prikaz prve stranice dokumenta SEROLOŠKE I MOLEKULARNE ZNAČAJKE ALOIMUNIZACIJE NA SPECIFIČNE NEUTROFILNE ANTIGENE KOD ALOIMUNE NEONATALNE NEUTROPENIJE
Embargo period 2024-09-21
doctoral thesis
SEROLOŠKE I MOLEKULARNE ZNAČAJKE ALOIMUNIZACIJE NA SPECIFIČNE NEUTROFILNE ANTIGENE KOD ALOIMUNE NEONATALNE NEUTROPENIJE
Zagreb: University of Zagreb, Faculty of Science, 2023. urn:nbn:hr:217:034858
Štimac, Ružica
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Štimac, R. (2023). SEROLOŠKE I MOLEKULARNE ZNAČAJKE ALOIMUNIZACIJE NA SPECIFIČNE NEUTROFILNE ANTIGENE KOD ALOIMUNE NEONATALNE NEUTROPENIJE (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:034858

Štimac, Ružica. "SEROLOŠKE I MOLEKULARNE ZNAČAJKE ALOIMUNIZACIJE NA SPECIFIČNE NEUTROFILNE ANTIGENE KOD ALOIMUNE NEONATALNE NEUTROPENIJE." Doctoral thesis, University of Zagreb, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:217:034858

Štimac, Ružica. "SEROLOŠKE I MOLEKULARNE ZNAČAJKE ALOIMUNIZACIJE NA SPECIFIČNE NEUTROFILNE ANTIGENE KOD ALOIMUNE NEONATALNE NEUTROPENIJE." Doctoral thesis, University of Zagreb, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:217:034858

Štimac, R. (2023). 'SEROLOŠKE I MOLEKULARNE ZNAČAJKE ALOIMUNIZACIJE NA SPECIFIČNE NEUTROFILNE ANTIGENE KOD ALOIMUNE NEONATALNE NEUTROPENIJE', Doctoral thesis, University of Zagreb, Faculty of Science, accessed 07 May 2024, https://urn.nsk.hr/urn:nbn:hr:217:034858

Štimac R. SEROLOŠKE I MOLEKULARNE ZNAČAJKE ALOIMUNIZACIJE NA SPECIFIČNE NEUTROFILNE ANTIGENE KOD ALOIMUNE NEONATALNE NEUTROPENIJE [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Science; 2023 [cited 2024 May 07] Available at: https://urn.nsk.hr/urn:nbn:hr:217:034858

R. Štimac, "SEROLOŠKE I MOLEKULARNE ZNAČAJKE ALOIMUNIZACIJE NA SPECIFIČNE NEUTROFILNE ANTIGENE KOD ALOIMUNE NEONATALNE NEUTROPENIJE", Doctoral thesis, University of Zagreb, Faculty of Science, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:217:034858

Metadata
TitleSEROLOŠKE I MOLEKULARNE ZNAČAJKE ALOIMUNIZACIJE NA SPECIFIČNE NEUTROFILNE ANTIGENE KOD ALOIMUNE NEONATALNE NEUTROPENIJE
Title (english)SEROLOGICAL AND MOLECULAR CHARACTERISTICS OF ALLOIMMUNISATION TO SPECIFIC NEUTROPHIL ANTIGENS IN ALLOIMMUNE NEONATAL NEUTROPENIA
AuthorRužica Štimac
MentorMaja Tomičić (mentor)
Committee memberJasna Bingulac-Popović (predsjednik povjerenstva)
Committee memberMirta Starčević (član povjerenstva)
Committee memberHaidi Arbanasić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2023-09-21, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Universal decimal classification
(UDC)		577 - Biochemistry. Molecular biology. Biophysics
Abstract
Antineutrofilna (anti-HNA) aloantitijela nastaju nakon izlaganja stranim neutrofilnim aloantigenima. Aloimuna neonatalna neutropenija (ANN) nastaje imunizacijom majke tijekom trudnoće specifičnim humanim neutrofilnim antigenima (HNA) djeteta naslijeđenim od oca. U oko trećine slučajeva ANN nije moguće odrediti specifičnost antitijela HNA. U ovom istraživanju svih 10 slučajeva ANN bilo je uzrokovano antitijelima na HNA-1 sustav. U 7 slučajeva dokazana je anti-FcγRIIIb specifičnost antitijela. U 9 slučajeva imunizacija majke bila je uzrokovana aloantitijelima nastalim nepodudarnošću s antigenom HNA-1b djeteta. Viša vrijednost titra antitijela anti-HNA u djece bila je povezana s većim stupnjem neutropenije. Prisutnost antitijela anti-HLA razreda I u majki ima statistički značajan utjecaj na kraće trajanje neutropenije u djece. Ovim istraživanjem potvrđena je hipoteza da primjena osjetljive serološke metode za dokazivanje prisutnosti antitijela anti-HNA i anti-HLA razreda I, određivanje specifičnosti antitijela anti-HNA u plazmi majke i djeteta te molekularne metode za određivanje genotipa HNA majke i djeteta znatno doprinose pravodobnom otkrivanju ove bolesti
Abstract (english)
Antineutrophil (anti-HNA) alloantibodies are produced in response to exposure to foreign neutrophil alloantigens. Neonatal alloimmune neutropenia (NAN) develops due to maternal immunization during pregnancy against neonatal specific human neutrophil antigens (HNA) inherited from the father. HNA antibody specificity cannot be identified in about one-third of NAN cases. In this study, all 10 NAN cases were caused by HNA-1 system antibodies. AntiFcyRIIIb antibody specificity was demonstrated in 7 cases. In 9 cases, maternal immunization was caused by alloantibodies due to incompatibility with neonatal HNA-1b antigen. Elevated anti-HNA antibody titer in children was associated with a higher degree of neutropenia. The presence of class I anti-HLA antibodies in mothers has a statistically significant effect on a shorter duration of neutropenia in children. This study has confirmed the hypothesis that using a sensitive serological method to demonstrate the presence of anti-HNA and class I anti-HLA antibodies, determination of anti-HNA antibody specificity in maternal and neonatal plasma, and employing molecular methods to determine maternal and neonatal HNA genotype can contribute substantially to timely detection of this disorder.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:034858
Promotion2023
Study programmeTitle: Doctoral study
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti u području prirodnih znanosti (doktor/doktorica znanosti u području prirodnih znanosti)
Type of resourceText
File originBorn digital
Access conditionsEmbargoed access
Embargo expiration date: 2024-09-21
Terms of useLicence In copyright icon
Created on2023-11-15 12:47:00