prikaz prve stranice dokumenta Uloga reaktivnih kisikovih oblika u odgovoru krumpira (Solanum tuberosum) na infekciju viroidom vretenastog gomolja krumpira (PSTVd)
Embargo period 2025-07-14
master's thesis
Uloga reaktivnih kisikovih oblika u odgovoru krumpira (Solanum tuberosum) na infekciju viroidom vretenastog gomolja krumpira (PSTVd)
Zagreb: University of Zagreb, Faculty of Science, 2023. urn:nbn:hr:217:452434
Kumek, Lucija
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Kumek, L. (2023). Uloga reaktivnih kisikovih oblika u odgovoru krumpira (Solanum tuberosum) na infekciju viroidom vretenastog gomolja krumpira (PSTVd) (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:452434

Kumek, Lucija. "Uloga reaktivnih kisikovih oblika u odgovoru krumpira (Solanum tuberosum) na infekciju viroidom vretenastog gomolja krumpira (PSTVd)." Master's thesis, University of Zagreb, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:217:452434

Kumek, Lucija. "Uloga reaktivnih kisikovih oblika u odgovoru krumpira (Solanum tuberosum) na infekciju viroidom vretenastog gomolja krumpira (PSTVd)." Master's thesis, University of Zagreb, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:217:452434

Kumek, L. (2023). 'Uloga reaktivnih kisikovih oblika u odgovoru krumpira (Solanum tuberosum) na infekciju viroidom vretenastog gomolja krumpira (PSTVd)', Master's thesis, University of Zagreb, Faculty of Science, accessed 21 May 2024, https://urn.nsk.hr/urn:nbn:hr:217:452434

Kumek L. Uloga reaktivnih kisikovih oblika u odgovoru krumpira (Solanum tuberosum) na infekciju viroidom vretenastog gomolja krumpira (PSTVd) [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2023 [cited 2024 May 21] Available at: https://urn.nsk.hr/urn:nbn:hr:217:452434

L. Kumek, "Uloga reaktivnih kisikovih oblika u odgovoru krumpira (Solanum tuberosum) na infekciju viroidom vretenastog gomolja krumpira (PSTVd)", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:217:452434

Metadata
TitleUloga reaktivnih kisikovih oblika u odgovoru krumpira (Solanum tuberosum) na infekciju viroidom vretenastog gomolja krumpira (PSTVd)
Title (english)The role of reactive oxygen species in response of potato (Solanum tuberosum) to infection with potato spindle tuber viroid (PSTVd)
AuthorLucija Kumek
MentorSnježana Mihaljević (mentor)
MentorDunja Leljak-Levanić (komentor)
Committee memberDunja Leljak-Levanić (predsjednik povjerenstva)
Committee memberŽeljka Vidaković-Cifrek (član povjerenstva)
Committee memberNataša Bauer (član povjerenstva)
Committee memberNenad Malenica (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2023-07-14, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Reaktivni kisikovi oblici (ROS) i salicilna kiselina (SA) igraju važnu ulogu u obrambenom odgovoru biljaka na patogene. U ovom radu istražena je uloga ROS-ova i SA u interakciji biljaka krumpira (Solanum tuberosum) i viroida vretenastog gomolja krumpira (PSTVd). Kvantificiran je sadržaj vodikovog peroksida (H2O2) u listovima biljaka inokuliranih viroidom PSTVd i kontrolnih biljaka linije divljeg tipa i transgenične linije NahG, te je određen učinak infekcije na akumulaciju H2O2 u različitim vremenskim točkama nakon inokulacije. Istovremeno je analizirana ekspresija gena uključenih u signalne puteve ROS-ova, uključujući gene za odabrane katalaze, peroksidaze i proteinske kinaze aktivirane mitogenima (MAPK). Nadalje, uloga SA u interakciji krumpir-PSTVd istražena je praćenjem učinka egzogenog tretmana s njenim sintetskim analogom, 2,6- dikloroizonikotinskom kiselinom (INA), na akumulaciju H2O2 i promjene u ekspresiji gena povezanih s antioksidacijskim odgovorom reguliranim SA-om. Rezultati ukazuju da odgovor krumpira na infekciju viroidom PSTVd uključuje akumulaciju H2O2 i pokretanje antioksidacijskog odgovora reguliranog SA-om koji dovodi do transkripcijske aktivacije peroksidaza i kinaza MAPK. Opažen je pozitivan učinak tretmana s INA-om na imuni odgovor krumpira, posebice u liniji NahG. Saznanja dobivena ovim istraživanjem pridonose boljem razumijevanju molekularnih mehanizama uključenih u interakciju biljaka i viroida.
Abstract (english)
Reactive oxygen species (ROS) and salicylic acid (SA) play a key role in plants' defense response against pathogens. Herein, the role of ROS and SA in the interaction between potato (Solanum tuberosum) plants and potato spindle tuber viroid (PSTVd) was investigated. The hydrogen peroxide content (H2O2) in leaves of control and PSTVd-inoculated wild-type and transgenic NahG plants was quantified at different time points after inoculation, and the effect of infection on H2O2 accumulation was determined. Simultaneously, the expression of genes involved in ROS signaling pathways, including catalase, peroxidases and mitogen-activated protein kinases (MAPK), was analyzed. Furthermore, the role of SA in the potato-PSTVd interaction was investigated by monitoring the effect of exogenous treatment with 2,6-dichloroisonicotinic acid (INA), a synthetic analog of SA, on H2O2 accumulation and changes in the expression of genes associated with SAregulated antioxidant response. The results indicate that the response of potato plants to PSTVd infection involves accumulation of H2O2 and the activation of SA-regulated antioxidant responses, leading to transcriptional activation of peroxidases and MAPK. A positive effect of INA treatment on potato immunity, particularly in NahG lines was observed. The findings from this study contribute to a better understanding of the molecular mechanisms underlying plant-pathogen interactions.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:452434
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsEmbargoed access
Embargo expiration date: 2025-07-14
Terms of useLicence In copyright icon
Created on2023-07-26 08:14:47