prikaz prve stranice dokumenta Isolation and characterisation of bovine muscle stem cell progenitors
  Download
PDF 1.89 MB
master's thesis
Isolation and characterisation of bovine muscle stem cell progenitors
Zagreb: University of Zagreb, Faculty of Science, 2023. urn:nbn:hr:217:984210
Čačković, Josip
University of Zagreb
Faculty of Science
Department of Chemistry

Institutional repository: Repository of the Faculty of Science

Cite this document

Čačković, J. (2023). Isolation and characterisation of bovine muscle stem cell progenitors (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:984210

Čačković, Josip. "Isolation and characterisation of bovine muscle stem cell progenitors." Master's thesis, University of Zagreb, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:217:984210

Čačković, Josip. "Isolation and characterisation of bovine muscle stem cell progenitors." Master's thesis, University of Zagreb, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:217:984210

Čačković, J. (2023). 'Isolation and characterisation of bovine muscle stem cell progenitors', Master's thesis, University of Zagreb, Faculty of Science, accessed 03 July 2024, https://urn.nsk.hr/urn:nbn:hr:217:984210

Čačković J. Isolation and characterisation of bovine muscle stem cell progenitors [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2023 [cited 2024 July 03] Available at: https://urn.nsk.hr/urn:nbn:hr:217:984210

J. Čačković, "Isolation and characterisation of bovine muscle stem cell progenitors", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:217:984210

Metadata
TitleIsolation and characterisation of bovine muscle stem cell progenitors
Title (croatian)Izolacija i karakterizacija matičnih stanica prekursora mišićnih stanica iz goveda
AuthorJosip Čačković
MentorPetra Kluger (mentor)
Committee memberMarko Močibob (predsjednik povjerenstva)
Committee memberIvan Kodrin (član povjerenstva)
Committee memberIva Juranović Cindrić (član povjerenstva)
Committee memberJasmina Rokov Plavec (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Chemistry)
Zagreb
Defense date and country2023-04-03, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
This thesis investigates the technical feasibility of bovine muscle progenitor stem cells as a source for in vitro meat cultivation. The cells were isolated using enzymatic digestion. Bovine muscle progenitor stem cells were identified using the paired box protein, Pax7 using immunocytochemical assays and fluorescence microscopy. The cells were treated with SB203580, a common inhibitor for the p38 mitogen activated protein kinase pathway to examine its influence on the cell’s proliferation and differentiation capabilities. However, it didn’t yield the expected outcome. Collagen type 1, gelatine and laminin were used as coatings and compared to see their influence on the selectivity of the target cell type, of which laminin was the most promising. Lastly, cells were sorted via fluorescence activated cell sorting using CD29, CD56 as positive, and CD31 and CD45 as negative cell surface markers, which proved to be an auspicious method for the purification of the cell culture.
Abstract (croatian)
Ova disertacija istražuje tehničku izvedivost korištenja matičnih stanica prekursora mišićnih stanica iz goveda za uzgoj mesa in vitro. Stanice su izolirane primjenom enzimatske digestije. Matične stanice prekursori mišićnih stanica iz goveda identificirane su pomoću transkripcijskog faktora Pax7, uporabom imunocitokemijskih testova i fluorescentne mikroskopije. Stanice su tretirane SB203580, inhibitorom metaboličkog puta p38 mitogen-aktivirane proteinske kinaze, kako bi se ispitao njezin utjecaj na sposobnosti proliferacije i diferencijacije stanica. Međutim, korištenje istog nije dalo očekivani rezultat. Stanično posuđe tretirano je raznovrsnim premazima: kolagen tip 1, želatina i laminin, kako bi se promatrao njihov utjecaj na proliferaciju i selektivnost prema stanicama od interesa. Laminin se pokazao kao najobećavajući. Konačno, stanice su sortirane putem fluorescentno aktiviranog sortiranja stanica primjenom CD29, CD56 kao pozitivnih i CD31 i CD45 kao negativnih površinskih staničnih markera, što se pokazalo izvrsnom metodom za pročišćavanje kulture stanica.
Keywords
Keywords (english)
Languageenglish
URN:NBNurn:nbn:hr:217:984210
Study programmeTitle: Graduate university studies in chemistry; specializations in: Research programme
Course: Research programme
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2023-04-04 13:01:59