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undergraduate thesis
Utjecaj proteina DnaK na naivnu CRISPRCas adaptaciju u bakteriji Escherichia coli
Zagreb: University of Zagreb, Faculty of Science, 2023. urn:nbn:hr:217:117144
Jurada, Mario
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Jurada, M. (2023). Utjecaj proteina DnaK na naivnu CRISPRCas adaptaciju u bakteriji Escherichia coli (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:117144

Jurada, Mario. "Utjecaj proteina DnaK na naivnu CRISPRCas adaptaciju u bakteriji Escherichia coli." Undergraduate thesis, University of Zagreb, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:217:117144

Jurada, Mario. "Utjecaj proteina DnaK na naivnu CRISPRCas adaptaciju u bakteriji Escherichia coli." Undergraduate thesis, University of Zagreb, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:217:117144

Jurada, M. (2023). 'Utjecaj proteina DnaK na naivnu CRISPRCas adaptaciju u bakteriji Escherichia coli', Undergraduate thesis, University of Zagreb, Faculty of Science, accessed 21 May 2024, https://urn.nsk.hr/urn:nbn:hr:217:117144

Jurada M. Utjecaj proteina DnaK na naivnu CRISPRCas adaptaciju u bakteriji Escherichia coli [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Science; 2023 [cited 2024 May 21] Available at: https://urn.nsk.hr/urn:nbn:hr:217:117144

M. Jurada, "Utjecaj proteina DnaK na naivnu CRISPRCas adaptaciju u bakteriji Escherichia coli", Undergraduate thesis, University of Zagreb, Faculty of Science, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:217:117144

Metadata
TitleUtjecaj proteina DnaK na naivnu CRISPRCas adaptaciju u bakteriji Escherichia coli
Title (english)The effect of DnaK protein on naïve CRISPR-Cas adaptation in Escherichia coli
AuthorMario Jurada
MentorIvana Ivančić Baće (mentor)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2023-09-07, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Adaptivni imunosni sustav, CRISPR-Cas, moćno je oružje u obrani protiv invazivnih genetičkih elementa, poput bakteriofaga i plazmida. Efektivnost tog sustava uvelike ovisi o njegovoj mogućnosti raspoznavanja vlastite i strane DNA. Nakon prepoznavanja strane DNA, u procesu naivne adaptacije, kompleks Cas1–Cas2 iz strane DNA stvara razmaknice koje ugrađuje u Lokus CRISPR. Dugo vremena nije bilo poznato na koji način Cas1–Cas2 raspoznaje stranu od vlastite DNA. U nedavnim istraživanjima otkriveno je da protein DnaK, koji inače ima ulogu šaperona i jedan je od najvažnijih proteina toplinskog šoka, igra važnu ulogu u prostornoj i vremenskoj regulaciji naivne adaptacije. U ovom radu ponovljeni su neki od eksperimenata iz nedavnih istraživanja koji su dokazali utjecaj DnaK na aktivnost naivne adaptacije. Praćenjem efikasnosti naivne adaptacije u lizogenim sojevima E. coli pokazao sam da DnaK ima negativan utjecaj na naivnu adaptaciju, tj. da smanjuje stopu ugradnje novih razmaknica. Ti rezultati su u skladu s nedavnim istraživanjima koja sugeriraju da protein DnaK sprječava ugradnju vlastite DNA inhibicijom kompleksa Cas1–Cas2 prilikom naivne adaptacije.
Abstract (english)
The adaptive immune system, CRISPR-Cas, is a powerful weapon in defense against invasive genetic elements such as bacteriophages and plasmids. The effectiveness of this system greatly depends on its ability to distinguish between self and foreign DNA. Upon recognizing foreign DNA, in the process of naïve adaptation, the Cas1–Cas2 complex generates spacers from foreign DNA, which are then incorporated into the CRISPR locus. For a long time, it was not known how Cas1– Cas2 distinguishes between foreign and self-DNA. Recent research has revealed that the protein DnaK, which normally acts as a chaperone and is one of the key heat shock proteins, plays an important role in spatial and temporal regulation of naive adaptation. This study replicated some of the experiments from recent research, which demonstrated the impact of DnaK on the activity of naïve adaptation. By monitoring the efficiency of naive adaptation in lysogenic strains of E. coli, I show that DnaK has a negative influence on naïve adaptation, i.e., it reduces the rate of incorporating new spacers. These results are consistent with recent research, where it is suggested that DnaK prevents acqusition of host DNA by inhibiting the Cas1–Cas2 complex during naive adaptation.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:117144
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) molekularne biologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2023-09-12 10:57:21