Men are at twofold to fourfold higher risk for developing head and neck cancer, which may be a result of the different lifestyle habits between men and women or a result of their different hormones. Androgens are synthesised from cholesterol and they include all hormones that have a masculinizing effect. Androgens exert their action by binding to nuclear (AR) or membrane receptors (CACNA1C, OXER1, SLC39A9). The hypothesis of this research is that androgens and androgen receptors could play a role in the development of head and neck tumors and that cholesterol depletion therapy (caused by statins) could be used in the treatment of this type of tumor. By using the quantitative polymerase chain reaction in real time, I determined the gene expression profile for nuclear and membrane androgen receptors in samples of healthy mucosa, primary tumors, metastatic lymph nodes and also in a metastatic head and neck tumor cell model (Detroit 562 cell line). By using wound healing and colony forming assays, I investigated the migratory ability of cells after the activation of androgen receptors with dihydrotestosterone and after cholesterol depletion with simvastatin. The results showed that only the nuclear androgen receptor AR could have a role in the development and metastasis of head and neck tumors and that cholesterol depletion therapy represents a potential novel therapy for this type of tumor.