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doctoral thesis
KOMPARATIVNA ANALIZA MOLEKULARNIH BILJEGA TERATOKARCINOMA MIŠA I ČOVJEKA
Zagreb: University of Zagreb, Faculty of Science, 2023. urn:nbn:hr:217:002948
Buljubašić, Maja
University of Zagreb
Faculty of Science

Institutional repository: Repository of the Faculty of Science

Cite this document

Buljubašić, M. (2023). KOMPARATIVNA ANALIZA MOLEKULARNIH BILJEGA TERATOKARCINOMA MIŠA I ČOVJEKA (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:002948

Buljubašić, Maja. "KOMPARATIVNA ANALIZA MOLEKULARNIH BILJEGA TERATOKARCINOMA MIŠA I ČOVJEKA." Doctoral thesis, University of Zagreb, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:217:002948

Buljubašić, Maja. "KOMPARATIVNA ANALIZA MOLEKULARNIH BILJEGA TERATOKARCINOMA MIŠA I ČOVJEKA." Doctoral thesis, University of Zagreb, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:217:002948

Buljubašić, M. (2023). 'KOMPARATIVNA ANALIZA MOLEKULARNIH BILJEGA TERATOKARCINOMA MIŠA I ČOVJEKA', Doctoral thesis, University of Zagreb, Faculty of Science, accessed 28 April 2024, https://urn.nsk.hr/urn:nbn:hr:217:002948

Buljubašić M. KOMPARATIVNA ANALIZA MOLEKULARNIH BILJEGA TERATOKARCINOMA MIŠA I ČOVJEKA [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Science; 2023 [cited 2024 April 28] Available at: https://urn.nsk.hr/urn:nbn:hr:217:002948

M. Buljubašić, "KOMPARATIVNA ANALIZA MOLEKULARNIH BILJEGA TERATOKARCINOMA MIŠA I ČOVJEKA", Doctoral thesis, University of Zagreb, Faculty of Science, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:217:002948

Metadata
TitleKOMPARATIVNA ANALIZA MOLEKULARNIH BILJEGA TERATOKARCINOMA MIŠA I ČOVJEKA
Title (english)COMPARATIVE ANALYSIS OF MOLECULAR MARKERS IN TERATOCARCINOMA OF MICE AND MAN
AuthorMaja Buljubašić
MentorNino Sinčić (mentor)
MentorMonika Ulamec (komentor)
Committee memberDamjan Franjević (predsjednik povjerenstva)
Committee memberAna Katušić Bojanac (član povjerenstva)
Committee memberDavor Ježek (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
Zagreb
Defense date and country2023, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Universal decimal classification
(UDC)		577 - Biochemistry. Molecular biology. Biophysics
Abstract
Etiologija i patogeneza tumora testisa čovjeka ostaju do danas nerazjašnjene. Ograničenom razumijevanju doprinosi i tendencija istraživanja primarno na humanom materijalu te in vitro sustavima. Eksperimentalni teratokarcinom miša jedan je od priznatijih animalnih modela za istraživanje tumora testisa. Međutim, opravdanost korištenja ovog modela temelji se isključivo na morfološkom profiliranju. Cilj ovog istraživanja bio je po prvi puta na molekularnoj razini sustavno analizirati i usporediti mišji model s humanim teratokarcinom, te testirati opravdanost upotrebe ovog animalnog modela. U tu svrhu analizirani su biljezi proliferacije, apoptoze, diferencijacije i matičnosti metodama imunohistokemije i Western blottinga. Rezultati ovog istraživanja ukazuju na to da animalni model eksperimentalnog teratokarcinoma miša vjerno modelira teratokarcinom čovjeka ne samo na morfološkoj već i na molekularno biološkoj razini. Iz svega navedenog proizlazi da je eksperimentalni teratokarcinom miša pogodan za potrebe istraživanja razvoja teratokarcinoma čovjeka na molekularnoj razini, te da je njegova upotreba u znanstvenim istraživanjima opravdana.
Abstract (english)
The etiology and pathogenesis of human testicular tumors remain unclear to this day, in part due to the trend of human material and in vitro systems research. Experimental mouse teratocarcinoma is one of the recognized animal models for testicular tumor research. However, the justification for using this model is based solely on the morphological profile. The goal of this research was to systematically analyze and compare the mouse model with human teratocarcinoma at the molecular level for the first time, and to test the justification of its use in research. For this purpose, markers of proliferation, apoptosis, differentiation and stemness were analyzed using immunohistochemistry and Western blotting. The results indicate that the animal model of experimental mouse teratocarcinoma faithfully models human teratocarcinoma not only at the morphological but also at the molecular level. From all of the above, it follows that experimental mouse teratocarcinoma is suitable for researching the development of human teratocarcinoma at the molecular level, and that its use in scientific research is justified.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:002948
Promotion2023
ProjectNumber: KK.01.1.1.01.0008
Title: Reproduktivna i regenerativna medicina - istraživanje novih platformi i potencijala
Leader: Ana Katušić Bojanac
Jurisdiction: Croatia
Funder: MZO
Study programmeTitle: Doctoral study
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti u području prirodnih znanosti (doktor/doktorica znanosti u području prirodnih znanosti)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2023-10-26 12:26:35