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doctoral thesis
The role of microtubule-associated proteins in the generation and regulation of spindle shape
Zagreb: University of Zagreb, Faculty of Science, 2023. urn:nbn:hr:217:734850
Trupinić, Monika
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Trupinić, M. (2023). The role of microtubule-associated proteins in the generation and regulation of spindle shape (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:734850

Trupinić, Monika. "The role of microtubule-associated proteins in the generation and regulation of spindle shape." Doctoral thesis, University of Zagreb, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:217:734850

Trupinić, Monika. "The role of microtubule-associated proteins in the generation and regulation of spindle shape." Doctoral thesis, University of Zagreb, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:217:734850

Trupinić, M. (2023). 'The role of microtubule-associated proteins in the generation and regulation of spindle shape', Doctoral thesis, University of Zagreb, Faculty of Science, accessed 03 May 2024, https://urn.nsk.hr/urn:nbn:hr:217:734850

Trupinić M. The role of microtubule-associated proteins in the generation and regulation of spindle shape [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Science; 2023 [cited 2024 May 03] Available at: https://urn.nsk.hr/urn:nbn:hr:217:734850

M. Trupinić, "The role of microtubule-associated proteins in the generation and regulation of spindle shape", Doctoral thesis, University of Zagreb, Faculty of Science, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:217:734850

Metadata
TitleThe role of microtubule-associated proteins in the generation and regulation of spindle shape
Title (croatian)Uloga proteina vezanih na mikrotubule u stvaranju i regulaciji oblika diobenoga vretena
AuthorMonika Trupinić
MentorIva Marija Tolić (mentor)
Committee memberDragomira Majhen (predsjednik povjerenstva)
Committee memberMaja Matulić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2023-06-13, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Universal decimal classification
(UDC)		576 - Cellular and subcellular biology. Cytology
Abstract
In the mitotic spindle, a complex micro-structure responsible for the segregation of chromosomes, individual microtubule bundles obtain different shapes depending on the mechanical forces acting on them. In addition to linear forces, rotational forces are present in the spindle, reflected in the left-handed twisted shapes of microtubule bundles that make the spindle chiral. However, it is not known how twist changes throughout mitosis in non-tumor cells, or what are the molecular origins of the twist. This doctoral thesis shows that twist in non-tumor cells is most pronounced at the start of the anaphase, and can be affected by manipulating microtubule-associated proteins, such as motor proteins, but also proteins that crosslink or nucleate microtubule bundles. It also explores how spindles react to the external forces by widening or increasing the twist. To conclude, this thesis uncovers how bending and twisting moments are generated by similar molecular mechanisms and it proposes a physiological role for spindle chirality in allowing the spindle to absorb mechanical load.
Abstract (croatian)
Unutar diobenog vretena, složene mikro-strukture odgovorne za podijelu kromosoma, pojedinačni svežnjevi mikrotubula poprimaju različite oblike pod djelovanjem mehaničkih sila. U vretenu, osim linearnih sila, prisutne su i rotacijske sile koje se očituju kroz lijevo usmjereni zaokret svežnjeva mikrotubula što čini diobeno vreteno kiralnim. Međutim, nije poznato kako se takav zaokret mijenja kroz različite faze mitoze u netumorskim stanicama, niti koje su njegove molekularne osnove. U ovom doktorskom radu prikazano je kako je zaokret vretena najizraženiji na početku anafaze te podložan promjenama nakon manipulacije proteina vezanih na mikrotubule, kao što su motorni proteini, ali i proteini koji unakrsno vežu mikrotubule ili stvaraju nove. Također, prikazano je kako vretena reagiraju na pritisak vanjskih sila širenjem ili povećanjem zaokreta. Kao zaključak, ova teza prikazuje kako su slični molekularni mehanizmki odgovorni za stvaranje savijanja i zakretanja vretena te predlaže fiziolšku ulogu kiralnosti u očuvanju vretena nakon djelovanja mehaničkih sila.
Keywords
Keywords (croatian)
Languageenglish
URN:NBNurn:nbn:hr:217:734850
Promotion2023
Study programmeTitle: Biology
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2023-07-12 11:10:03