prikaz prve stranice dokumenta Proteomics-based approaches for validation of antibodies against OTR and V1aR
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master's thesis
Proteomics-based approaches for validation of antibodies against OTR and V1aR
Zagreb: University of Zagreb, Faculty of Science, 2023. urn:nbn:hr:217:185973
Kuridža, Bojan
University of Zagreb
Faculty of Science
Department of Chemistry

Institutional repository: Repository of the Faculty of Science

Cite this document

Kuridža, B. (2023). Proteomics-based approaches for validation of antibodies against OTR and V1aR (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:185973

Kuridža, Bojan. "Proteomics-based approaches for validation of antibodies against OTR and V1aR." Master's thesis, University of Zagreb, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:217:185973

Kuridža, Bojan. "Proteomics-based approaches for validation of antibodies against OTR and V1aR." Master's thesis, University of Zagreb, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:217:185973

Kuridža, B. (2023). 'Proteomics-based approaches for validation of antibodies against OTR and V1aR', Master's thesis, University of Zagreb, Faculty of Science, accessed 26 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:185973

Kuridža B. Proteomics-based approaches for validation of antibodies against OTR and V1aR [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2023 [cited 2024 November 26] Available at: https://urn.nsk.hr/urn:nbn:hr:217:185973

B. Kuridža, "Proteomics-based approaches for validation of antibodies against OTR and V1aR", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:217:185973

Metadata
TitleProteomics-based approaches for validation of antibodies against OTR and V1aR
Title (croatian)Validacija antitijela za OTR i V1aR pristupima temeljenima na proteomici
AuthorBojan Kuridža
MentorMarkus Muttenthaler (mentor)
Committee memberMarko Močibob (predsjednik povjerenstva)
Committee memberIvan Kodrin (član povjerenstva)
Committee memberIva Juranović Cindrić (član povjerenstva)
Committee memberĐani Škalamera (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Chemistry)
Zagreb
Defense date and country2023-06-06, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
The oxytocin receptor (OTR) and the vasopressin 1a receptor (V1aR) are two G protein-coupled receptors involved in mediating various physiological processes and social and sexual behaviors. Producing specific antibodies to study these receptors is challenging due to their sequence homology and structure similarity, along with two additional closely related vasopressin receptors. Thus, this thesis aimed to validate ten antibodies to OTR and seven antibodies to V1aR produced against distinctive receptor peptide epitopes, using western blot and immunoprecipitation followed by mass spectrometry. The validation process pointed towards one V1aR antibody (1G1), which showed promising results in both western blot and immunoprecipitation experiments, and needs to be further tested. The best OTR antibody identified in immunoprecipitation experiments (1B2) did not perform well in western blots.
Abstract (croatian)
Oksitocinski receptor (OTR) i vazopresinski 1a receptor (V1aR) dva su receptora spregnuta sa G-proteinom uključena u posredovanje raznih fizioloških procesa te društvenog i seksualnog ponašanja. Proizvodnja specifičnih antitijela za proučavanje ovih receptora predstavlja izazov zbog sličnosti njihovog slijeda i strukture, kao i sličnosti dva dodatna blisko povezana vazopresinska receptora. Stoga, cilj je ovog rada bio validirati deset antitijela za OTR i sedam antitijela za V1aR proizvedenih prema karakterističnim peptidnim epitopima receptora, koristeći western blot i imunoprecipitaciju povezanu s masenom spektrometrijom. Jedno antitijelo za V1aR (1G1) u procesu validacije pokazalo je obećavajuće rezultate u eksperimentima western blota i imunoprecipitacije. Najbolje od OTR antitijela identificiranih u eksperimentima imunoprecipitacije (1B2) nije se pokazalo uspješnim u western blotovima.
Keywords
Keywords (english)
Languageenglish
URN:NBNurn:nbn:hr:217:185973
Study programmeTitle: Graduate university studies in chemistry; specializations in: Research programme
Course: Research programme
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2023-06-09 10:18:20