prikaz prve stranice dokumenta Razgradnja pogrešno smotanih modelnih proteina tGnd1, stGnd1 i NBD2* u kvascu Saccharomyces cerevisiae na podlozi siromašnoj glukozom
Embargo period 2025-09-27
master's thesis
Razgradnja pogrešno smotanih modelnih proteina tGnd1, stGnd1 i NBD2* u kvascu Saccharomyces cerevisiae na podlozi siromašnoj glukozom
Zagreb: University of Zagreb, Faculty of Science, 2023. urn:nbn:hr:217:328937
Guzalić, Dominik
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Guzalić, D. (2023). Razgradnja pogrešno smotanih modelnih proteina tGnd1, stGnd1 i NBD2* u kvascu Saccharomyces cerevisiae na podlozi siromašnoj glukozom (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:328937

Guzalić, Dominik. "Razgradnja pogrešno smotanih modelnih proteina tGnd1, stGnd1 i NBD2* u kvascu Saccharomyces cerevisiae na podlozi siromašnoj glukozom." Master's thesis, University of Zagreb, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:217:328937

Guzalić, Dominik. "Razgradnja pogrešno smotanih modelnih proteina tGnd1, stGnd1 i NBD2* u kvascu Saccharomyces cerevisiae na podlozi siromašnoj glukozom." Master's thesis, University of Zagreb, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:217:328937

Guzalić, D. (2023). 'Razgradnja pogrešno smotanih modelnih proteina tGnd1, stGnd1 i NBD2* u kvascu Saccharomyces cerevisiae na podlozi siromašnoj glukozom', Master's thesis, University of Zagreb, Faculty of Science, accessed 03 May 2024, https://urn.nsk.hr/urn:nbn:hr:217:328937

Guzalić D. Razgradnja pogrešno smotanih modelnih proteina tGnd1, stGnd1 i NBD2* u kvascu Saccharomyces cerevisiae na podlozi siromašnoj glukozom [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2023 [cited 2024 May 03] Available at: https://urn.nsk.hr/urn:nbn:hr:217:328937

D. Guzalić, "Razgradnja pogrešno smotanih modelnih proteina tGnd1, stGnd1 i NBD2* u kvascu Saccharomyces cerevisiae na podlozi siromašnoj glukozom", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:217:328937

Metadata
TitleRazgradnja pogrešno smotanih modelnih proteina tGnd1, stGnd1 i NBD2* u kvascu Saccharomyces cerevisiae na podlozi siromašnoj glukozom
Title (english)Degradation of model misfolded proteins tGnd1, stGnd1 and NBD2* in glucose-starved yeast Saccharomyces cerevisiae
AuthorDominik Guzalić
MentorMirta Boban (mentor)
MentorInga Urlić (komentor)
Committee memberInga Urlić (predsjednik povjerenstva)
Committee memberSandra Hudina (član povjerenstva)
Committee memberMaja Matulić (član povjerenstva)
Committee memberSunčica Bosak (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2023-09-27, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Homeostaza proteina ključna je za funkcioniranje svih staničnih procesa. Jedan od osnovnih načina održanja homeostaze proteina je razgradnja proteina sustavom ubikvitin-proteasoma, te procesom autofagije. Dosad provedena istraživanja na stanicama kvasca Saccharomyes cervisiae uzgajanim na mediju bogatom glukozom pokazala su da se modelni pogrešno smotani proteini NBD2*, tGnd1 i stGnd1 razgrađuju selektivno sustavom ubikvitin-proteasoma. Cilj ovog rada bio je ispitati dolazi li u stanicama kvasca u uvjetima akutnog uskraćivanja glukoze do selektivne razgradnje pogrešno smotanog modelnog proteina NBD2*. Osim toga, budući da je poznato kako gladovanje inducira autofagiju, drugi cilj bio je ispitati je li za razgradnju pogrešno smotanih proteina tGnd1 i stGnd1 odgovorna autofagija. Rezultati su pokazali da se modelni pogrešno smotani protein NBD2* u uvjetima akutnog uskraćivanja glukoze razgrađuje selektivno. Nadalje, rezultati pokazuju kako se pogrešno smotani protein stGnd1 razgrađuje molekulskim putem neovisnim o autofagiji, za razliku od pogrešno smotanog proteina tGnd1 koji se u navedenim uvjetima razgrađivao autofagijom. Dobiveni rezultati pokazuju kako je moguće da se različiti pogrešno smotani proteini u uvjetima akutnog uskraćivanja glukoze u stanicama kvasca razgrađuju različitim molekulskim putevima.
Abstract (english)
Protein homeostasis is crucial for the functioning of all cellular processes. One of the fundamental mechanisms for maintaining protein homeostasis is protein degradation through the ubiquitinproteasome system and the process of autophagy. Previous research conducted on Saccharomyces cerevisiae yeast cells cultured in glucose-rich media has shown that the model misfolded proteins NBD2*, tGnd1, and stGnd1 are selectively degraded by the ubiquitin-proteasome system. The aim of this study was to investigate whether selective degradation of the model misfolded protein NBD2* occurs in yeast cells under conditions of acute glucose deprivation. Additionally, since it is known that starvation induces autophagy, another goal was to examine whether autophagy is responsible for the degradation of misfolded proteins tGnd1 and stGnd1. The results demonstrated that the model misfolded protein NBD2* is selectively degraded under conditions of acute glucose deprivation. Furthermore, the results indicate that the misfolded protein stGnd1 is degraded through a molecular pathway independent of autophagy, in contrast to the misfolded protein tGnd1 which was degraded by autophagy under the mentioned conditions. The obtained results suggest that different misfolded proteins may be degraded through distinct molecular pathways in yeast cells during acute glucose deprivation.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:328937
ProjectNumber: PZS-2019-02-3610
Title: Kontrola kvalitete proteina putem selektivne razgradnje u stanicama u mirovanju
Title: Degradation-mediated protein quality control in quiescent cells
Acronym: Q-CTRL
Leader: Mirta Boban
Jurisdiction: Croatia
Funder: HRZZ
Funding stream: Nema
ProjectNumber: KK.01.1.1.01.0007
Title: Eksperimentalna i klinička istraživanja hipoksijsko-ishemijskog oštećenja mozga u perinatalnoj i odrasloj dobi
Leader: Ivica Kostović
Jurisdiction: Croatia
Funder: EK
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsEmbargoed access
Embargo expiration date: 2025-09-27
Terms of useLicence In copyright icon
Created on2023-10-17 11:04:39