omogućava odvijanje SE.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:010033
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsEmbargoed access
Embargo expiration date: 2024-12-19
Terms of useLicence In copyright icon
Created on2022-12-22 08:38:59