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doctoral thesis
ULOGA EKSPRESIJE RECEPTORA NOTCH1 I LIGANADA JAGGED1 I DLL1 U KRVOTVORNIM MATIČNIM STANICAMA MIKROOKOLIŠA MULTIPLOGA MIJELOMA
Zagreb: University of Zagreb, Faculty of Science, 2023. urn:nbn:hr:217:592012
Jurenec, Silvana
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Jurenec, S. (2023). ULOGA EKSPRESIJE RECEPTORA NOTCH1 I LIGANADA JAGGED1 I DLL1 U KRVOTVORNIM MATIČNIM STANICAMA MIKROOKOLIŠA MULTIPLOGA MIJELOMA (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:592012

Jurenec, Silvana. "ULOGA EKSPRESIJE RECEPTORA NOTCH1 I LIGANADA JAGGED1 I DLL1 U KRVOTVORNIM MATIČNIM STANICAMA MIKROOKOLIŠA MULTIPLOGA MIJELOMA." Doctoral thesis, University of Zagreb, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:217:592012

Jurenec, Silvana. "ULOGA EKSPRESIJE RECEPTORA NOTCH1 I LIGANADA JAGGED1 I DLL1 U KRVOTVORNIM MATIČNIM STANICAMA MIKROOKOLIŠA MULTIPLOGA MIJELOMA." Doctoral thesis, University of Zagreb, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:217:592012

Jurenec, S. (2023). 'ULOGA EKSPRESIJE RECEPTORA NOTCH1 I LIGANADA JAGGED1 I DLL1 U KRVOTVORNIM MATIČNIM STANICAMA MIKROOKOLIŠA MULTIPLOGA MIJELOMA', Doctoral thesis, University of Zagreb, Faculty of Science, accessed 28 April 2024, https://urn.nsk.hr/urn:nbn:hr:217:592012

Jurenec S. ULOGA EKSPRESIJE RECEPTORA NOTCH1 I LIGANADA JAGGED1 I DLL1 U KRVOTVORNIM MATIČNIM STANICAMA MIKROOKOLIŠA MULTIPLOGA MIJELOMA [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Science; 2023 [cited 2024 April 28] Available at: https://urn.nsk.hr/urn:nbn:hr:217:592012

S. Jurenec, "ULOGA EKSPRESIJE RECEPTORA NOTCH1 I LIGANADA JAGGED1 I DLL1 U KRVOTVORNIM MATIČNIM STANICAMA MIKROOKOLIŠA MULTIPLOGA MIJELOMA", Doctoral thesis, University of Zagreb, Faculty of Science, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:217:592012

Metadata
TitleULOGA EKSPRESIJE RECEPTORA NOTCH1 I LIGANADA JAGGED1 I DLL1 U KRVOTVORNIM MATIČNIM STANICAMA MIKROOKOLIŠA MULTIPLOGA MIJELOMA
Title (english)THE ROLE OF NOTCH1 RECEPTOR, JAGGED1 AND DLL1 LIGAND EXPRESSION IN HEMATOPOIETIC STEM CELLS OF THE MICROENVIRONMENT IN MULTIPLE MYELOMA
AuthorSilvana Jurenec
MentorAnita Škrtić (mentor)
MentorMariastefania Antica (komentor)
Committee memberDelfa Radić Krišto (predsjednik povjerenstva)
Committee memberMaja Matulić (član povjerenstva)
Committee memberSanja Kapitanović (član povjerenstva)
Committee memberPetra Korać (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2023-09-29, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Universal decimal classification
(UDC)		577 - Biochemistry. Molecular biology. Biophysics
Abstract
Signalni put Notch u krvotvornim matičnim stanicama (KMS) koštane srži (KS) multiplog mijeloma (MM) analiziran je kroz ekspresiju proteina NOTCH1, JAGGED1, DLL1 u KMS imunofenotipizacijom, u tumorskim plazma stanicama (TPS) imunohistokemijskim bojenjem, uz analizu citogenetičkih promjena visokog rizika (CGVR) u TPS metodom FISH te analizu histoloških promjena u koštanoj srži MM. Ekspresija JAGGED1 i DLL1 u KMS značajno je niža u MM nego u zdravih darivatelja. Prisutna je negativna korelacija između ekspresije NOTCH1 i JAGGED1 u KMS MM, a pozitivna korelacija između ekspresije NOTCH1 i liganada JAGGED1 i DLL1 u TPS. U TPS s prisutnom barem jednom CGVR i difuznim infiltratom KS značajno je veća razina ekspresije NOTCH1 i JAGGED1 i intenziteta ekspresije NOTCH1. Potvrđena je hipoteza da postoji razlika u ekspresiji JAGGED1 i DLL1 u KMS u MM i zdravoj KS, no nije dokazana povezanost ekspresije NOTCH1, JAGGED1, DLL1 u KMS i TPS u MM. Povezanost ekspresije NOTCH1, JAGGED1 i DLL1 u istoj vrsti stanica ukazuje na razliku u homotipnoj i heterotipnoj staničnoj signalizaciji puta Notch u MM.
Abstract (english)
The Notch signaling pathway in hematopoietic stem cells (HSC) in the multiple myeloma (MM) bone marrow (BM) was analyzed with the NOTCH1, JAGGED1, DLL1 expression in HSC by immunophenotyping and in tumor plasma cells (TPC) by immunohistochemical staining. High risk cytogenetic changes (CGHR) using the FISH method and histological analysis of TPC in the BM MM was performed. JAGGED1 and DLL1 expression in HSC were significantly lower in MM than in healthy donors. There was a negative correlation between NOTCH1 and JAGGED1 expression in HSC MM, and a positive correlation between NOTCH1 and JAGGED1 and DLL1 in TPC, respectively. In TPC with at least one CGHR present and a BM diffuse infiltrate, the NOTCH1 and JAGGED1 expression and the intensity of NOTCH1 expression were significantly higher. The hypothesis that there is a difference in the expression of JAGGED1 and DLL1 in HSC between MM and healthy BM was confirmed, but the correlation of the NOTCH1, JAGGED1, DLL1 expression in HSC and TPC in MM was not proven. The association of NOTCH1, JAGGED1 and DLL1 expression in the same cell type indicates a difference in homotypic and heterotypic cellular signaling of the Notch pathway in MM.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:592012
Promotion2023
ProjectNumber: IP-2020-02-2431
Title: Obnova timusa za preciznu medicinu u liječenju tumora i leukemija
Title: Thymus renewal for precision medicine in cancer and leukaemia treatment
Acronym: THYMINNOVA
Leader: Mariastefania Antica
Jurisdiction: Croatia
Funder: HRZZ
Funding stream: IP
Study programmeTitle: Doctoral study
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti u području prirodnih znanosti (doktor/doktorica znanosti u području prirodnih znanosti)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2023-10-25 09:12:39