prikaz prve stranice dokumenta Raznolikost tipova vegetativne kompatibilnosti gljive Cryphonectria parasitica i genska analiza ORFA regije virusa Cryphonectria hypovirus 1 u populacijama Istre i otoka Cresa
Embargo period 2025-02-28
master's thesis
Raznolikost tipova vegetativne kompatibilnosti gljive Cryphonectria parasitica i genska analiza ORFA regije virusa Cryphonectria hypovirus 1 u populacijama Istre i otoka Cresa
Zagreb: University of Zagreb, Faculty of Science, 2023. urn:nbn:hr:217:145764
Mendaš, Ozren
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Mendaš, O. (2023). Raznolikost tipova vegetativne kompatibilnosti gljive Cryphonectria parasitica i genska analiza ORFA regije virusa Cryphonectria hypovirus 1 u populacijama Istre i otoka Cresa (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:145764

Mendaš, Ozren. "Raznolikost tipova vegetativne kompatibilnosti gljive Cryphonectria parasitica i genska analiza ORFA regije virusa Cryphonectria hypovirus 1 u populacijama Istre i otoka Cresa." Master's thesis, University of Zagreb, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:217:145764

Mendaš, Ozren. "Raznolikost tipova vegetativne kompatibilnosti gljive Cryphonectria parasitica i genska analiza ORFA regije virusa Cryphonectria hypovirus 1 u populacijama Istre i otoka Cresa." Master's thesis, University of Zagreb, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:217:145764

Mendaš, O. (2023). 'Raznolikost tipova vegetativne kompatibilnosti gljive Cryphonectria parasitica i genska analiza ORFA regije virusa Cryphonectria hypovirus 1 u populacijama Istre i otoka Cresa', Master's thesis, University of Zagreb, Faculty of Science, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:145764

Mendaš O. Raznolikost tipova vegetativne kompatibilnosti gljive Cryphonectria parasitica i genska analiza ORFA regije virusa Cryphonectria hypovirus 1 u populacijama Istre i otoka Cresa [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2023 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:217:145764

O. Mendaš, "Raznolikost tipova vegetativne kompatibilnosti gljive Cryphonectria parasitica i genska analiza ORFA regije virusa Cryphonectria hypovirus 1 u populacijama Istre i otoka Cresa", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:217:145764

Metadata
TitleRaznolikost tipova vegetativne kompatibilnosti gljive Cryphonectria parasitica i genska analiza ORFA regije virusa Cryphonectria hypovirus 1 u populacijama Istre i otoka Cresa
Title (english)Diversity of vegetative compatibility types of fungus Cryphonectria parasitica and genetic analysis of ORFA region of Cryphonectria hypovirus 1 in populations of Istria and Cres island
AuthorOzren Mendaš
MentorMarin Ježić (mentor)
Committee memberMarin Ježić (predsjednik povjerenstva)
Committee memberAnamaria Štambuk (član povjerenstva)
Committee memberBiljana Balen (član povjerenstva)
Committee memberSofia Ana Blažević (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2023-02-28, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Gljiva Cryphonectria parasitica uzrokuje bolest rak kore pitomog kestena (Castanea sativa), te je u proteklom stoljeću ugrozila šume pitomog kestena diljem Europe. Prisutnost virusa Cryphonectria hypovirus 1 (CHV1) u populacijama gljive povezana je sa smanjenjem virulentnosti i blažim tijekom bolesti raka kore kestena, tj. tijekom vremena dolazi do uspostavljanja prirodne biološke kontrole bolesti. Kako bi se razumjela sama raznolikost gljive, analizirani su tipovi vegetativne kompatibilnosti (vc) ove gljive. U ovom istraživanju utvrđena je raznolikost istraživanih populacija pomoću Shannonovog indeksa (H') koji u populaciji gljive otoka Cresa iznosi 1,52, a u populaciji Istre 1,33. U populaciji C. parasitica otoka Cresa potvrđeno je šest različitih EU tipova, dok je u populaciji Buje, Istra potvrđeno pet. Također, 50% izolata gljive populacije otoka Cresa sadrži virus CHV1, dok u populaciji Istre virus nije nađen. Računalnom analizom sekvenci virusne RNA u creskoj populaciji gljive nađeno je devet haplotipova virusa. Analizirane sekvence virusa CHV1 imaju ukupno 39 polimorfizama u 985 nukleotida, te je potvrđeno da CHV1 u populaciji Cresa pripada talijanskom podtipu.
Abstract (english)
Fungus Cryphonectria parasitica causes the chestnut blight disease in sweet chestnut (Castanea sativa) and has endangered the forests of sweet chestnut across Europe in the last century. The presence of Cryphonectria hypovirus 1 (CHV1) in fungal populations is connected to the reduction of the fungus’ virulence and a milder disease progress of chestnut blight, or in other words, a natural biological control is established over time. In order to understand the diversity of C. parasitica populations, an analysis of its vegetative compatibility (vc) types has been carried out. In this research, the diversity of the analysed populations was determined using the Shannon Diversity Index (H’), which was determined to be 1.52 in the fungal population of the island Cres, and 1.33 in the population of Buje, Istria. In the population of the C. parasitica of the island Cres six different EU-types were found, whereas in the population of Istria there were five. Moreover, 50% of the fungal isolates in the population of the island Cres were infected with the CHV1 virus, while in the population of Istria the virus was not found. By analysing CHV1 viral sequences in the population of Cres nine CHV1 haplotypes were found. Analysed sequences of the CHV1 virus have 39 polymorphisms in 985 nucleotides in total, and it has been determined that the CHV1 in the population of Cres belongs to the Italian subtype of the virus.
Keywords
Languagecroatian
URN:NBNurn:nbn:hr:217:145764
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsEmbargoed access
Embargo expiration date: 2025-02-28
Terms of useLicence In copyright icon
Created on2023-03-08 10:53:23