prikaz prve stranice dokumenta Izražaj glijalnog fibrilarnog kiselog proteina (GFAP) u mozgu odraslih štakora nakon perinatalne hipoksije
Embargo period 2025-01-20
master's thesis
Izražaj glijalnog fibrilarnog kiselog proteina (GFAP) u mozgu odraslih štakora nakon perinatalne hipoksije
Zagreb: University of Zagreb, Faculty of Science, 2023. urn:nbn:hr:217:571659
Radošević, Marta
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Radošević, M. (2023). Izražaj glijalnog fibrilarnog kiselog proteina (GFAP) u mozgu odraslih štakora nakon perinatalne hipoksije (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:571659

Radošević, Marta. "Izražaj glijalnog fibrilarnog kiselog proteina (GFAP) u mozgu odraslih štakora nakon perinatalne hipoksije." Master's thesis, University of Zagreb, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:217:571659

Radošević, Marta. "Izražaj glijalnog fibrilarnog kiselog proteina (GFAP) u mozgu odraslih štakora nakon perinatalne hipoksije." Master's thesis, University of Zagreb, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:217:571659

Radošević, M. (2023). 'Izražaj glijalnog fibrilarnog kiselog proteina (GFAP) u mozgu odraslih štakora nakon perinatalne hipoksije', Master's thesis, University of Zagreb, Faculty of Science, accessed 04 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:571659

Radošević M. Izražaj glijalnog fibrilarnog kiselog proteina (GFAP) u mozgu odraslih štakora nakon perinatalne hipoksije [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2023 [cited 2024 November 04] Available at: https://urn.nsk.hr/urn:nbn:hr:217:571659

M. Radošević, "Izražaj glijalnog fibrilarnog kiselog proteina (GFAP) u mozgu odraslih štakora nakon perinatalne hipoksije", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:217:571659

Metadata
TitleIzražaj glijalnog fibrilarnog kiselog proteina (GFAP) u mozgu odraslih štakora nakon perinatalne hipoksije
Title (english)Expression of the glial fibrillary acidic protein (GFAP) in the adult rat brain following perinatal hypoxia
AuthorMarta Radošević
MentorNataša Jovanov Milošević (mentor)
MentorDuje Lisičić (komentor)
Committee memberDuje Lisičić (predsjednik povjerenstva)
Committee memberMarija Ivković (član povjerenstva)
Committee memberSofia Ana Blažević (član povjerenstva)
Committee memberDomagoj Đikić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2023-01-20, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Cilj ovog pilot istraživanja je utvrditi učinak kratkotrajne perinatalne hipoksijske ozljede u odraslih štakora na razinu izražaja GFAP-a. Korišteno je osam ženki Wistar Han (RccHan:WIST) štakora dobi P(105) – četiri kontrolne i četiri perinatalno podvrgnute kratkotrajnoj hipoksiji. Histološki preparati bili su podvrgnuti imunofluorescentnom bojenju korištenjem anti-GFAP protutijela i flourescentnom Nissl bojenju. Kvalitativna analiza 20 presjeka nije pokazala promjene u citoarhitektonici hipokampusa, dok je uočen viši intenzitet izražaja GFAP+ astrocita nakon kratkotrajne perinatalne hipoksije. Kvantitativna analiza 20 presjeka pokazala je tendenciju k povećanju gustoće astrociti/mm2 u polju CA1 u hipoksijskoj skupini štakora. Gustoća astrociti/mm2 u polju CA2 ne pokazuje razlike između hipoksijske i kontrolne skupine. Izazvana kratkotrajna perinatalna hipoksija bila je blaga i uzrokovala je suptilne promjene koje nisu detektirane na ovom broju uzoraka. Potencijalno daljnje istraživanje povećanjem broja životinja u eksperimentu te samim time i broja uzoraka, moglo bi pokazati je li promjena morfologije astrocita ili je povećanje izražaja GFAP+ astrocita, razlog kvalitativnog utiska da je GFAP pozitivnih astrocita više u hipokampusu perinatalno hipoksijsko ozlijeđenih štakora.
Abstract (english)
The aim of this pilot study was to research the effects of short-term perinatal hypoxia on the expression of GFAP. For this purpose, eight female Wistar Han (RccHan:WIST) rats age P(105) – four controls and four perinatally treated with short-term hypoxia, were used. Samples were double stained using anti-GFAP antibody and Nissl stain. Qualitative analysis of 20 slices didn't indicate any change in the cytoarchitecture of the hippocampus, while there was a slight increase detected in the GFAP+ astrocyte signal after hypoxia. Quantitative analysis of 20 slices shows a tendency towards an increase in density of astrocyte/mm2 in the CA1 field of the hypoxic rat group. Density of astrocyte/mm2 in the CA2 field didn't show any difference between hypoxic and control group. The perinatal hypoxic injury in this study was short-term and mild, and it caused subtle changes which could not be registered on this small sample size. Future experiments, with a bigger sample size, might show if the qualitative phenomen of more GFAP positive astrocytes in the hippocampus of perinatal hypoxically injured rats, is due to change in astrocyte morphology or due to an increase in GFAP expression in astrocytes.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:571659
ProjectNumber: IP-2019-04-3182
Title: Moždani izvanstanični matriks u razvitku i perinatalnoj hipoksiji
Title: Brain extracellular matrix in development and in perinatal hypoxia
Acronym: BRAINECM
Leader: Nataša Jovanov Milošević
Jurisdiction: Croatia
Funder: HRZZ
Funding stream: IP
ProjectNumber: GA KK.01.1.1.01.0007
Title: Eksperimentalna i klinička istraživanja hipoksijsko-ishemijskog oštećenja mozga u perinatalnoj i odrasloj dobi, ZCI-Neuro
Leader: Miloš Judaš
Jurisdiction: Croatia
Funder: ERDF
Study programmeTitle: Experimental Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
Type of resourceText
File originBorn digital
Access conditionsEmbargoed access
Embargo expiration date: 2025-01-20
Terms of useLicence In copyright icon
Public noteSveučilišna potpora br. 10106-22-3116, voditeljica projekta prof. dr. sc. Nataša Jovanov- Milošević
Created on2023-04-05 10:08:23