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master's thesis
Optimizacija uvjeta za trodimenzionalni rast hrskavice in vitro
Zagreb: University of Zagreb, Faculty of Science, 2016. urn:nbn:hr:217:793257
Jagrić, Mihaela
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Jagrić, M. (2016). Optimizacija uvjeta za trodimenzionalni rast hrskavice in vitro (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:793257

Jagrić, Mihaela. "Optimizacija uvjeta za trodimenzionalni rast hrskavice in vitro." Master's thesis, University of Zagreb, Faculty of Science, 2016. https://urn.nsk.hr/urn:nbn:hr:217:793257

Jagrić, Mihaela. "Optimizacija uvjeta za trodimenzionalni rast hrskavice in vitro." Master's thesis, University of Zagreb, Faculty of Science, 2016. https://urn.nsk.hr/urn:nbn:hr:217:793257

Jagrić, M. (2016). 'Optimizacija uvjeta za trodimenzionalni rast hrskavice in vitro', Master's thesis, University of Zagreb, Faculty of Science, accessed 22 December 2024, https://urn.nsk.hr/urn:nbn:hr:217:793257

Jagrić M. Optimizacija uvjeta za trodimenzionalni rast hrskavice in vitro [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2016 [cited 2024 December 22] Available at: https://urn.nsk.hr/urn:nbn:hr:217:793257

M. Jagrić, "Optimizacija uvjeta za trodimenzionalni rast hrskavice in vitro", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:217:793257

Metadata
TitleOptimizacija uvjeta za trodimenzionalni rast hrskavice in vitro
Title (english)Optimization of conditions for in vitro three-dimensional cartilage growth
AuthorMihaela Jagrić
MentorInga Marijanović (mentor)
Committee memberInga Marijanović (predsjednik povjerenstva)
Committee memberJasna Hrenović (član povjerenstva)
Committee memberDuje Lisičić (član povjerenstva)
Committee memberMaja Matulić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2016-02-29, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Artikularna hrskavica je tkivo koje nema sposobnost spontane regeneracije. Tkivno inženjerstvo, kao novo područje regenerativne medicine, nudi potencijalna učinkovita rješenja u liječenju ozljeda i oštećenja artikularne hrskavice. Cilj ovoga istraživanja bio je optimizirati uvjete za trodimenzionalni uzgoj hrskavice in vitro. U istraživanju su korištena tri različita tipa staničnih kultura: hondrociti, mezenhimske matične stanice iz koštane srži (hBMSCs) te kombinacija hondrocita i hBMSCs u omjeru 2:1. Stanice su uzgajane u diferencijacijskom mediju, inkorporirane u RADA peptidni hidrogel, u normoksičnim (20% O2) ili hipoksičnim (5% O2) uvjetima. Dexametazon, jedna od komponenti diferencijacijskoga medija, bila je dodana u medij ili inkorporirana sa stanicama u peptidnom hidrogelu. Nakon 19 dana metodom kvantitativnog PCR-a ispitana je razina ekspresije gena SOX9 i ACAN, karakterističnih za hrskavicu. Među korištenim tipovima stanica hondrociti su pokazali najviši, a hBMSCs najniži hondrogeni potencijal. Hipoksija je značajno poboljšala hondrogenezu jedino u slučaju hondrocita, dok izvor dexametazona nije bitno utjecao na razinu ekspresije gena SOX9 i ACAN.
Abstract (english)
Articular cartilage is a tissue that shows no capacity for effective spontaneous regeneration. Tissue engineering, as a new important field of regenerative medicine, emerges as potential effective solution for treatment of articular cartilage injuries and defects. The aim of this study was to optimize conditions for 3D in vitro chondrogenesis. In the experiment, three different cell culture types were used: chondrocytes, human bone marrow mesenchymal stem cells (hBMSCs) and combination of chondrocytes and hBMSCs in 2:1 ratio. Cells were grown in a chondrogenic medium, incorporated in a peptide hydrogel RADA, in either normoxic (20% O2) or hypoxic (5% O2) conditions. Dexamethasone, one of the chondrogenic medium components, was added in a differentiation medium or it was incorporated with cells within a peptide hydrogel. After 19 days, expression levels of two cartilage marker genes, SOX9 and ACAN, were evaluated by quantitative PCR. Chondrocytes showed the highest and hBMSCs the lowest chondrogenic potential among used cell types. Hypoxia enhanced chondrogenesis significantly only in the case of chondrocytes, while dexamethasone source did not considerably influence on expression levels of SOX and ACAN genes.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:793257
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-05-05 11:49:44