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master's thesis
Antitumorsko djelovanje niklozamida na stanice tumora mokraćnog mjehura
Zagreb: University of Zagreb, Faculty of Science, 2023. urn:nbn:hr:217:198625
Domjanović, Tina
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Domjanović, T. (2023). Antitumorsko djelovanje niklozamida na stanice tumora mokraćnog mjehura (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:198625

Domjanović, Tina. "Antitumorsko djelovanje niklozamida na stanice tumora mokraćnog mjehura." Master's thesis, University of Zagreb, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:217:198625

Domjanović, Tina. "Antitumorsko djelovanje niklozamida na stanice tumora mokraćnog mjehura." Master's thesis, University of Zagreb, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:217:198625

Domjanović, T. (2023). 'Antitumorsko djelovanje niklozamida na stanice tumora mokraćnog mjehura', Master's thesis, University of Zagreb, Faculty of Science, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:198625

Domjanović T. Antitumorsko djelovanje niklozamida na stanice tumora mokraćnog mjehura [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2023 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:217:198625

T. Domjanović, "Antitumorsko djelovanje niklozamida na stanice tumora mokraćnog mjehura", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:217:198625

Metadata
TitleAntitumorsko djelovanje niklozamida na stanice tumora mokraćnog mjehura
Title (english)The antitumor effect of niclosamide on bladder cancer cells
AuthorTina Domjanović
MentorJelena Korać Prlić (mentor)
MentorInga Urlić (komentor)
Committee memberInga Urlić (predsjednik povjerenstva)
Committee memberNada Oršolić (član povjerenstva)
Committee memberMaja Matulić (član povjerenstva)
Committee memberSofia Ana Blažević (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2023-02-07, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Tumor mokraćnog mjehura deseti je najčešće dijagnosticirani oblik tumora u svijetu s jako visokom stopom recidiva i niskom stopom preživljenja. Tradicionalne terapije najčešće imaju teške nuspojave, a pacijenti gotovo uvijek razviju otpornost na liječenje. Niklozamid je antiparazitski lijek koji se koristi za liječenje bolesti uzrokovanih trakavicama. Kod nekoliko tipova tumora pokazano je njegovo antitumorsko djelovanje induciranjem apoptoze i inhibicijom nekoliko signalnih puteva, kao što su WNT/CTNNB1, mTORC1, STAT3, NFκB, Notch i Hedgehog. Budući da su njegove nuspojave i utjecaj na organizam već dijelom istraženi, preusmjeravanje niklozamida prema liječenju tumora bilo bi kraće i jednostavnije od razvoja novog lijeka. Cilj mog diplomskog rada bio je ispitati mehanizme antitumorskog djelovanja niklozamida na stanice tumora mokraćnog mjehura; inducira li apoptozu i kakve učinke ima na staničnu proliferaciju. Metodom western blot analizirala sam ekspresiju proteina, a protočnom citometrijom apoptozu i stanični ciklus. Rezultati pokazuju da niklozamid inducira apoptozu i zaustavlja proliferaciju stanica tumora mokraćnog mjehura. Stoga, niklozamid u kombinaciji s postojećim lijekovima može imati potencijal za liječenje tumora mokraćnog mjehura.
Abstract (english)
Bladder cancer is the 10th most common cancer worldwide, with a very high recurrence rate and low survival rate. Traditional therapies often cause unpleasant side effects, and patients develop drug resistance. Niclosamide is an antiparasitic medication that has been used for many years to treat tapeworm infestations. In several types of tumors, niclosamide has been reported to exhibit antitumor effects by inducing apoptosis and inhibiting several signaling pathways, such as WNT/CTNNB1, mTORC1, STAT3, NFκB, Notch, and Hedgehog. As its adverse effects have been partially investigated, repurposing niclosamide as a treatment for cancer would be less time-consuming and less complicated than the development of a new drug. The aim of my master thesis was to examine the mechanisms of the antitumor activity of niclosamide on bladder cancer cells; does it induce apoptosis and what effects does it have on cell proliferation. Protein expression was analyzed by western blotting, apoptosis analysis, and cell cycle analysis using flow cytometry. These results demonstrate that niclosamide induces apoptosis and inhibits the proliferation of bladder cancer cells. Therefore, niclosamide in combination with existing drugs may have the potential to treat bladder tumors.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:198625
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2023-03-08 11:31:04