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master's thesis
Procjena tolerancije varijeteta divljeg kupusa (Brassica incana) na čimbenike abiotičkog stresa
Zagreb: University of Zagreb, Faculty of Science, 2023. urn:nbn:hr:217:501765
Baotić, Krunoslav
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Baotić, K. (2023). Procjena tolerancije varijeteta divljeg kupusa (Brassica incana) na čimbenike abiotičkog stresa (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:501765

Baotić, Krunoslav. "Procjena tolerancije varijeteta divljeg kupusa (Brassica incana) na čimbenike abiotičkog stresa." Master's thesis, University of Zagreb, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:217:501765

Baotić, Krunoslav. "Procjena tolerancije varijeteta divljeg kupusa (Brassica incana) na čimbenike abiotičkog stresa." Master's thesis, University of Zagreb, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:217:501765

Baotić, K. (2023). 'Procjena tolerancije varijeteta divljeg kupusa (Brassica incana) na čimbenike abiotičkog stresa', Master's thesis, University of Zagreb, Faculty of Science, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:501765

Baotić K. Procjena tolerancije varijeteta divljeg kupusa (Brassica incana) na čimbenike abiotičkog stresa [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2023 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:217:501765

K. Baotić, "Procjena tolerancije varijeteta divljeg kupusa (Brassica incana) na čimbenike abiotičkog stresa", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:217:501765

Metadata
TitleProcjena tolerancije varijeteta divljeg kupusa (Brassica incana) na čimbenike abiotičkog stresa
Title (english)Evaluation of wild cabbage (Brassica incana) varieties tolerance to abiotic stress factors
AuthorKrunoslav Baotić
MentorBranka Salopek Sondi (mentor)
MentorMartina Šeruga Musić (komentor)
Committee memberMartina Šeruga Musić (predsjednik povjerenstva)
Committee memberSandra Radić Brkanac (član povjerenstva)
Committee memberSunčica Bosak (član povjerenstva)
Committee memberMaja Matulić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2023-09-22, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Klimatske promjene i s njima često povezan abiotički stres imaju vrlo značajan utjecaj na rast i razvoj biljaka. Za suočavanje s nepovoljnim uvjetima u okolišu biljke koriste različite strategije i mehanizme kako bi smanjile njihov negativan učinak. U ovom istraţivanju ispitano je šest varijeteta divljih kupusa (Brassica incana) koji prirodno rastu na liticama hrvatskih otoka u izuzetno nepovoljnim uvjetima. Klijanci uzgajani na 1 %-tnom agaru bili su podvrgnuti solnom (100 mM NaCl), osmotskom (200 mM manitol) i temperaturnom (maksimum na 40 °C) stresu, te kombinaciji navedenih vrsta stresova. Dobiveni rezultati usporeĎeni su s tri varijeteta, morfološki vrlo slične, raštike (Brassica acephala var. capitata) koji su tretirani na isti način kao i divlji kupus. Odgovor biljaka na stres pratio sam testom inhibicije rasta te mjerenjem biokemijskih markera stresa. Na osnovu dobivenih rezultata odabrao sam dva varijeteta divljeg kupusa i dva varijeteta raštike na kojima sam dodatno, pomoću metode qPCR, pratio ekspresiju gena (NAC041, NAC084 i DREB2A) uključenih u odgovoru biljaka na stres. Temperaturni tretman je kod mjerenja biomase imao najmanji utjecaj na rast i razvoj, dok je najveće smanjenje uzrokovao osmotski stres. Kod divljeg kupusa je na svim tretmanima, a posebno na solnom i osmotskom, zapaţeno povećanje sadrţaja prolina. Različiti varijeteti divljih kupusa pokazali su različite antioksidativne odgovore na svakom tretmanu, dok se razina malondialdehida (MDA) u najviše slučajeva povećavala kod solnog stresa. Povećana akumulacija ukupnih fenola u divljih kupusa zabiljeţena je kod solnog i osmotskog stresa. Tretmani koji su uključivali temperaturu su u najvećem broju slučajeva uzrokovali povećanu ekspresiju gena NAC, a ekspresija gena DREB2A bila je zabiljeţena na svim tretmanima što opravdava njegovu ulogu u otpornosti na stres.
Abstract (english)
Climate changes and the often associated abiotic stress have a very significant impact on the growth and development of plants. To cope with adverse environmental conditions, plants use different strategies and mechanisms to reduce their impact. In this research, six varieties of wild cabbage (Brassica incana) that grow naturally on the cliffs of Croatian islands in extremely unfavorable conditions were examined. Seedlings grown on 1% agar were subjected to salt (100 mM NaCl), osmotic (200 mM mannitol) and temperature (maximum at 40 °C) stress, as well as a combination of these types of stress. The obtained results were compared with three varieties, morphologically very similar, kale (Brassica acephala var. capitata) which were treated in the same way as wild cabbage. I monitored the plants response to stress with a growth inhibition test and by measuring biochemical markers of stress. Based on the obtained results, I selected two varieties of wild cabbage and two varieties of kale on which I additionally monitored the expression of the stress responsive genes (NAC041, NAC084 and DREB2A) by using PCR. Temperature treatment had the least impact on biomass measurement, while osmotic stress caused the strongest reduction. In wild cabbage, an increase in proline content was observed in all treatments, particularly salinity and osmotic stress. Different varieties of wild cabbage showed different antioxidative responses to each treatment, while the level of malondialdehyde (MDA) increased in most cases under salt stress. Increased accumulation of total phenols in wild cabbage was recorded under salt and osmotic stress. Treatments that included temperature caused increased NAC gene expression in most cases, and DREB2A gene expression was recorded in all treatments, which justifies its role in stress resistance.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:501765
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2024-09-22
Terms of useLicence In copyright icon
Created on2023-10-17 11:48:26