prikaz prve stranice dokumenta NOTCH2 u meningeomima različitih gradusa: analiza metilacijskog statusa promotora gena i ekspresije proteina
Embargo period 2025-02-23
master's thesis
NOTCH2 u meningeomima različitih gradusa: analiza metilacijskog statusa promotora gena i ekspresije proteina
Zagreb: University of Zagreb, Faculty of Science, 2023. urn:nbn:hr:217:925913
Blažević, Božana
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Blažević, B. (2023). NOTCH2 u meningeomima različitih gradusa: analiza metilacijskog statusa promotora gena i ekspresije proteina (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:925913

Blažević, Božana. "NOTCH2 u meningeomima različitih gradusa: analiza metilacijskog statusa promotora gena i ekspresije proteina." Master's thesis, University of Zagreb, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:217:925913

Blažević, Božana. "NOTCH2 u meningeomima različitih gradusa: analiza metilacijskog statusa promotora gena i ekspresije proteina." Master's thesis, University of Zagreb, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:217:925913

Blažević, B. (2023). 'NOTCH2 u meningeomima različitih gradusa: analiza metilacijskog statusa promotora gena i ekspresije proteina', Master's thesis, University of Zagreb, Faculty of Science, accessed 03 May 2024, https://urn.nsk.hr/urn:nbn:hr:217:925913

Blažević B. NOTCH2 u meningeomima različitih gradusa: analiza metilacijskog statusa promotora gena i ekspresije proteina [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2023 [cited 2024 May 03] Available at: https://urn.nsk.hr/urn:nbn:hr:217:925913

B. Blažević, "NOTCH2 u meningeomima različitih gradusa: analiza metilacijskog statusa promotora gena i ekspresije proteina", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:217:925913

Metadata
TitleNOTCH2 u meningeomima različitih gradusa: analiza metilacijskog statusa promotora gena i ekspresije proteina
Title (english)NOTCH2 in different meningeoma grades: protein expression and methylation status analysis
AuthorBožana Blažević
MentorDubravka Hranilović (mentor)
MentorAnja Bukovac (komentor)
Committee memberDubravka Hranilović (predsjednik povjerenstva)
Committee memberBiljana Balen (član povjerenstva)
Committee memberMaja Matulić (član povjerenstva)
Committee memberSandra Hudina (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2023-02-23, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Meningeomi su najčešći primarni tumori središnjeg živčanog sustava klasificirani u tri histološka gradusa; benigni, atipični i anaplastični. Većinom su benignog karaktera, dok manji dio pripada višim gradusima s lošijom kliničkom slikom, povećanom stopom recidiva i smrtnosti. Signalni put Notch je neophodan u embriogenezi, ali dokazana je njegova uloga i u tumorigenezi. Protein NOTCH2 ovisno o vrsti tumora i tkiva može biti onkogen ili tumor supresor, a njegova uloga u tumorigenezi meningeoma još nije istražena. Cilj ovog diplomskog rada bio je istražiti metilaciju promotora gena i ekspresiju proteina NOTCH2 u različitim gradusima intrakranijalnih meningeoma. U ovom istraživanju, metilacija promotora gena NOTCH2 bila je značajno povezana s atipičnim oblikom meningeoma. Metilacijski status gena NOTCH2 nije utjecao na razinu ekspresije proteina. Analiza ekspresije proteina NOTCH2 pokazala je umjerenu do jaku ekspresiju na razini citoplazme i stanične membrane u većini uzoraka meningeoma te u kori velikog mozga zdrave osobe. Međutim, ekspresija na razini jezgre je detektirana samo u tumorskom tkivu te je pozitivno korelirala s intenzitetom ekspresije u citoplazmi. Rezultati ovog istraživanja upućuju na aktivnost signalnog puta Notch i uključenost proteina NOTCH2 u tumorigenezi meningeoma.
Abstract (english)
Meningiomas are the most common primary tumors of the central nervous system classified into three different histological grades; benign, atypical, and anaplastic. They are usually benign, while smaller part belongs to higher grades with worse clinical outcomes, higher recurrence, and mortality rates. The Notch signaling pathway is crucial for embryogenesis and its part in carcinogenesis has also been proven. Depending on tissue and tumor type, protein NOTCH2 can act as an oncogene or tumor suppressor, but its role in meningioma tumorigenesis still remains unknown. The aim of this master thesis was to examine gene promoter methylation and NOTCH2 protein expression in different grades of intracranial meningioma. In this study, gene promoter methylation was significantly correlated with atypical meningioma and didn't affect protein expression. Immunohistochemical analysis showed moderate to strong cytoplasmatic and cell membrane protein expression in all meningioma samples, including the cerebral cortex of a healthy person. However, expression in the nucleus was only detected in tumor tissue and it positively correlated with the intensity of cytoplasmatic expression. The results of this study indicate the activity of the Notch signalling pathway and involvement of NOTCH2 in meningioma tumorigenesis.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:925913
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsEmbargoed access
Embargo expiration date: 2025-02-23
Terms of useLicence In copyright icon
Created on2023-03-08 10:21:53