prikaz prve stranice dokumenta Investigating chromosome segregation errors in a set of human cancer cell lines by lattice light-sheet microscopy
Embargo period 2025-10-19
master's thesis
Investigating chromosome segregation errors in a set of human cancer cell lines by lattice light-sheet microscopy
Zagreb: University of Zagreb, Faculty of Science, 2023. urn:nbn:hr:217:107191
Đura, Mario
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Đura, M. (2023). Investigating chromosome segregation errors in a set of human cancer cell lines by lattice light-sheet microscopy (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:107191

Đura, Mario. "Investigating chromosome segregation errors in a set of human cancer cell lines by lattice light-sheet microscopy." Master's thesis, University of Zagreb, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:217:107191

Đura, Mario. "Investigating chromosome segregation errors in a set of human cancer cell lines by lattice light-sheet microscopy." Master's thesis, University of Zagreb, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:217:107191

Đura, M. (2023). 'Investigating chromosome segregation errors in a set of human cancer cell lines by lattice light-sheet microscopy', Master's thesis, University of Zagreb, Faculty of Science, accessed 25 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:107191

Đura M. Investigating chromosome segregation errors in a set of human cancer cell lines by lattice light-sheet microscopy [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2023 [cited 2024 November 25] Available at: https://urn.nsk.hr/urn:nbn:hr:217:107191

M. Đura, "Investigating chromosome segregation errors in a set of human cancer cell lines by lattice light-sheet microscopy", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:217:107191

Metadata
TitleInvestigating chromosome segregation errors in a set of human cancer cell lines by lattice light-sheet microscopy
Title (croatian)Istraživanje pogrešaka segregacije kromosoma u skupu ljudskih staničnih linija raka pomoću mikroskopa s rešetkastom svjetlosnom plohom
AuthorMario Đura
MentorIva Marija Tolić (mentor)
Committee memberIva Marija Tolić (predsjednik povjerenstva)
Committee memberMaja Matulić (član povjerenstva)
Committee memberPetra Peharec Štefanić (član povjerenstva)
Committee memberNada Oršolić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2023-10-19, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Chromosomal instability is a hallmark of cancer cells that furthers tumour progression by the mis-segregation of chromosomes. Even though certain aspects of it have long been described, novel phenomena are being established as major predictors of mitotic errors in cancers with chromosomal instability. In this thesis, chromosomal instability was measured on 10 various cancer cell lines through the occurrence of metaphase unaligned chromosomes, lagging chromosomes, chromosome bridges, multipolar mitoses, micronuclei, transient multipolar transition and other phenomena. Additionally, the co-occurrences of these phenomena were investigated to gain insight into their correlations. The lattice light-sheet microscope allowed for long-term live imaging of these cell lines in a substantial number of observed mitoses, enabling the creation of a database of mitotic errors and phenomena for all cell lines in a timely manner.
Abstract (croatian)
Kromosomska nestabilnost je značajka stanica raka koja potiče progresiju tumora pomoću nepravilne segregacije kromosoma. Iako su njeni pojedini aspekti već davno opisani, novi fenomeni se ukazuju kao snažni čimbenici za predviđanje pogrešaka u mitozi stanica raka koje posjeduju kromosomsku nestabilnost. U ovome radu, kromosomska nestabilnost je izmjerena u 10 raznovrsnih staničnih linija raka kroz pojavnost metafaznih neporavnatih kromosoma, zaostajućih kromosoma, kromosomskih mostova, multipolarnih mitoza, mikronukleusa, tranzijentne multipolarne tranzicije i ostale fenomene. Dodatno, istovremene pojavnosti ovih fenomena su istražene kako bi se dobio uvid u njihove korelacije. Mikroskop s rešetkastom svjetlosnom plohom je omogućio dugotrajno uživo snimanje ovih staničnih linija u značajnom broju promatranih mitoza, što je dozvolilo relativno brzo stvaranje baze podataka mitotskih pogrešaka i fenomena za sve stanične linije.
Keywords
Keywords (croatian)
Languageenglish
URN:NBNurn:nbn:hr:217:107191
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsEmbargoed access
Embargo expiration date: 2025-10-19
Terms of useLicence In copyright icon
Created on2023-10-25 10:25:42