prikaz prve stranice dokumenta Uloga molekula mikroRNA miR-16-5p i miR-107 u regulaciji ekspresije gena BRCA1 i BRCA2 u seroznom raku jajnika visokog stupnja malignosti
Embargo period 2025-02-28
master's thesis
Uloga molekula mikroRNA miR-16-5p i miR-107 u regulaciji ekspresije gena BRCA1 i BRCA2 u seroznom raku jajnika visokog stupnja malignosti
Zagreb: University of Zagreb, Faculty of Science, 2023. urn:nbn:hr:217:384115
Perić, Petra
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Perić, P. (2023). Uloga molekula mikroRNA miR-16-5p i miR-107 u regulaciji ekspresije gena BRCA1 i BRCA2 u seroznom raku jajnika visokog stupnja malignosti (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:384115

Perić, Petra. "Uloga molekula mikroRNA miR-16-5p i miR-107 u regulaciji ekspresije gena BRCA1 i BRCA2 u seroznom raku jajnika visokog stupnja malignosti." Master's thesis, University of Zagreb, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:217:384115

Perić, Petra. "Uloga molekula mikroRNA miR-16-5p i miR-107 u regulaciji ekspresije gena BRCA1 i BRCA2 u seroznom raku jajnika visokog stupnja malignosti." Master's thesis, University of Zagreb, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:217:384115

Perić, P. (2023). 'Uloga molekula mikroRNA miR-16-5p i miR-107 u regulaciji ekspresije gena BRCA1 i BRCA2 u seroznom raku jajnika visokog stupnja malignosti', Master's thesis, University of Zagreb, Faculty of Science, accessed 23 December 2024, https://urn.nsk.hr/urn:nbn:hr:217:384115

Perić P. Uloga molekula mikroRNA miR-16-5p i miR-107 u regulaciji ekspresije gena BRCA1 i BRCA2 u seroznom raku jajnika visokog stupnja malignosti [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2023 [cited 2024 December 23] Available at: https://urn.nsk.hr/urn:nbn:hr:217:384115

P. Perić, "Uloga molekula mikroRNA miR-16-5p i miR-107 u regulaciji ekspresije gena BRCA1 i BRCA2 u seroznom raku jajnika visokog stupnja malignosti", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:217:384115

Metadata
TitleUloga molekula mikroRNA miR-16-5p i miR-107 u regulaciji ekspresije gena BRCA1 i BRCA2 u seroznom raku jajnika visokog stupnja malignosti
Title (english)The role of miR-16-5p and miR-107 microRNAs in the regulation of BRCA1 and BRCA2 gene expression in high-grade serous ovarian cancer
AuthorPetra Perić
MentorPetar Ozretić (mentor)
MentorNada Oršolić (komentor)
Committee memberNada Oršolić (predsjednik povjerenstva)
Committee memberJasna Lajtner (član povjerenstva)
Committee memberPetar Kružić (član povjerenstva)
Committee memberMaja Matulić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2023-02-28, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Serozni rak jajnika visokog stupnja malignosti (HGSOC) najčešći je i najsmrtonosniji podtip raka jajnika. Često je otkriven u uznapredovanoj fazi zbog izostanka jasnih i ranih simptoma, ali i nedostatka pouzdanih testova za rano otkrivanje. Glavni cilj ovog diplomskog rada je na proširenom setu uzoraka tkiva HGSOC i zdravih jajovoda odrediti ekspresiju mikroRNA hsamiR-16-5p i hsa-miR-107 te gena BRCA1 i BRCA2, za koje je utvrđeno da su njihovi ciljni geni, a čija bi poremećena ekspresija mogla biti uzrokovana poremećenom ekspresijom ovih mikroRNA. Određena je i dijagnostička vrijednost dobivenih ekspresija u razlikovanju tumorskog od zdravog tkiva. Osim toga, na staničnoj liniji karcinoma jajnika OVCAR-8 istražen je utjecaj tretmana mimicima i inhibitorima miR-16-5p i miR-107 na ekspresiju gena BRCA1 i BRCA2. Rezultati su pokazali da je ekspresija gena BRCA2 i obje miRNA značajno povećana u raku jajnika, te da postoji pozitivna korelacija između ekspresija istraživanih gena, ali i miRNA, te miR-107 i BRCA2. Ovakav rezultat ujedno ukazuje na moguću povezanost u regulaciji njihove ekspresije. Najveću dijagnostičku vrijednost pokazale su ekspresije BRCA2 i miR-107 (AUC > 0,8). Općenito, tretmani mimicima i inhibitorima su pokazali pozitivan utjecaj na ekspresiju oba gena, pri čemu je tretman inhibitorom miR-107 pokazao jednak utjecaj kao i mimik.
Abstract (english)
High-grade serous ovarian cancer (HGSOC) is the most common and deadliest subtype of ovarian cancer. It is often detected at an advanced stage due to the absence of clear and early symptoms, but also the lack of reliable tests for early detection. The main objective of this thesis was to determine the expression of microRNAs hsa-miR-16-5p and hsa-miR-107 and BRCA1/2 genes, which were found to be their target genes, on an expanded set of HGSOC tissue samples and healthy fallopian tubes, and whose impaired expression could be caused by impaired expression of these microRNAs. The diagnostic value of the obtained expressions in distinguishing tumor from healthy tissue was also determined. In addition, the influence of treatment with miR-16-5p and miR-107 mimics and inhibitors on the expression of BRCA1 and BRCA2 genes was investigated on the OVCAR-8 ovarian cancer cell line. The results showed that the expression of the BRCA2 gene and both miRNAs was significantly increased in ovarian cancer, and that there was a positive correlation between expression of investigated genes, but also miRNAs, and miR-107 and BRCA2. This result also indicates potential connection in the regulation of their expression. The highest diagnostic values were shown for the expressions of BRCA2 and miR-107 (AUC > 0.8). Generally, treatments with mimics and inhibitors showed a positive effect on the expression of both genes, but inhibitor miR-107 treatment showed the same effect as the mimic.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:384115
Study programmeTitle: Experimental Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
Type of resourceText
File originBorn digital
Access conditionsEmbargoed access
Embargo expiration date: 2025-02-28
Terms of useLicence In copyright icon
Created on2023-03-13 09:52:11