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master's thesis
Heterogenost gena BZLF1 i BRLF1 virusa Epstein-Barr u infektivnoj mononukleozi
Zagreb: University of Zagreb, Faculty of Science, 2023. urn:nbn:hr:217:940014
Božić, Filip
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Božić, F. (2023). Heterogenost gena BZLF1 i BRLF1 virusa Epstein-Barr u infektivnoj mononukleozi (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:940014

Božić, Filip. "Heterogenost gena BZLF1 i BRLF1 virusa Epstein-Barr u infektivnoj mononukleozi." Master's thesis, University of Zagreb, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:217:940014

Božić, Filip. "Heterogenost gena BZLF1 i BRLF1 virusa Epstein-Barr u infektivnoj mononukleozi." Master's thesis, University of Zagreb, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:217:940014

Božić, F. (2023). 'Heterogenost gena BZLF1 i BRLF1 virusa Epstein-Barr u infektivnoj mononukleozi', Master's thesis, University of Zagreb, Faculty of Science, accessed 23 December 2024, https://urn.nsk.hr/urn:nbn:hr:217:940014

Božić F. Heterogenost gena BZLF1 i BRLF1 virusa Epstein-Barr u infektivnoj mononukleozi [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2023 [cited 2024 December 23] Available at: https://urn.nsk.hr/urn:nbn:hr:217:940014

F. Božić, "Heterogenost gena BZLF1 i BRLF1 virusa Epstein-Barr u infektivnoj mononukleozi", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:217:940014

Metadata
TitleHeterogenost gena BZLF1 i BRLF1 virusa Epstein-Barr u infektivnoj mononukleozi
Title (english)Heterogeneity of Epstein-Barr virus BZLF1 and BRLF1 genes in infectious mononucleosis
AuthorFilip Božić
MentorSnježana Židovec Lepej (mentor)
Committee memberSnježana Židovec Lepej (predsjednik povjerenstva)
Committee memberPetra Korać (član povjerenstva)
Committee memberDijana Škorić (član povjerenstva)
Committee memberTomislav Ivanković (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2023-09-26, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Virus Epstein-Barr (EBV) ili ljudski gamaherpesvirus 4 je sveprisutni DNA-virus koji zaražava više od 90% odraslih osoba diljem svijeta. Akutna infekcija ovim virusom može uzrokovati infektivnu mononukleozu, a latentna infekcija povezana je s razvojem malignih bolesti čovjeka. Replikacijski ciklus virusa EBV uključuje litičku i latentnu fazu u B-limfocitima, pri čemu je regulacija reaktivacije virusa iz latentne faze regulirana ekspresijom vrlo ranih litičkih gena BZLF1 i BRLF1. Preliminarna istraživanja polimorfizama ovih gena u izolatima virusa EBV iz nekoliko geografskih regija ukazuju na postojanje geografski ograničenih varijanti i varijanti povezanih s razvojem pojedinih malignih bolesti. Cilj ovog istraživanja bio je identificirati polimorfizme gena BRLF1 i BZLF1 u akutnoj infekciji EBVom u Hrvatskoj kako bi se proširilo znanje o genskoj varijabilnosti EBV-a na ovom području. U istraživanju su sekvencirani geni BZLF1 i BRLF1 virusa EBV, a istraživanje je provedeno na modelu infektivne mononukleoze. Analizom sekvenci detektirane su dvije varijante gena BZLF1, BZLF1-A2 i BZLF1-C koje su prethodno opisane u literaturi, ali i dva poptuno nova uzorka mutacija gena BZLF1, odnosno četiri uzorka mutacija gena BRLF1 koji prethodno nisu bili opisani u literaturi. Rezultati ovog istraživanja pokazali su postojanje novih genskih varijanti EBV-a te ukazuju na potrebu dodatnih istraživanja istih.
Abstract (english)
Epstein-Barr virus (EBV) or human gammaherpesvirus 4 is a ubiquitous DNA virus that infects more than 90% of adults worldwide. Acute EBV infection can cause infectious mononucleosis while latent infection can also lead to the development of malignant diseases in humans. EBV replication cycle in B-lymphocytes includes lytic and latent stages with periodic reactivation being regulated by the expression of the very early lytic genes BZLF1 and BRLF1. Preliminary data on polymorphisms of these two genes suggest the existence of geographically-limited variants as well as possible associaton with particular malignant diseases associated with EBV infection. The aim of this study was to identify BRLF1 and BZLF1 gene polymorphisms in acute EBV infection in Croatia in order to expand the knowledge on the genetic variability of EBV in this region. The study was performed by using infecious mononucleosis as a model and it involved sequencing of the BZLF1 gene and the subterminal and terminal regions of the BRLF1 gene. Sequence analysis detected two previously defined variants of the BZLF1 gene (BZLF1-A2 and BZLF1-C), as well as two and four mutation patterns of the BZLF1 and BRLF1 genes respectively, that have not been described previously. The results of this study have shown the existence of new EBV gene variants leading to new research opportunities in this field.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:940014
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2024-09-26
Terms of useLicence In copyright icon
Created on2023-10-16 10:08:24