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master's thesis
Bioakumulacija i utjecaj srebrnih nanočestica na unutarstaničnu raspodjelu metala i metalotioneine u gujavice Eisenia fetida (Savigny, 1826)
Zagreb: University of Zagreb, Faculty of Science, 2016. urn:nbn:hr:217:091115
Horvat, Filip
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Horvat, F. (2016). Bioakumulacija i utjecaj srebrnih nanočestica na unutarstaničnu raspodjelu metala i metalotioneine u gujavice Eisenia fetida (Savigny, 1826) (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:091115

Horvat, Filip. "Bioakumulacija i utjecaj srebrnih nanočestica na unutarstaničnu raspodjelu metala i metalotioneine u gujavice Eisenia fetida (Savigny, 1826)." Master's thesis, University of Zagreb, Faculty of Science, 2016. https://urn.nsk.hr/urn:nbn:hr:217:091115

Horvat, Filip. "Bioakumulacija i utjecaj srebrnih nanočestica na unutarstaničnu raspodjelu metala i metalotioneine u gujavice Eisenia fetida (Savigny, 1826)." Master's thesis, University of Zagreb, Faculty of Science, 2016. https://urn.nsk.hr/urn:nbn:hr:217:091115

Horvat, F. (2016). 'Bioakumulacija i utjecaj srebrnih nanočestica na unutarstaničnu raspodjelu metala i metalotioneine u gujavice Eisenia fetida (Savigny, 1826)', Master's thesis, University of Zagreb, Faculty of Science, accessed 26 December 2024, https://urn.nsk.hr/urn:nbn:hr:217:091115

Horvat F. Bioakumulacija i utjecaj srebrnih nanočestica na unutarstaničnu raspodjelu metala i metalotioneine u gujavice Eisenia fetida (Savigny, 1826) [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2016 [cited 2024 December 26] Available at: https://urn.nsk.hr/urn:nbn:hr:217:091115

F. Horvat, "Bioakumulacija i utjecaj srebrnih nanočestica na unutarstaničnu raspodjelu metala i metalotioneine u gujavice Eisenia fetida (Savigny, 1826)", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:217:091115

Metadata
TitleBioakumulacija i utjecaj srebrnih nanočestica na unutarstaničnu raspodjelu metala i metalotioneine u gujavice Eisenia fetida (Savigny, 1826)
Title (english)Bioaccumulation and effect of silver nanoparticles on subcellular distribution of metals and on metallothoneins in earthworm Eisenia fetida (Savigny,1826)
AuthorFilip Horvat
MentorDušica Ivanković (mentor)
MentorAnamaria Štambuk (mentor)
Committee memberAnamaria Štambuk (predsjednik povjerenstva)
Committee memberŽeljka Vidaković-Cifrek (član povjerenstva)
Committee memberInga Marijanović (član povjerenstva)
Committee memberDunja Leljak-Levanić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2016-02-29, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Gujavica Eisenia fetida izlagana je povišenoj koncentraciji nanočestica odnosno ionskog srebra (AgNO3). Uspoređen je stupanj bioakumulacije te su praćene sličnosti i razlike u subcelularnoj i citosolnoj distribuciji srebra nakon izlaganja. Također je istraživan potencijal oba oblika srebra za pobudnu sintezu metalotioneina te utjecaj na raspodjelu nekoliko esencijalnih elemenata (Cu, Zn, Mn, Fe, Co, Mo i Se) i Cd između citosola i netopljive frakcije tkiva gujavica. Također je napravljena analiza distribucije srebra i ostalih elemenata između citosolnih liganda različite molekulske mase razdvojenih pomoću SE-HPLC. Pokazano je da kod oba oblika srebra dolazi do bioakumulacije u tkivima gujavica. Razina bioakumulacije nekoliko je puta veća kod ionskog oblika. Postoje vidljive razlike u unutarstaničnoj i citosolnoj raspodjeli srebra između dva oblika. Nanočestice ne induciraju sintezu metalotioneina, a ionsko srebro ju inducira tek pri najvećoj koncetraciji izlaganja, no dio unutarstaničnog srebra vezan je na postojeće metalotioneine u stanici. Kvantitativne i kvalitativne promjene u unutarstaničnoj distribuciji esencijalnih elemenata željeza, kobalta i molibdena pokazuju potencijal kao biomarkeri izloženosti nanočesticama odnosno ionskom srebru.
Abstract (english)
Earthworm Eisenia fetida was exposed to increased concentrations of silver in nanoparticle and ionic (AgNO3) form. The comparison of bioaccumulation between two forms of silver, along with comparison of similarities and differences in subcellular and cytosolic distribution of silver after exposure were made. The potential of both silver forms to induce the synthesis of metallothioneins and change the distribution of chosen essential elements (Cu, Zn, Mn, Fe, Co, Mo, Se) and Cd between the particulate (pellet) and cytosolic fraction of E. fetida tissue was investigated. The distribution of chosen elements among different molecular mass cytosolic fractions separated by size exclusion HPLC was also analysed. It was shown that both silver forms accumulate in earthworm tissues. Bioaccumulation levels were higher in earthworms exposed to ionic silver when compared to nanoparticle silver. There are significant differences in cellular and cytosolic distribution of silver between these two forms of exposure. Silver nanoparticles are not inducing metallothionein synthesis while ionic silver induces it, but only in the highest concentration of exposure. Part of intracellular silver is bound to metallothioneins which are already expressed in cells. The quantitative and qualitative differences in intracellular distribution of essential elements iron, cobalt and molybdenum show potential as biomarkers of nanoparticle and ionic silver form exposure.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:091115
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-05-05 11:46:25