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master's thesis
Učinak indol-3-maslačne kiseline na oksidacijski stres u u vinovoj lozi (Vitis vinifera L.) zaraženoj fitoplazmama
Zagreb: University of Zagreb, Faculty of Science, 2010. urn:nbn:hr:217:778022
Kozina, Andrijana
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Kozina, A. (2010). Učinak indol-3-maslačne kiseline na oksidacijski stres u u vinovoj lozi (Vitis vinifera L.) zaraženoj fitoplazmama (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:778022

Kozina, Andrijana. "Učinak indol-3-maslačne kiseline na oksidacijski stres u u vinovoj lozi (Vitis vinifera L.) zaraženoj fitoplazmama." Master's thesis, University of Zagreb, Faculty of Science, 2010. https://urn.nsk.hr/urn:nbn:hr:217:778022

Kozina, Andrijana. "Učinak indol-3-maslačne kiseline na oksidacijski stres u u vinovoj lozi (Vitis vinifera L.) zaraženoj fitoplazmama." Master's thesis, University of Zagreb, Faculty of Science, 2010. https://urn.nsk.hr/urn:nbn:hr:217:778022

Kozina, A. (2010). 'Učinak indol-3-maslačne kiseline na oksidacijski stres u u vinovoj lozi (Vitis vinifera L.) zaraženoj fitoplazmama', Master's thesis, University of Zagreb, Faculty of Science, accessed 04 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:778022

Kozina A. Učinak indol-3-maslačne kiseline na oksidacijski stres u u vinovoj lozi (Vitis vinifera L.) zaraženoj fitoplazmama [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2010 [cited 2024 November 04] Available at: https://urn.nsk.hr/urn:nbn:hr:217:778022

A. Kozina, "Učinak indol-3-maslačne kiseline na oksidacijski stres u u vinovoj lozi (Vitis vinifera L.) zaraženoj fitoplazmama", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2010. Available at: https://urn.nsk.hr/urn:nbn:hr:217:778022

Metadata
TitleUčinak indol-3-maslačne kiseline na oksidacijski stres u u vinovoj lozi (Vitis vinifera L.) zaraženoj fitoplazmama
Title (english)The effect of indol-3-butyric acid on oxidative stress in phytoplasma-infected grapevine (Vitis vinifera L.)
AuthorAndrijana Kozina
MentorMirna Ćurković Perica (mentor)
Committee memberMirna Ćurković Perica (predsjednik povjerenstva)
Committee memberMirta Tkalec (član povjerenstva)
Committee memberGordana Lacković-Venturin (član povjerenstva)
Committee memberBiljana Balen (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2010-10-12, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Fitoplazmama zaražena i zdrava vinova loza (Vitis vinifera L.) tretirana je folijarno indol-3-maslačnom kiselinom (IBA) u koncentracijama 0,5, 1,0 i 1,5 g/trsu. Metodom "ugniježdene" lančane reakcije polimerazom (PCR) određivana je prisutnost fitoplazmatskog gena za 16S rRNA prije i nakon tretmana IBA-om. Također, mjereni su pokazatelji oksidacijskog stresa kako bi utvrdili utječu li reaktivni oblici kisika na oporavak zaražene loze. Rezultati PCR-a ukazuju na prisutnost fitoplazmi u samo tri uzorka od šesnaest u kojima je prethodno potvrđena zaraza. IBA (1,0 g/trsu) je uzrokovala porast vodikovog peroksida dok se stopa lipidne peroksidacije i sadržaj ukupnih fenolnih spojeva nisu značajno promijenili nakon tretmana IBA-om. Primijećena je i povećana aktivnost peroksidaze 3 mjeseca nakon tretmana IBA-om, ali ne i neposredno nakon tretmana. Dobiveni rezultati pokazuju da IBA može djelovati na određene pokazatelje oksidacijskog stresa koji se mogu povezati s oporavkom fitoplazmama zaražene loze, no interpretacija rezultata je složena i potrebna su daljnja istraživanja.
Abstract (english)
Phytoplasma-infected and healthy grapevine (Vitis vinifera L.) was treated foliary with indole-3-butyric acid (IBA) in concentrations of 0.5, 1.0 and 1.5 g/vine. Presence of the phytoplasma gene for 16S rRNA before and after the treatment was determined by nested polymerase chain reaction (PCR). Oxidative stress markers were measured to reveal whether reactive oxygen species affect phytoplasma-recovery in grapevine. PCR results confirmed the presence of phytoplasma in only three of sixteen samples, that were previously confirmed as phytoplasma infected. IBA (1.0 g/vine) caused a rise in hydrogen peroxide, while lipid peroxidation and phenolic content was not significant changed after treatments. Peroxidase activity increased three months after the treatment with IBA, but not immediately after the treatment. Obtained results indicate that IBA may act on specific indicators of oxidative stress which could be correlated with the phytoplasma-recovery in grapevine, but the interpretation of the results is complex and further research is required.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:778022
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-05-04 07:31:57