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doctoral thesis
Utjecaj polimorfizama TLR5 (rs2072493) i TLR7 (rs5935436) na transkriptom i metabolom u staničnom modelu karcinoma pluća nemalih stanica
Zagreb: University of Zagreb, Faculty of Science, 2023. urn:nbn:hr:217:259171
Baranašić, Jurica
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Baranašić, J. (2023). Utjecaj polimorfizama TLR5 (rs2072493) i TLR7 (rs5935436) na transkriptom i metabolom u staničnom modelu karcinoma pluća nemalih stanica (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:259171

Baranašić, Jurica. "Utjecaj polimorfizama TLR5 (rs2072493) i TLR7 (rs5935436) na transkriptom i metabolom u staničnom modelu karcinoma pluća nemalih stanica." Doctoral thesis, University of Zagreb, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:217:259171

Baranašić, Jurica. "Utjecaj polimorfizama TLR5 (rs2072493) i TLR7 (rs5935436) na transkriptom i metabolom u staničnom modelu karcinoma pluća nemalih stanica." Doctoral thesis, University of Zagreb, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:217:259171

Baranašić, J. (2023). 'Utjecaj polimorfizama TLR5 (rs2072493) i TLR7 (rs5935436) na transkriptom i metabolom u staničnom modelu karcinoma pluća nemalih stanica', Doctoral thesis, University of Zagreb, Faculty of Science, accessed 23 December 2024, https://urn.nsk.hr/urn:nbn:hr:217:259171

Baranašić J. Utjecaj polimorfizama TLR5 (rs2072493) i TLR7 (rs5935436) na transkriptom i metabolom u staničnom modelu karcinoma pluća nemalih stanica [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Science; 2023 [cited 2024 December 23] Available at: https://urn.nsk.hr/urn:nbn:hr:217:259171

J. Baranašić, "Utjecaj polimorfizama TLR5 (rs2072493) i TLR7 (rs5935436) na transkriptom i metabolom u staničnom modelu karcinoma pluća nemalih stanica", Doctoral thesis, University of Zagreb, Faculty of Science, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:217:259171

Metadata
TitleUtjecaj polimorfizama TLR5 (rs2072493) i TLR7 (rs5935436) na transkriptom i metabolom u staničnom modelu karcinoma pluća nemalih stanica
Title (english)Influence of TLR5 (rs2072493) and TLR7 (rs5935436) polymorphisms on transcriptome and metabolome of in vitro model of metastatic non-small cell lung carcinoma
AuthorJurica Baranašić
MentorJelena Knežević (mentor)
Committee memberNada Oršolić (predsjednik povjerenstva)
Committee memberPetar Ozretić (član povjerenstva)
Committee memberDragomira Majhen (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2023-10-06, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Universal decimal classification
(UDC)		577 - Biochemistry. Molecular biology. Biophysics
Abstract
Karcinom pluća je jedna od najčešćih i najsmrtonosnijih malignih bolesti u svijetu. Uz izloženost duhanskom dimu, važan čimbenik u nastanku i razvoju navedene bolesti je i genetska podloga. Aktivacijom receptora Toll-like (TLR) pokreće se složeni signalni put koji u konačnici kulminira oslobađanjem različitih transkripcijskih čimbenika. Istraživanja koja su prethodila doktorskoj disertaciji pokazala su povezanost polimorfizma u genima TLR5 (rs2072493) i TLR7 (rs5935436) s nastankom karcinoma pluća. Cilj ovog istraživanja bio je ispitati utjecaj ovih polimorfizama na transkriptom i metabolom u uvjetima in vitro. Rezultati analize transkriptoma pokazuju da polimorfizam rs2072493 utječe na promjene u aktivnosti signalnog puta receptora spregnutim s proteinima G (GPCR). Rezultati analize metaboloma pokazuju da prisutnosti polimorfizma rs2072493 mijenja aktivnosti metabolizma triptofana. Nadalje, povećanjem ekspresije gena TLR7, metastatske stanice karcinoma pluća, H1299, postaju osjetljivije na kotretman imikvimodom i paklitakselom. Također, promjena u ekspresiji TLR7 utječe na promjene u aktivnosti staničnog puta glikolize, signalnog puta HIF-1, i signalnog puta AMPK.
Abstract (english)
Lung cancer is one of the leading causes of cancer-related deaths worldwide. Exposure to smoke is the main cause of developing lung cancer, genetic background also has a significant role. Activation of Toll-like receptors (TLR) starts a complex signaling pathway, which in the end releases transcriptional factors. Previous associations studies linked polymorphisms in TLR5 (rs2072493) and TLR7 (rs5935436) with higher susceptibility of developing lung cancer. The aim of this research was to examine the effect of these polymorphisms on the transcriptome and metabolome in vitro. The results of the transcriptome analysis show that the rs2072493 polymorphism affects changes in the activity of the G protein-coupled receptor (GPCR) signaling pathway. The results of the metabolome analysis show that the presence of the rs2072493 polymorphism changes the activity of tryptophan metabolism. Furthermore, metastatic lung cancer cells, H1299, overexpressing TLR7 are more sensitive to co-treatment with imiquimod and paclitaxel. Also, the change in TLR7 expression affects the activity of glycolysis metabolism, the HIF-1 signaling pathway, and the AMPK signaling pathway.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:259171
Promotion2023
Study programmeTitle: Doctoral study
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti u području prirodnih znanosti (doktor/doktorica znanosti u području prirodnih znanosti)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2024-10-06
Terms of useLicence In copyright icon
Created on2023-10-25 08:06:53