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master's thesis
Biološka i molekularna karakterizacija izolata virusa pjegavosti i venuća rajčice
Zagreb: University of Zagreb, Faculty of Science, 2023. urn:nbn:hr:217:622243
Pul, Patrik
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Pul, P. (2023). Biološka i molekularna karakterizacija izolata virusa pjegavosti i venuća rajčice (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:622243

Pul, Patrik. "Biološka i molekularna karakterizacija izolata virusa pjegavosti i venuća rajčice." Master's thesis, University of Zagreb, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:217:622243

Pul, Patrik. "Biološka i molekularna karakterizacija izolata virusa pjegavosti i venuća rajčice." Master's thesis, University of Zagreb, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:217:622243

Pul, P. (2023). 'Biološka i molekularna karakterizacija izolata virusa pjegavosti i venuća rajčice', Master's thesis, University of Zagreb, Faculty of Science, accessed 13 May 2024, https://urn.nsk.hr/urn:nbn:hr:217:622243

Pul P. Biološka i molekularna karakterizacija izolata virusa pjegavosti i venuća rajčice [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2023 [cited 2024 May 13] Available at: https://urn.nsk.hr/urn:nbn:hr:217:622243

P. Pul, "Biološka i molekularna karakterizacija izolata virusa pjegavosti i venuća rajčice", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:217:622243

Metadata
TitleBiološka i molekularna karakterizacija izolata virusa pjegavosti i venuća rajčice
Title (english)Biological and molecular characterization of tomato spotted wilt virus isolates
AuthorPatrik Pul
MentorDijana Škorić (mentor)
Committee memberDijana Škorić (predsjednik povjerenstva)
Committee memberPetra Korać (član povjerenstva)
Committee memberTomislav Ivanković (član povjerenstva)
Committee memberMartina Šeruga Musić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2023-05-08, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Virus pjegavosti i venuća rajčice (Tomato spotted wilt virus, TSWV) identificiran je prvi put 1930. godine u Australiji kao uzročnik bolesti pjegavosti i venuća rajčice (Solanum lycopersicum L.). Tijekom 2021. i 2022. godine su u Hrvatskoj zabilježeni simptomi TSWV-a na kultivarima rajčica koji se reklamiraju kao rezistentni od strane proizvođača sjemena. Cilj ovog rada bio je potvrditi TSWV kao uzročnika infekcije različitih biljaka s terena i odrediti biološke i molekularne karakteristike virusnih izolata. Infekcija TSWV-om potvrđena je kod tri uzorka metodom reverzne transkripcije i lančane reakcije polimerazom (RT-PCR). Filogenetskim analizama amplikona pokazano je da se radi o izolatima virusa koji potječu iz regionalne virusne populacije. Analizom aminokiselinskog slijeda za nestrukturni virusni protein NSm kod jednog izolata pokazano je da se radi o varijanti virusa koja ne sadrži mutacije povezane s probijanjem rezistencije kod rajčice. Biološki test kojim se nastojalo istražiti biološke značajke (krug domaćina, simptomatologija i probijanje rezistencije kod rajčice) izolata TSWV-a nije bio uspješan zbog termolabilnosti virusa i gubitka infektivnosti biljnog materijala uslijed neadekvatnih uvjeta pohrane.
Abstract (english)
Tomato spotted wilt virus (TSWV) was first identified in 1930. in Australia as the causative agent of the spotted wilt disease of tomato (Solanum lycopersicum L.). In Croatia, during 2021. and 2022. symptoms of TSWV were observed on tomato cultivars marketed as resistant by seed suppliers. The aim of this study was to confirm TSWV as the agent infecting various field plants and to determine the biological and molecular characteristics of virus isolates. Infection with TSWV was confirmed in three isolates by reverse transcription and polymerase chain reaction (RT-PCR). Phylogenetic analyses of amplicons have shon that the virus isolates originated from the regional viral population. The nonstructural protein NSm amino acid sequence analysis from one isolate revealed the absence of mutations linked to the resistance-breaking phenotype in tomato. The bioassays conducted to examine biological characteristics (host range, symptomatology, resistance-breaking phenotype in tomato) of TSWV isolates was unsuccessful due to thermal inactivation of the virus and the loss of infectivity in inadequately stored plant material.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:622243
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2023-11-08
Terms of useLicence In copyright icon
Created on2023-05-12 09:45:13