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master's thesis
Polimorfizam gena SULT2A1 i koncentracija dehidroepiandrosteron sulfata (DHEAS) u plazmi osoba s neurokognitivnim poremećajem
Zagreb: University of Zagreb, Faculty of Science, 2023. urn:nbn:hr:217:651986
Balić, Nikola
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Balić, N. (2023). Polimorfizam gena SULT2A1 i koncentracija dehidroepiandrosteron sulfata (DHEAS) u plazmi osoba s neurokognitivnim poremećajem (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:651986

Balić, Nikola. "Polimorfizam gena SULT2A1 i koncentracija dehidroepiandrosteron sulfata (DHEAS) u plazmi osoba s neurokognitivnim poremećajem." Master's thesis, University of Zagreb, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:217:651986

Balić, Nikola. "Polimorfizam gena SULT2A1 i koncentracija dehidroepiandrosteron sulfata (DHEAS) u plazmi osoba s neurokognitivnim poremećajem." Master's thesis, University of Zagreb, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:217:651986

Balić, N. (2023). 'Polimorfizam gena SULT2A1 i koncentracija dehidroepiandrosteron sulfata (DHEAS) u plazmi osoba s neurokognitivnim poremećajem', Master's thesis, University of Zagreb, Faculty of Science, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:651986

Balić N. Polimorfizam gena SULT2A1 i koncentracija dehidroepiandrosteron sulfata (DHEAS) u plazmi osoba s neurokognitivnim poremećajem [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2023 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:217:651986

N. Balić, "Polimorfizam gena SULT2A1 i koncentracija dehidroepiandrosteron sulfata (DHEAS) u plazmi osoba s neurokognitivnim poremećajem", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:217:651986

Metadata
TitlePolimorfizam gena SULT2A1 i koncentracija dehidroepiandrosteron sulfata (DHEAS) u plazmi osoba s neurokognitivnim poremećajem
Title (english)Polymorphism of SULT2A1 gene and plasma levels of dehydroepiandrosterone sulfate (DHEAS) in individuals with neurocognitive disorder
AuthorNikola Balić
MentorDubravka Švob Štrac (mentor)
MentorDubravka Hranilović (komentor)
Committee memberDubravka Hranilović (predsjednik povjerenstva)
Committee memberMartina Šeruga Musić (član povjerenstva)
Committee memberJulija Erhardt (član povjerenstva)
Committee memberSilvija Černi (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2023-09-21, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Neurosteroid dehidroepiandrosteron sulfat (DHEAS) uključen je u brojne moždane funkcije, uključujući učenje, pamćenje, ponašanje, neurogenezu, neuralnu plastičnost i neuroprotekciju. Istraživanja su utvrdila povezanost između endogenih koncentracija DHEAS-a i demencije, te kognitivnih sposobnosti osoba tijekom starenja. Međutim, u osoba s demencijom utvrđene su snižene, ali i povišene koncentracije DHEAS-a povezane s kognitivnim disfunkcijama, dok je pozitivan učinak DHEAS-a na kognitivne funkcije primijećen samo u nekim studijama. Cilj rada bio je istražiti razlike u plazmatskoj koncentraciji DHEAS-a te raspodjeli genotipova polimorfizma rs2637125 gena SULT2A1, koji kodira za enzim sulfotransferazu 2A1, odgovornu za pretvorbu dehidroepiandrosterona u sulfatni oblik, u 213 osoba s blagim i 91 osobe s velikim neurokognitivnim poremećajem. Također sam istražio utječu li varijacije u genu SULT2A1 na koncentraciju DHEAS-a u plazmi te postoji li njihova povezanost s težinom neurokognitivnog poremećaja. Dijagnoza demencije postavljena je prema DSM-5 kriterijima, a kognitivni simptomi evaluirani su testovima procjene mentalnog stanja. Iz krvi osoba izolirao sam plazmu i DNA. U plazmi sam odredio koncentraciju DHEAS-a metodom ELISA, dok sam genotipizaciju proveo metodom lančane reakcije polimerazom u stvarnom vremenu. Istraživanje nije utvrdilo značajne razlike u plazmatskoj koncentraciji DHEAS-a između osoba s blagim i velikim neurokognitivnim poremećajem, kao niti značajnu povezanost SULT2A1 polimorfizma s težinom neurokognitivnog poremećaja.
Abstract (english)
Neurosteroid dehydroepiandrosterone sulfate (DHEAS) is involved in numerous brain functions, including learning, memory, behavior, neurogenesis, neural plasticity, and neuroprotection. Research suggested association of endogenous DHEAS concentrations with dementia and cognitive functions during aging. However, in dementia patients, both decreased and increased DHEAS concentrations were associated with cognitive dysfunctions, while DHEAS beneficial effects on cognition were observed only in some studies. The study aim was to investigate differences in DHEAS plasma concentration and genotype distribution of rs2637125 polymorphism in SULT2A1 gene, coding for enzyme sulfotransferase 2A1, responsible for conversion of dehydroepiandrosterone into its sulfate form, in 213 people with mild and 91 people with severe neurocognitive disorder. I also investigated whether variations in SULT2A1 gene affect plasma DHEAS concentration and their association with neurocognitive disorder severity. Dementia was diagnosed according to DSM-5 criteria, and cognitive symptoms were evaluated using mental state examination tests. Plasma and DNA were isolated from blood of subjects. Plasma DHEAS concentration was determined using ELISA method, while genotyping was performed using real time polymerase chain reaction.The study did not find any significant differences in DHEAS plasma concentration between individuals with mild and severe neurocognitive disorder, or any significant association of SULT2A1 polymorphism with severity of neurocognitive disorder.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:651986
ProjectNumber: IP-2019-04-6100
Title: Terapijski potencijal neurosteroida i neurotrofina u demenciji
Title: Therapeutic potential of neurosteroids and neurotrophins in dementia
Acronym: TePoNeDe
Leader: Dubravka Švob Štrac
Jurisdiction: Croatia
Funder: HRZZ
Funding stream: IP
Study programmeTitle: Experimental Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2024-09-21
Terms of useLicence In copyright icon
Created on2023-10-16 10:23:18