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doctoral thesis
THE ROLE OF MITOTIC KINESINS AND CROSSLINKERS NUMA AND PRC1 IN THE MICROTUBULE POLEWARD FLUX REGULATION DURING PROMETAPHASE
Zagreb: University of Zagreb, Faculty of Science, 2023. urn:nbn:hr:217:456407
Martinčić, Jelena
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Martinčić, J. (2023). THE ROLE OF MITOTIC KINESINS AND CROSSLINKERS NUMA AND PRC1 IN THE MICROTUBULE POLEWARD FLUX REGULATION DURING PROMETAPHASE (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:456407

Martinčić, Jelena. "THE ROLE OF MITOTIC KINESINS AND CROSSLINKERS NUMA AND PRC1 IN THE MICROTUBULE POLEWARD FLUX REGULATION DURING PROMETAPHASE." Doctoral thesis, University of Zagreb, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:217:456407

Martinčić, Jelena. "THE ROLE OF MITOTIC KINESINS AND CROSSLINKERS NUMA AND PRC1 IN THE MICROTUBULE POLEWARD FLUX REGULATION DURING PROMETAPHASE." Doctoral thesis, University of Zagreb, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:217:456407

Martinčić, J. (2023). 'THE ROLE OF MITOTIC KINESINS AND CROSSLINKERS NUMA AND PRC1 IN THE MICROTUBULE POLEWARD FLUX REGULATION DURING PROMETAPHASE', Doctoral thesis, University of Zagreb, Faculty of Science, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:456407

Martinčić J. THE ROLE OF MITOTIC KINESINS AND CROSSLINKERS NUMA AND PRC1 IN THE MICROTUBULE POLEWARD FLUX REGULATION DURING PROMETAPHASE [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Science; 2023 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:217:456407

J. Martinčić, "THE ROLE OF MITOTIC KINESINS AND CROSSLINKERS NUMA AND PRC1 IN THE MICROTUBULE POLEWARD FLUX REGULATION DURING PROMETAPHASE", Doctoral thesis, University of Zagreb, Faculty of Science, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:217:456407

Metadata
TitleTHE ROLE OF MITOTIC KINESINS AND CROSSLINKERS NUMA AND PRC1 IN THE MICROTUBULE POLEWARD FLUX REGULATION DURING PROMETAPHASE
Title (croatian)ULOGA MITOTSKIH KINEZINA I VEZNIH PROTEINA NUMA I PRC1 U REGULACIJI TOKA MIKROTUBULA U PROMETAFAZI
AuthorJelena Martinčić
MentorIva Marija Tolić (mentor)
Committee memberMaja Matulić (predsjednik povjerenstva)
Committee memberMarin Barišić (član povjerenstva)
Committee memberAndreja Ambriović Ristov (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2023-12-11, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Universal decimal classification
(UDC)		577 - Biochemistry. Molecular biology. Biophysics
Abstract
Microtubule poleward flux is a fascinating phenomenon that occurs throughout mitosis, including prometaphase. Studying the involvement of different proteins in prometaphase flux regulation is challenging because of high dynamics of microtubule and chromosome movements in this phase. Here I show that the prometaphase spindle consists of k-fibers and bridging fibers, and their poleward flux velocities are similar as in metaphase. By using tubulin photoactivation and speckle microscopy experiments I defined the role of mitotic kinesins and passive crosslinkers in the flux regulation. CENP-E and HSET are generating flux at the antiparallel fibers and this force is transmitted to the k-fibers via HSET and NuMA. The congressing kinetochores are asymmetrically bioriented with more microtubules on the side of the closer pole, and interkinetochore distance close to the metaphase values. I demonstrated that kinetochores congress on long antiparallel overlaps, and the flux is generating forces that help the congression.
Abstract (english)
Tok mikrotubula prema polu je fascinantan fenomen koji se događa tijekom mitoze, uključujući i prometafazu. Proučavanje uloge različitih proteina u regulaciji toka mikrotubula je izazovno zbog velike dinamike u kretnji mikrotubula i kinetohora u toj fazi mitoze. Ovdje sam pokazala da se prometafazno vreteno sastoji od kinetohornih i premošćujućih vlakana kojima su brzine toka mikrotubula slične kao u metafazi. Koristeći metode fotoaktivacije i “speckle” mikroskopije definirala sam ulogu mitotskih kinezina i pasivnih veznika u regulaciji toka mikrotubula. CENP-E i HSET pokreću tok mikrotubula u antiparalelnim vlaknima, a ta sila se prenosi na k-vlakna pomoću proteina HSET i NuMA. Kinetohore su tijekom kongresije asimetrično biorijentirane s više mikrotubula na strani prema bližem polu, a interkinetohornom udaljenošću blizu vrijednosti u metafazi. Pokazala sam da se kongresija događa na dugim antiparalelnim snopovima, a tok mikrotubula proizvodi sile koje pomažu u kongresiji.
Keywords
Keywords (english)
Languageenglish
URN:NBNurn:nbn:hr:217:456407
Promotion2024
ProjectNumber: Grant agreement ID: 855158
Title: Molecular origins of aneuploidies in healthy and diseased human tissues (Aneuploidy)
Jurisdiction: eu
Funder: EXCELLENT SCIENCE - European Research Council (ERC)
Study programmeTitle: Doctoral study
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti u području prirodnih znanosti (doktor/doktorica znanosti u području prirodnih znanosti)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-01-11 12:06:15