prikaz prve stranice dokumenta Glikozilacija IgG-a u žena: Pubertet, trudnoća i menopauza
Embargo period 2025-09-14
undergraduate thesis
Glikozilacija IgG-a u žena: Pubertet, trudnoća i menopauza
Zagreb: University of Zagreb, Faculty of Science, 2023. urn:nbn:hr:217:185283
Perinović, Maja
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Perinović, M. (2023). Glikozilacija IgG-a u žena: Pubertet, trudnoća i menopauza (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:185283

Perinović, Maja. "Glikozilacija IgG-a u žena: Pubertet, trudnoća i menopauza." Undergraduate thesis, University of Zagreb, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:217:185283

Perinović, Maja. "Glikozilacija IgG-a u žena: Pubertet, trudnoća i menopauza." Undergraduate thesis, University of Zagreb, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:217:185283

Perinović, M. (2023). 'Glikozilacija IgG-a u žena: Pubertet, trudnoća i menopauza', Undergraduate thesis, University of Zagreb, Faculty of Science, accessed 22 May 2024, https://urn.nsk.hr/urn:nbn:hr:217:185283

Perinović M. Glikozilacija IgG-a u žena: Pubertet, trudnoća i menopauza [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Science; 2023 [cited 2024 May 22] Available at: https://urn.nsk.hr/urn:nbn:hr:217:185283

M. Perinović, "Glikozilacija IgG-a u žena: Pubertet, trudnoća i menopauza", Undergraduate thesis, University of Zagreb, Faculty of Science, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:217:185283

Metadata
TitleGlikozilacija IgG-a u žena: Pubertet, trudnoća i menopauza
Title (english)IgG glycosylation in women: Puberty, pregnancy, and menopause
AuthorMaja Perinović
MentorVlatka Zoldoš (mentor)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2023-09-14, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Imunoglobulin G (IgG) najzastupljenija je izoforma imunoglobulina u krvotoku čovjeka. Nglikani vezani na IgG utječu na efektorske funkcije ovog antitijela i na taj način reguliraju imunološki odgovor. Kompozicija IgG glikoma značajno se mijenja u pubertetu, trudnoći i menopauzi kao direktna posljedica promjena u koncentraciji estrogena. Međutim, točni mehanizmi kojima estrogen dovodi do promjene glikozilacije IgG-a nisu poznati. Cjelogenomske studije povezanosti su otkrile kako je gen ABCF2 reguliran estrogenom uključen u glikozilaciju IgG-a. Kako bismo potvrdili ulogu gena ABCF2 u tom procesu, povećali smo njegovu ekspresiju u staničnoj liniji FreeStyle-293-F koja stabilno eksprimira fuzijski protein dCas9-VPR i luči molekule IgG u stanični medij. Povećana ekspresija gena ABCF2 nakon manipulacije stanica alatom CRISPR/dCas9-VPR rezultirala je povećanom razinom galaktoziliranih glikoformi IgG-a (G1 i G2) i istovremenim smanjenjem agalaktoziliranih glikoformi IgG-a (G0) što sugerira da ABCF2, gen reguliran estrogenom, najvjerojanije sudjeluje u regulaciji glikozilacije IgG-a.
Abstract (english)
Immunoglobulin G (IgG) is the most abundant immunoglobulin isoform in the human bloodstream. Different N-glycans attached to IgG directly influence this antibody's effector functions and regulate the immune response. IgG glycome composition is changed significantly during puberty, pregnancy, and menopause, as a direct consequence of the change in estrogen concentration. However, the exact mechanisms by which estrogen modulates IgG glycosylation are unknown. Genome-wide association studies have shown that the estrogen-regulated gene ABCF2 is associated with IgG glycosylation. To functionally validate the role of the ABCF2 gene in this process, I overexpressed it in the FreeStyle-293-F cell line that stably expresses the dCas9- VPR fusion protein and secretes IgG. Overexpression of the ABCF2 gene resulted in an increased level of galactosylated IgG glycoforms (G1 and G2) and a simultaneous decrease in agalactosylated glycoforms of IgG (G0), suggesting that estrogen-regulated gene ABCF2 has a role in the regulation of IgG glycosylation.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:185283
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) molekularne biologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsEmbargoed access
Embargo expiration date: 2025-09-14
Terms of useLicence In copyright icon
Created on2023-09-15 08:54:35