prikaz prve stranice dokumenta Raznovrsnost alela i haplotipskih veza gena HLA-B*35 u populaciji Hrvatske
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master's thesis
Raznovrsnost alela i haplotipskih veza gena HLA-B*35 u populaciji Hrvatske
Zagreb: University of Zagreb, Faculty of Science, 2010. urn:nbn:hr:217:853936
Sušec, Mirta
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Sušec, M. (2010). Raznovrsnost alela i haplotipskih veza gena HLA-B*35 u populaciji Hrvatske (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:853936

Sušec, Mirta. "Raznovrsnost alela i haplotipskih veza gena HLA-B*35 u populaciji Hrvatske." Master's thesis, University of Zagreb, Faculty of Science, 2010. https://urn.nsk.hr/urn:nbn:hr:217:853936

Sušec, Mirta. "Raznovrsnost alela i haplotipskih veza gena HLA-B*35 u populaciji Hrvatske." Master's thesis, University of Zagreb, Faculty of Science, 2010. https://urn.nsk.hr/urn:nbn:hr:217:853936

Sušec, M. (2010). 'Raznovrsnost alela i haplotipskih veza gena HLA-B*35 u populaciji Hrvatske', Master's thesis, University of Zagreb, Faculty of Science, accessed 23 December 2024, https://urn.nsk.hr/urn:nbn:hr:217:853936

Sušec M. Raznovrsnost alela i haplotipskih veza gena HLA-B*35 u populaciji Hrvatske [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2010 [cited 2024 December 23] Available at: https://urn.nsk.hr/urn:nbn:hr:217:853936

M. Sušec, "Raznovrsnost alela i haplotipskih veza gena HLA-B*35 u populaciji Hrvatske", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2010. Available at: https://urn.nsk.hr/urn:nbn:hr:217:853936

Metadata
TitleRaznovrsnost alela i haplotipskih veza gena HLA-B*35 u populaciji Hrvatske
Title (english)DIVERSITY OF ALLELES AND HAPLOTYPE CONNECTIONS OF GENE HLA-B*35 IN CROATIAN POPULATION
AuthorMirta Sušec
MentorZorana Grubić (mentor)
Committee memberZorana Grubić (predsjednik povjerenstva)
Committee memberNada Oršolić (član povjerenstva)
Committee memberMladen Kučinić (član povjerenstva)
Committee memberGordana Lacković-Venturin (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2010-09-27, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
U ovom radu analizirali smo skupinu od 1020 nesrodnih ispitanika pozitivnih za gen HLA-B*35 i članove obitelji gdje je jedan od roditelja nositelj gena HLA-B*35 (N=50). Svi ispitanicima izolirana je DNA iz periferne krvi i testirani su za lokuse HLA-A, -B i –DRB1 metodom Luminex (srednje razlučivanje), a dio uzoraka (N=50) i metodom PCR-SSP za gen HLA-B*35 (visoko razlučivanje). Od 5 različitih alela gena HLA-B*35 uočenih u Hrvatskoj, najčešći j e bio HLA-B*35:01, zatim B*35:03, B*35:02 te B*35:08, dok je B*35:27 uočen samo jednom. Najčešća specifičnost na lokusu HLA-A unutar skupina HLA-B*35:01 i B*35:03 bila je HLA-A*02. Na lokusu HLA-DRB1 specifičnost HLA-DRB1*11 bila je najčešća među ispitanicima s alelima HLA-B*35:02 , B*35:03 i B*35:08, dok je u skupini HLA-B*35:01 ispitanika najčešća bila specifičnost HLADRB1* 01. Analiza haplotipskih veza HLA-A/B*35/DRB1 pokazala je da su slijedeće kombinacije bile najčešće: HLA-A*03/B*35:01/DRB1*01, HLA-A*02/B*35:02/DRB1*11, HLA-A*02/B*35:03/DRB1*11 i HLA-A*24/B*35:08/DRB1*11.
Abstract (english)
In this study we analyzed group of 1020 HLA-B*35 positive unrelated individuals and families with one of the parents HLA-B*35 positive (N=50). DNA from all subjects was isolated from peripheral blood and tested for HLA-A, -B, and –DRB1 by Luminex (medium resolution), while 50 samples were also typed by PCR-SSP for HLA-B*35 (high resolution). The most frequent allele of gene HLA-B*35 was HLAB* 35:01, followed by B*35:03, B*35:02 and B*35:08. We also observed one individual with B*35:27. The most common specificity at HLA-A locus among individuals positive for HLA-B*35:01 and B*35:03 was HLA-A*02. Among individuals positive for HLA-B*35:02, B*35:03 and B*35:08 the most present was HLA-DRB1*11 at HLADRB1 locus, while among B*35:01 individuals the most frequent was DRB1*01 specificity. Analysis of HLA-A/B*35/DRB1 haplotypic associations showed that the following combinations were the most common: HLA-A*03/B*35:01/DRB1*01, HLAA* 02/B*35:02/DRB1*11,HLA-A*02/B*35:03/DRB1*11 and HLA-A*24/B*35:08/DRB1*11.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:853936
Study programmeTitle: Experimental Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-05-04 07:52:30