prikaz prve stranice dokumenta Biokemijska karakterizacija proteina RRAS2 iz spužve Eunapius subterraneus ; Istraživačka nastava kemije
Embargo period 2025-02-23
master's thesis
Biokemijska karakterizacija proteina RRAS2 iz spužve Eunapius subterraneus ; Istraživačka nastava kemije
Zagreb: University of Zagreb, Faculty of Science, 2023. urn:nbn:hr:217:021274
Lazarin, Leonarda
University of Zagreb
Faculty of Science
Department of Biology
University of Zagreb
Faculty of Science
Department of Chemistry

Institutional repository: Repository of the Faculty of Science

Cite this document

Lazarin, L. (2023). Biokemijska karakterizacija proteina RRAS2 iz spužve Eunapius subterraneus ; Istraživačka nastava kemije (Master's thesis). Zagreb: University of Zagreb, Faculty of Science; Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:021274

Lazarin, Leonarda. "Biokemijska karakterizacija proteina RRAS2 iz spužve Eunapius subterraneus ; Istraživačka nastava kemije." Master's thesis, University of Zagreb, Faculty of Science; University of Zagreb, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:217:021274

Lazarin, Leonarda. "Biokemijska karakterizacija proteina RRAS2 iz spužve Eunapius subterraneus ; Istraživačka nastava kemije." Master's thesis, University of Zagreb, Faculty of Science; University of Zagreb, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:217:021274

Lazarin, L. (2023). 'Biokemijska karakterizacija proteina RRAS2 iz spužve Eunapius subterraneus ; Istraživačka nastava kemije', Master's thesis, University of Zagreb, Faculty of Science; University of Zagreb, Faculty of Science, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:021274

Lazarin L. Biokemijska karakterizacija proteina RRAS2 iz spužve Eunapius subterraneus ; Istraživačka nastava kemije [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; Zagreb: University of Zagreb, Faculty of Science; 2023 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:217:021274

L. Lazarin, "Biokemijska karakterizacija proteina RRAS2 iz spužve Eunapius subterraneus ; Istraživačka nastava kemije", Master's thesis, University of Zagreb, Faculty of Science, Zagreb; University of Zagreb, Faculty of Science, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:217:021274

Metadata
TitleBiokemijska karakterizacija proteina RRAS2 iz spužve Eunapius subterraneus ; Istraživačka nastava kemije
Title (english)Biochemical characterization of the RRAS2 protein from the sponge Eunapius subterraneus ; Inquiry-Based Chemistry Education
AuthorLeonarda Lazarin
MentorHelena Ćetković (mentor)
MentorDraginja Mrvoš-Sermek (mentor)
Committee memberDraginja Mrvoš-Sermek (predsjednik povjerenstva)
Committee memberJasmina Rokov Plavec (član povjerenstva)
Committee memberMirela Sertić Perić (član povjerenstva)
Committee memberRenata Matoničkin Kepčija (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
GranterUniversity of Zagreb
Faculty of Science
(Department of Chemistry)
Zagreb
Defense date and country2023-02-23, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
Rak je jedna od najrasprostranjenijih bolesti današnjice i često se javlja uslijed mutacija ili promjena u ekspresiji gena koji kodiraju za proteine uključene u kontrolu rasta i razvoja stanica. RRAS2 je primjer takvog proteina te pripada skupini malih GTPaza. Često je prekomjerno eksprimiran u različitim tipovima raka kod ljudi. Homolog proteina RRAS2 pronađen je i kod evolucijski puno starijih i jednostavnijih životinja – spužvi. Spužve predstavljaju dobar modelni organizam jer imaju jednostavnu morfologiju, a kompleksan genom, te većina gena iz spužvi pokazuje značajnu očuvanost s homolozima kod kralješnjaka. Kako bismo dobili bolji uvid u biokemijske karakteristike homologa RRAS2 iz spužve Eunapius subterraneus, klonirana je spužvina cDNA za RRAS2 te unesena u odgovarajući ekspresijski vektor. Napravljena je prekomjerna ekspresija rekombinantnog proteina u bakteriji Escherichia coli. Analizirana je i potvrđena intrinzična GTPazna aktivnost spužvinog proteina RRAS2, što ukazuje na očuvanost biokemijskih svojstava ovog onkogena. U metodičkom dijelu ovog rada dan je prijedlog nastavnog sata temeljen na istraživačkoj nastavi, za odabranu temu (Enzimi). Pregledana je stručna i udžbenička literatura, oblikovani su prikladni didaktičko-metodički materijali te su navedeni mogući problemi koji se mogu javiti tijekom izvedbe nastavnog sata istraživačkog učenja.
Abstract (english)
Cancer is one of the most widespread diseases nowadays and often occurs due to mutations or changes in the expression levels of genes that control cell growth and development. RRAS2 is one of such proteins and a small GTPase. The homologue of the human RRAS2 was found in evolutionarily much older and simpler animals – sponges. Despite their simple morphology, sponges have a complex genome with many genes very similar to their vertebrate homologues. To get a better insight into the biochemical characteristics of the RRAS2 homologue from the sponge Eunapius subterraneus, the cDNA for RRAS2 was cloned and inserted into an appropriate expression vector. E. coli cells were chemically transformed for the expression of the RRAS2 recombinant protein. The GTPase activity of the sponge RRAS2 was analyzed and confirmed, which indicates that the biochemical properties of this oncogene are evolutionary conserved. In the methodological part, a proposal of inquiry-based lesson, for the selected topic (Enzymes) is given. Professional and textbook literature was reviewed, appropriate didactic-methodical materials were designed, and possible problems that may arise during the implementation of the inquiry-based learning were highlighted.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:021274
ProjectNumber: IP-2019-04-5382
Title: Geni spužvi povezani s nastankom raka
Title: Cancer-related genes in sponges
Acronym: CanSponGE
Leader: Helena Ćetković
Jurisdiction: Croatia
Funder: HRZZ
Funding stream: IP
Study programmeTitle: Integrated undergraduate and graduate university study of Biology and Chemistry Education; specializations in: Integrated undergraduate and graduate university study of Biology and Chemistry Education
Course: Integrated undergraduate and graduate university study of Biology and Chemistry Education
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
Type of resourceText
File originBorn digital
Access conditionsEmbargoed access
Embargo expiration date: 2025-02-23
Terms of useLicence In copyright icon
Created on2023-06-14 13:21:11