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master's thesis
Utjecaj sustava izbacivanja emrBAR na simbiozu biljke Medicago truncatula i bakterije Sinorhizobium meliloti
Zagreb: University of Zagreb, Faculty of Science, 2016. urn:nbn:hr:217:115786
Mitić, Damjan
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Mitić, D. (2016). Utjecaj sustava izbacivanja emrBAR na simbiozu biljke Medicago truncatula i bakterije Sinorhizobium meliloti (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:115786

Mitić, Damjan. "Utjecaj sustava izbacivanja emrBAR na simbiozu biljke Medicago truncatula i bakterije Sinorhizobium meliloti." Master's thesis, University of Zagreb, Faculty of Science, 2016. https://urn.nsk.hr/urn:nbn:hr:217:115786

Mitić, Damjan. "Utjecaj sustava izbacivanja emrBAR na simbiozu biljke Medicago truncatula i bakterije Sinorhizobium meliloti." Master's thesis, University of Zagreb, Faculty of Science, 2016. https://urn.nsk.hr/urn:nbn:hr:217:115786

Mitić, D. (2016). 'Utjecaj sustava izbacivanja emrBAR na simbiozu biljke Medicago truncatula i bakterije Sinorhizobium meliloti', Master's thesis, University of Zagreb, Faculty of Science, accessed 25 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:115786

Mitić D. Utjecaj sustava izbacivanja emrBAR na simbiozu biljke Medicago truncatula i bakterije Sinorhizobium meliloti [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2016 [cited 2024 November 25] Available at: https://urn.nsk.hr/urn:nbn:hr:217:115786

D. Mitić, "Utjecaj sustava izbacivanja emrBAR na simbiozu biljke Medicago truncatula i bakterije Sinorhizobium meliloti", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:217:115786

Metadata
TitleUtjecaj sustava izbacivanja emrBAR na simbiozu biljke Medicago truncatula i bakterije Sinorhizobium meliloti
Title (english)The influence of emrBAR efflux system on symbiosis between Medicago truncatula and Sinorhizobium meliloti
AuthorDamjan Mitić
MentorMichael Göttfert (mentor)
MentorIvana Ivančić Baće (mentor)
Committee memberIvana Ivančić Baće (predsjednik povjerenstva)
Committee memberBranka Pevalek-Kozlina (član povjerenstva)
Committee memberGoran Kovačević (član povjerenstva)
Committee memberŽeljka Vidaković-Cifrek (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2016-02-29, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Dušik je bitan element biomolekula i potreban je za metabolizam i razvoj svih biljaka. Simbiotske interakcije između bakterija i biljaka razvile su se kako bi se omogućila apsorpcija dušika u biljkama. Glavni cilj ovog istraživanja bio je učinak sustava izbacivanja EmrBAR na simbiozu mahunarke Medicago truncatula i bakterije Sinorhizobium meliloti. Rezultati su pokazali da su geni emrA i emrR eksprimirani u ranim fazama simbiotskih interakcija, tijekom nastajanja infekcijskih niti. Gen emrA je eksprimiran u infekcijskoj zoni i zoni fiksacije dušika, a gen emrR samo u infekcijskoj zoni korijenove kvržice. Analiza nodulacijskog fenotipa emrBAR mutanata i divljeg tipa u nakošenim epruvetama ispunjenim agarom sugerira da je stvaranje novih korijenovih kvržica bio odgovor na neučinkovitu fiksaciju dušika. Analiza nodulacijskog fenotipa emrBAR mutanata i divljeg tipa u Magenta posudama sugerira da delecija emrBAR utječe na rast simbiontskih biljaka smanjujući učinkovitost fiksacije dušika u korijenovim kvržicama. Ispitivanje rasta bakterija je pokazalo da 2-fenilfenol inhibira rast dvaju sojeva emrBAR mutanta u odnosu na divlji tip. To bi moglo ukazivati na važnost sustava izbacivanja EmrBAR u izbacivanju otrovnih tvari iz bakterijskih stanica.
Abstract (english)
Nitrogen is an essential part of biomolecules and is needed for the metabolism and development of all plants. Symbiotic interactions between bacteria and plants have evolved in order to enable the absorption of nitrogen to plants. The main focus of this study was the effect of the EmrBAR efflux system on the symbiosis between the legume Medicago truncatula and the rhizobium Sinorhizobium meliloti. The results showed that emrA and emrR are expressed in early symbiotic interactions, during the formation of infection threads. EmrA is expressed in the infection and nitrogen-fixation zones, and emrR only in the infection zone of the nodule. Analysis of nodulation phenotype of the emrBAR deletion mutants and the wild type in sloped agar tubes might suggest that the creation of new nodules was a response to inefficient nitrogen fixation. Analysis of nodulation phenotype of the emrBAR deletion mutants and the wild type in Magenta boxes suggests that the emrBAR deletion affects symbiotic plant growth by decreasing nodule efficiency. A bacterial growth assay showed that 2-phenylphenol inhibits the growth of the two emrBAR deletion mutant strains in comparison with the wild type. This seems to indicate the importance of the EmrBAR efflux system in the extrusion of toxic substances.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:115786
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-05-08 11:12:19