prikaz prve stranice dokumenta ULOGA PROTEINA IQGC U REGULACIJI STANIČNE ADHEZIJE U PROTISTU DICTYOSTELIUM DISCOIDEUM
Embargo period 2025-03-24
doctoral thesis
ULOGA PROTEINA IQGC U REGULACIJI STANIČNE ADHEZIJE U PROTISTU DICTYOSTELIUM DISCOIDEUM
Zagreb: University of Zagreb, Faculty of Science, 2023. urn:nbn:hr:217:565685
Mijanović, Lucija
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Mijanović, L. (2023). ULOGA PROTEINA IQGC U REGULACIJI STANIČNE ADHEZIJE U PROTISTU DICTYOSTELIUM DISCOIDEUM (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:565685

Mijanović, Lucija. "ULOGA PROTEINA IQGC U REGULACIJI STANIČNE ADHEZIJE U PROTISTU DICTYOSTELIUM DISCOIDEUM." Doctoral thesis, University of Zagreb, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:217:565685

Mijanović, Lucija. "ULOGA PROTEINA IQGC U REGULACIJI STANIČNE ADHEZIJE U PROTISTU DICTYOSTELIUM DISCOIDEUM." Doctoral thesis, University of Zagreb, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:217:565685

Mijanović, L. (2023). 'ULOGA PROTEINA IQGC U REGULACIJI STANIČNE ADHEZIJE U PROTISTU DICTYOSTELIUM DISCOIDEUM', Doctoral thesis, University of Zagreb, Faculty of Science, accessed 25 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:565685

Mijanović L. ULOGA PROTEINA IQGC U REGULACIJI STANIČNE ADHEZIJE U PROTISTU DICTYOSTELIUM DISCOIDEUM [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Science; 2023 [cited 2024 November 25] Available at: https://urn.nsk.hr/urn:nbn:hr:217:565685

L. Mijanović, "ULOGA PROTEINA IQGC U REGULACIJI STANIČNE ADHEZIJE U PROTISTU DICTYOSTELIUM DISCOIDEUM", Doctoral thesis, University of Zagreb, Faculty of Science, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:217:565685

Metadata
TitleULOGA PROTEINA IQGC U REGULACIJI STANIČNE ADHEZIJE U PROTISTU DICTYOSTELIUM DISCOIDEUM
Title (english)THE ROLE OF IQGC PROTEIN IN THE REGULATION OF ADHESION IN DICTYOSTELIUM DISCOIDEUM PROTIST
AuthorLucija Mijanović
MentorIgor Weber (mentor)
Committee memberBiljana Balen (predsjednik povjerenstva)
Committee memberInga Urlić (član povjerenstva)
Committee memberVedrana Filić Mileta (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2023-03-24, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Universal decimal classification
(UDC)		577 - Biochemistry. Molecular biology. Biophysics
Abstract
Protein IqgC iz protista Dictyostelium discoideum dijeli svojstva proteina iz skupina IQGAP i RasGAP te regulira endocitozu deaktivacijom male GTPaze RasG. Cilj ovog rada je karakterizacija dodatne uloge proteina IqgC u regulaciji stanične adhezije. Testovima adhezije pokazala sam da stanice mutanta iqgCimaju defekt u pričvršćivanju za podlogu. Ekspresijom skraćenih varijanti proteina IqgC u stanicama iqgCutvrdila sam da je za normalnu adheziju nužna prisutnost obje domene proteina, domene RGCt i domene GAP. Korištenjem mikroskopije TIRF ustanovila sam da je protein IqgC lokaliziran u fokalnim adhezijskim strukturama, gdje ga navodi domena RGCt. Biokemijskim pokusima i metodom BiFC ustanovila sam da protein IqgC veže malu GTPazu RapA. Analizom kretanja stanica otkrila sam anomalije u nasumičnom kretanju i kemotaksiji stanica mutanta iqgC- , vjerojatno posredovane deregulacijom aktivnosti GTPaza RasG i RapA. Analizom lokalizacije i aktivnosti proteina IqgC u staničnim sojevima deficijentnim za razne proteine regulatore adhezije identificirala sam signalni put reguliran proteinom RapA unutar kojeg protein IqgC djeluje kao pozitivni regulator adhezije.
Abstract (english)
IqgC protein from the protist Dictyostelium discoideum shares the properties of IQGAP and RasGAP proteins and regulates endocytosis by deactivating small GTPase RasG. The goal of this work is an investigation of an additional role of IqgC in the regulation of adhesion. Using adhesion assays, I showed that mutant iqgCcells attach poorly to the substratum. Rescue experiments with truncated variants of IqgC showed that both RGCt and GAP domains are necessary for normal adhesion. Using TIRF microscopy, I established that IqgC is localized in focal adhesion structures and that the RGCt domain is responsible for its proper localization. Biochemistry and BiFC experiments identified an interaction between IqgC and RapA. Migration assays detected anomalies in random motility and chemotaxis of iqgCcells, likely mediated by deregulation of RasG and RapA signaling. By testing the localization and activity of the IqgC protein in cell strains deficient for various adhesion regulators, I identified a signaling pathway regulated by RapA where IqgC acts as a positive regulator of cell adhesion.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:565685
Promotion2023
Study programmeTitle: Biology
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
Type of resourceText
File originBorn digital
Access conditionsEmbargoed access
Embargo expiration date: 2025-03-24
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Created on2023-04-03 09:25:54