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master's thesis
Ispitivanje interakcija peptidnih derivata fenantridina s DNA i RNA
Zagreb: University of Zagreb, Faculty of Science, 2023. urn:nbn:hr:217:483045
Jurić, Ivona
University of Zagreb
Faculty of Science
Department of Chemistry

Institutional repository: Repository of the Faculty of Science

Cite this document

Jurić, I. (2023). Ispitivanje interakcija peptidnih derivata fenantridina s DNA i RNA (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:483045

Jurić, Ivona. "Ispitivanje interakcija peptidnih derivata fenantridina s DNA i RNA." Master's thesis, University of Zagreb, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:217:483045

Jurić, Ivona. "Ispitivanje interakcija peptidnih derivata fenantridina s DNA i RNA." Master's thesis, University of Zagreb, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:217:483045

Jurić, I. (2023). 'Ispitivanje interakcija peptidnih derivata fenantridina s DNA i RNA', Master's thesis, University of Zagreb, Faculty of Science, accessed 20 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:483045

Jurić I. Ispitivanje interakcija peptidnih derivata fenantridina s DNA i RNA [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2023 [cited 2024 November 20] Available at: https://urn.nsk.hr/urn:nbn:hr:217:483045

I. Jurić, "Ispitivanje interakcija peptidnih derivata fenantridina s DNA i RNA", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:217:483045

Metadata
TitleIspitivanje interakcija peptidnih derivata fenantridina s DNA i RNA
Title (english)Study of interactions of peptide phenanthridine derivatives with DNA and RNA
AuthorIvona Jurić
MentorMarijana Radić Stojković (mentor)
Committee memberIva Juranović Cindrić (predsjednik povjerenstva)
Committee memberIvica Đilović (član povjerenstva)
Committee memberĐani Škalamera (član povjerenstva)
Committee memberAdriana Kenđel (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Chemistry)
Zagreb
Defense date and country2023-09-28, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
U okviru ovog diplomskog rada spektroskopski su okarakterizirane vodene otopine tri nova derivata fenantridina s aminokiselinskim supstituentima (glicin, lizin) i gvanidinokarbonilpirolnom jedinicom. Fenantridin je poznati fluorescentni marker za polinukleotide i proteine dok aminokiselinski supstituenti pružaju dodatne mogućnosti za interakcije s biološkim makromolekulama. Konstante stabilnosti kompleksa ispitivanih spojeva s različitim DNA i RNA polinukleotidima određene su metodom fluorescencijske spektroskopije. Način vezanja peptidnih derivata fenantridina na DNA i RNA polinukleotide određen je eksperimentima temperature mekšanja polinukleotida i spektroskopijom cirkularnog dikroizma. Konstante stabilnosti kompleksa ispitivanih derivata s polinukleotidima izračunate su pomoću Scatchardove jednadžbe metodom nelinearne regresije. Dobiveni rezultati ukazuju na vezanje spojeva (najvjerojatnije u obliku dimera) u utore DNA i RNA te uzduž fosfatne okosnice polinukleotida.
Abstract (english)
In the scope of this thesis, aqueous solutions of three new phenanthridine derivatives with amino acid substituents (glycine, lysine) and a guanidinocarbonylpyrrole unit were spectroscopically characterized. Phenanthridine is a known fluorescent marker for polynucleotides and proteins. Amino acid substituents provide additional possibilities for interactions with biological macromolecules. The stability constants of the complexes of the studied compounds with different DNA and RNA polynucleotides were determined by fluorescence spectroscopy. The binding mode of peptide phenanthridine derivatives to DNA and RNA polynucleotides was determined by thermal melting experiments and circular dichroism spectroscopy. The stability constants of the compound-polynucleotide complexes were calculated by the Scatchard equation using the nonlinear regression method. The obtained results indicate the binding of compounds (most likely in the form of dimers) in the grooves of DNA and RNA and along the phosphate backbone of polynucleotides.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:483045
ProjectNumber: IP-2018-01-4694
Title: Molekularno prepoznavanje DNA:RNA hibridnih i višelančanih struktura u bioanalitičkim i in vitro sustavima
Acronym: DNARNAHyB-MolBio
Leader: Marijana Radić Stojković
Jurisdiction: Croatia
Funder: HRZZ
Funding stream: IP
Study programmeTitle: Graduate university studies in chemistry; specializations in: Research programme
Course: Research programme
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2023-10-13 12:22:11