prikaz prve stranice dokumenta Učinak probiotičkog soja Lactobacillus sp. i njegovih egzopolisaharida na upalni proces u crijevu miša soja C57BL/6J
Embargo period 2025-09-25
master's thesis
Učinak probiotičkog soja Lactobacillus sp. i njegovih egzopolisaharida na upalni proces u crijevu miša soja C57BL/6J
Zagreb: University of Zagreb, Faculty of Science, 2023. urn:nbn:hr:217:907408
Toljanić, Barbara
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Toljanić, B. (2023). Učinak probiotičkog soja Lactobacillus sp. i njegovih egzopolisaharida na upalni proces u crijevu miša soja C57BL/6J (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:907408

Toljanić, Barbara. "Učinak probiotičkog soja Lactobacillus sp. i njegovih egzopolisaharida na upalni proces u crijevu miša soja C57BL/6J." Master's thesis, University of Zagreb, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:217:907408

Toljanić, Barbara. "Učinak probiotičkog soja Lactobacillus sp. i njegovih egzopolisaharida na upalni proces u crijevu miša soja C57BL/6J." Master's thesis, University of Zagreb, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:217:907408

Toljanić, B. (2023). 'Učinak probiotičkog soja Lactobacillus sp. i njegovih egzopolisaharida na upalni proces u crijevu miša soja C57BL/6J', Master's thesis, University of Zagreb, Faculty of Science, accessed 23 December 2024, https://urn.nsk.hr/urn:nbn:hr:217:907408

Toljanić B. Učinak probiotičkog soja Lactobacillus sp. i njegovih egzopolisaharida na upalni proces u crijevu miša soja C57BL/6J [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2023 [cited 2024 December 23] Available at: https://urn.nsk.hr/urn:nbn:hr:217:907408

B. Toljanić, "Učinak probiotičkog soja Lactobacillus sp. i njegovih egzopolisaharida na upalni proces u crijevu miša soja C57BL/6J", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:217:907408

Metadata
TitleUčinak probiotičkog soja Lactobacillus sp. i njegovih egzopolisaharida na upalni proces u crijevu miša soja C57BL/6J
Title (english)The effect of the probiotic strain Lactobacillus sp. and its exopolysaccharides on the inflammatory process in the intestine of C57BL/6J mouse
AuthorBarbara Toljanić
MentorMirela Baus Lončar (mentor)
MentorNada Oršolić (komentor)
Committee memberNada Oršolić (predsjednik povjerenstva)
Committee memberRomana Gračan (član povjerenstva)
Committee memberMaja Matulić (član povjerenstva)
Committee memberDomagoj Đikić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2023-09-25, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Ulcerozni kolitis je kronična upala debelog crijeva koja zahvaća sluznicu. Ulcerozni kolitis obilježen je smanjenjem tjelesne težine, proljevom, krvavom stolicom, patohistološkim promjenama na sluznici crijeva te različitim patofiziološkim procesima kao što je kronična upala, oksidativni stres, stres endoplazmatskog retikuluma. Budući da je jedan od glavnih čimbenika ove bolesti disbioza, odnosno smanjena raznolikost bakterija u crijevima, danas se kao mogući pristup liječenja ulceroznog kolitisa istražuje obrada probioticima. Cilj ovog istraživanja bio je utvrditi kakav utjecaj ima probiotički soj Limosilactobacillus fermentum i njegovi egzopolisaharidi na dekstran natrij sulfatom (DSS) inducirani kolitis kod miševa soja C57BL/6J. Indeks razvoja kolitisa praćen je mjerenjem gubitka težine, kozistencije stolice i krvarenja, a posljedice bolesti analizom hematoloških, histoloških i molekularnih odrednica metodom kvantitativne lančane reakcije polimerazom (qPCR). Rezultati ukazuju na djelomočno pozitivan učinak probiotičkog soja Limosilactobacillus fermentum i njegovi egzopolisaharida na DSS-inducirani kolitis miševa soja C57BL/6J kroz smanjenu razinu upale i krvarenja te regeneraciju tkiva sluznice
Abstract (english)
Ulcerative colitis is a chronic inflammation of the large intestine restricted to the mucosa. Ulcerative colitis is characterized by weight loss, diarrhea, bloody stools, pathohistological changes in the intestinal mucosa and various pathophysiological processes such as chronic inflammation, oxidative stress, and endoplasmic reticulum stress. Since one of the main factors of this disease is dysbiosis, which is a reduced diversity of bacteria in the gut, today the use of probiotics is being investigated as a potential approach for treating ulcerative colitis. The aim of this research was to determine the impact of the probiotic strain Limosilactobacillus fermentum and its exopolysaccharides on dextran sodium sulfate (DSS) induced colitis in C57BL/6J mouse. The colitis activity index was monitored through measurements of weight loss, stool consistency, and bleeding, while the disease outcomes were analyzed using hematological, histological, and molecular markers through quantitative polymerase chain reaction (qPCR). The results indicate a partially positive effect of the probiotic strain Limosilactobacillus fermentum and its exopolysaccharides on DSS-induced colitis in C57BL/6J mice, manifested by reduced inflammation and bleeding, as well as mucosal tissue regeneration.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:907408
Study programmeTitle: Experimental Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
Type of resourceText
File originBorn digital
Access conditionsEmbargoed access
Embargo expiration date: 2025-09-25
Terms of useLicence In copyright icon
Created on2023-10-16 10:19:53