prikaz prve stranice dokumenta Uloga estrogena i receptora estrogena u metastatskom potencijalu tumora glave i vrata
Embargo period 2025-09-29
master's thesis
Uloga estrogena i receptora estrogena u metastatskom potencijalu tumora glave i vrata
Zagreb: University of Zagreb, Faculty of Science, 2023. urn:nbn:hr:217:160036
Kranjčević, Jacqueline-Katrin
University of Zagreb
Faculty of Science
Department of Chemistry

Institutional repository: Repository of the Faculty of Science

Cite this document

Kranjčević, J. K. (2023). Uloga estrogena i receptora estrogena u metastatskom potencijalu tumora glave i vrata (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:160036

Kranjčević, Jacqueline Katrin. "Uloga estrogena i receptora estrogena u metastatskom potencijalu tumora glave i vrata." Master's thesis, University of Zagreb, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:217:160036

Kranjčević, Jacqueline Katrin. "Uloga estrogena i receptora estrogena u metastatskom potencijalu tumora glave i vrata." Master's thesis, University of Zagreb, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:217:160036

Kranjčević, J. K. (2023). 'Uloga estrogena i receptora estrogena u metastatskom potencijalu tumora glave i vrata', Master's thesis, University of Zagreb, Faculty of Science, accessed 09 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:160036

Kranjčević JK. Uloga estrogena i receptora estrogena u metastatskom potencijalu tumora glave i vrata [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2023 [cited 2024 November 09] Available at: https://urn.nsk.hr/urn:nbn:hr:217:160036

J. K. Kranjčević, "Uloga estrogena i receptora estrogena u metastatskom potencijalu tumora glave i vrata", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:217:160036

Metadata
TitleUloga estrogena i receptora estrogena u metastatskom potencijalu tumora glave i vrata
Title (english)The role of estrogen and estrogen receptors in the metastatic potential of head and neck tumors
AuthorJacqueline-Katrin Kranjčević
MentorPetar Ozretić (mentor)
Committee memberJasmina Rokov Plavec (predsjednik povjerenstva)
Committee memberIva Juranović Cindrić (član povjerenstva)
Committee memberMirta Rubčić (član povjerenstva)
Committee memberMorana Dulić (član povjerenstva)
Committee memberIvica Đilović (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Chemistry)
Zagreb
Defense date and country2023-09-29, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
Muškarci imaju dva do četiri puta veći rizik od razvijanja tumora glave i vrata (TGV) od žena, neovisno o etiološkom agensu. Stoga se smatra da izloženost estrogenu ženama pruža zaštitu od nastanka i metastaziranja TGV. Estrogeni svoju funkciju ostvaruju vezanjem za receptore estrogena, koji se mogu podijeliti na jezgrene (ERα i ERβ) i membranske receptore estrogena (GPER1 i NaV1.2). Vezanjem estrogena dolazi do aktivacije transkripcije određenih gena i/ili signalnih kaskada, koje naposlijetku utječu na gensku ekspresiju. Metodom RT-qPCR određen je ekspresijski profil gena za receptore estrogena u uzorcima zdrave sluznice usne šupljine, primarnih tumora i metastatskih limfnih čvorova te u staničnoj liniji Detroit 562 nakon tretmana 17β-estradiolom. Pomoću testa zacjeljivanja rana provjerena je migracijska sposobnost stanica nakon tretmana 17β-estradiolom i tamoksifenom. Rezultati su pokazali da bi ERα i ERβ mogli imati ulogu u razvoju TGV, ali da tamoksifen vjerojatno nije pogodan oblik liječenja TGV.
Abstract (english)
Men are at a two- to fourfold higher risk of developing head and neck cancers (HNC) than women, regardless of aetiological agent. Therefore, estrogen is considered to provide protection from HNC development and metastasis in women. Estrogens exert their function by binding to estrogen receptors, which can be divided into nuclear (ERα and ERβ) and membrane estrogen receptors (GPER1 and NaV1.2). Estrogen binding activates the transcription of certain genes and/or signaling cascades, which ultimately regulate gene expression. RT-qPCR was used to determine the expression profile of estrogen receptor genes in healthy mucosa, primary tumor and metastatic lymph node samples as well as the cell line Detroit 562 after 17β-estradiol treatment. The wound healing assay was used to study cell migration after 17β-estradiol and tamoxifen treatment. Results showed that ERα and ERβ might be involved in HNC development, but tamoxifen probably isn't a suitable treatment option for HNC.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:160036
Study programmeTitle: Graduate university studies in chemistry; specializations in: Research programme
Course: Research programme
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
Type of resourceText
File originBorn digital
Access conditionsEmbargoed access
Embargo expiration date: 2025-09-29
Terms of useLicence In copyright icon
Created on2023-10-19 13:58:27