prikaz prve stranice dokumenta Interakcije bis-benzotiazola supstituiranih amidinom s dvolančanim, trolančanim i hibridnim DNA:RNA polinukleotidima
Embargo period 2025-02-16
master's thesis
Interakcije bis-benzotiazola supstituiranih amidinom s dvolančanim, trolančanim i hibridnim DNA:RNA polinukleotidima
Zagreb: University of Zagreb, Faculty of Science, 2023. urn:nbn:hr:217:824911
Gunčević, Kristina
University of Zagreb
Faculty of Science
Department of Chemistry

Institutional repository: Repository of the Faculty of Science

Cite this document

Gunčević, K. (2023). Interakcije bis-benzotiazola supstituiranih amidinom s dvolančanim, trolančanim i hibridnim DNA:RNA polinukleotidima (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:824911

Gunčević, Kristina. "Interakcije bis-benzotiazola supstituiranih amidinom s dvolančanim, trolančanim i hibridnim DNA:RNA polinukleotidima." Master's thesis, University of Zagreb, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:217:824911

Gunčević, Kristina. "Interakcije bis-benzotiazola supstituiranih amidinom s dvolančanim, trolančanim i hibridnim DNA:RNA polinukleotidima." Master's thesis, University of Zagreb, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:217:824911

Gunčević, K. (2023). 'Interakcije bis-benzotiazola supstituiranih amidinom s dvolančanim, trolančanim i hibridnim DNA:RNA polinukleotidima', Master's thesis, University of Zagreb, Faculty of Science, accessed 26 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:824911

Gunčević K. Interakcije bis-benzotiazola supstituiranih amidinom s dvolančanim, trolančanim i hibridnim DNA:RNA polinukleotidima [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2023 [cited 2024 November 26] Available at: https://urn.nsk.hr/urn:nbn:hr:217:824911

K. Gunčević, "Interakcije bis-benzotiazola supstituiranih amidinom s dvolančanim, trolančanim i hibridnim DNA:RNA polinukleotidima", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:217:824911

Metadata
TitleInterakcije bis-benzotiazola supstituiranih amidinom s dvolančanim, trolančanim i hibridnim DNA:RNA polinukleotidima
Title (english)Interactions of amidino-substituted bis-benzothiazoles with double-stranded, triple-stranded and hybrid DNA:RNA polynucleotides
AuthorKristina Gunčević
MentorLidija-Marija Tumir (mentor)
Committee memberMorana Dulić (predsjednik povjerenstva)
Committee memberSanda Rončević (član povjerenstva)
Committee memberMirta Rubčić (član povjerenstva)
Committee memberMarko Močibob (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Chemistry)
Zagreb
Defense date and country2023-02-16, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
Derivati benzotiazola s amidinskim funkcionalnim skupinama pokazuju značajnu biološku aktivnost. Četiri novosintetizirana derivata bis-benzotiazola supstituirana amidinom spektroskopski su okarakterizirana te im je određena temperaturna stabilnost. Ispitane su interakcije derivata bis-benzotiazola supstituiranih amidinom s DNA, RNA i hibridne DNA:RNA polinukleotide. Kompeticijskom dijalizom probrani su polinukleotidi prema kojima spojevi pokazuju najveći afinitet i selektivnost, te su oni korišteni u daljnjem istraživanju. Eksperimenti temperature mekšanja pokazali su da benzotiazoli stabiliziraju dvolančane, odnosno trolančane uzvojnice polinukleotida. Dodatak polinukleotida ispitivanim spojevima uzrokovao je gašenje fluorescencije te su, na temelju fluorescencijskih titracija, izračunate ili procijenjene konstante asocijacije. Spektri titracija dobiveni metodom cirkularnog dikroizma ukazuju na mješovite načine vezanja; interkalaciju te vezanje u mali utor i agregiranje spoja duž fosfatne okosnice. Dobiveni rezultati ukazuju na selektivnost spojeva prema određenim sekvencama i strukturama nukleinskih kiselina.
Abstract (english)
Amidino-substituted benzothiazole derivates showed significant biological activity. Four newly synthesized derivates of amidino-substituted bis-benzothiazoles were spectroscopically characterized and their temperature stability was determined. Interactions of amidinosubstituted bis-benzothiazoles with DNA, RNA and hybrid DNA:RNA polynucleotides were examined. Interaction of an array of polynucleotides with four benzothiazole derivates was screened by competition dialysis. According to the screening results (affinities and selectivity) polynucleotides were selected for detailed characterization. The thermal melting experiment indicated that benzothiazoles stabilised double-stranded and triple-stranded polynucleotide helixes. The addition of polynucleotide to the compound solution caused fluorescence quenching. Association constants were calculated or estimated based on fluorimetric titrations. Circular dichroism spectroscopy indicated mixed binding modes: intercalation, minor groove binding and aggregation along the phosphate backbone. The obtained results indicated benzothiazoles selectivity for specific polynucleotide sequences and structures.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:824911
ProjectNumber: IP-2018-01-4694
Title: Molekularno prepoznavanje DNA:RNA hibridnih i višelančanih struktura u bioanalitičkim i in vitro sustavima
Acronym: DNARNAHyB-MolBio
Leader: Marijana Radić Stojković
Jurisdiction: Croatia
Funder: HRZZ
Funding stream: IP
Study programmeTitle: Graduate university studies in chemistry; specializations in: Research programme
Course: Research programme
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
Type of resourceText
File originBorn digital
Access conditionsEmbargoed access
Embargo expiration date: 2025-02-16
Terms of useLicence In copyright icon
Created on2023-03-29 11:25:09