prikaz prve stranice dokumenta Izolati vodenih plijesni (Oomycota) s kutikule slatkovodnih deseteronožnih rakova u Hrvatskoj
  Download
PDF 1.95 MB
master's thesis
Izolati vodenih plijesni (Oomycota) s kutikule slatkovodnih deseteronožnih rakova u Hrvatskoj
Zagreb: University of Zagreb, Faculty of Science, 2023. urn:nbn:hr:217:747244
Petrokov, Luka
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Petrokov, L. (2023). Izolati vodenih plijesni (Oomycota) s kutikule slatkovodnih deseteronožnih rakova u Hrvatskoj (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:747244

Petrokov, Luka. "Izolati vodenih plijesni (Oomycota) s kutikule slatkovodnih deseteronožnih rakova u Hrvatskoj." Master's thesis, University of Zagreb, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:217:747244

Petrokov, Luka. "Izolati vodenih plijesni (Oomycota) s kutikule slatkovodnih deseteronožnih rakova u Hrvatskoj." Master's thesis, University of Zagreb, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:217:747244

Petrokov, L. (2023). 'Izolati vodenih plijesni (Oomycota) s kutikule slatkovodnih deseteronožnih rakova u Hrvatskoj', Master's thesis, University of Zagreb, Faculty of Science, accessed 27 December 2024, https://urn.nsk.hr/urn:nbn:hr:217:747244

Petrokov L. Izolati vodenih plijesni (Oomycota) s kutikule slatkovodnih deseteronožnih rakova u Hrvatskoj [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2023 [cited 2024 December 27] Available at: https://urn.nsk.hr/urn:nbn:hr:217:747244

L. Petrokov, "Izolati vodenih plijesni (Oomycota) s kutikule slatkovodnih deseteronožnih rakova u Hrvatskoj", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:217:747244

Metadata
TitleIzolati vodenih plijesni (Oomycota) s kutikule slatkovodnih deseteronožnih rakova u Hrvatskoj
Title (english)Oomycete (Oomycota) isolates from the cuticle of crayfish species in Croatia
AuthorLuka Petrokov
MentorAna Bielen (mentor)
MentorSandra Hudina (komentor)
Committee memberSandra Hudina (predsjednik povjerenstva)
Committee memberIvana Maguire (član povjerenstva)
Committee memberMarin Ježić (član povjerenstva)
Committee memberJasna Lajtner (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2023-10-26, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Istraživanja rasprostranjenosti i utjecaja vodenih plijesni na slatkovodne deseteronožne rakove većinom su fokusirana na patogena Aphanomyces astaci, dok su u Hrvatskoj i ostalim dijelovima Europe nedovoljno istražene druge vrste vodenih plijesni koje mogu nastanjivati kutikulu rakova, iako neke od njih također mogu imati negativan utjecaj na domaćina. Cilj ovog istraživanja bio je uzgojiti i taksonomski identificirati izolate vodenih plijesni s egzoskeleta pet različitih autohtonih i invazivnih vrsta rakova u Hrvatskoj. Vodene plijesni kultivirane su iz pereiopoda i abdominalne kutikule položene na PG1 krutu hranjivu podlogu, uz precjepljivanje hifa na nove podloge do dobivanja akseničnih kultura vodenih plijesni. Identifikacija izolata do razine vrste provedena je sekvenciranjem ITS (engl. internal transcribed spacer) regije uz naknadnu filogenijsku analizu. Utvrđene su četiri vrste vodenih plijesni izolirane s kutikule dviju vrsta rakova: izolati Saprolegnia australis i Saprolegnia turfosa s kutikule vrste Pacifastacus leniusculus te izolati Aphanomyces astaci i Aphanomyces repetans s kutikule vrsta Astacus astacus i Pacifastacus leniusculus. Neke od izoliranih vodenih plijesni su saprofiti (S. turfosa, A. repetans), dok su drugi oportunisti (S. australis) ili primarni patogeni (A. astaci, uzročnik račje kuge).
Abstract (english)
Research on the distribution and effects of oomycetes on crayfish hosts is mostly focused on Aphanomyces astaci, the causative agent of the crayfish plague. In Croatia and other parts of Europe research on this topic is scarce, despite of the possibility of negative impacts on the host. The aim of this study was to cultivate and identify oomycete isolates from the exoskeleton of different native and invasive crayfish species present in Croatia. Oomycetes were cultivated from pereiopods and abdominal cuticle inoculated on PG1 solid growth media, with hyphal transfers to new media until pure cultures were obtained. Identification of the isolates to species level was performed by sequencing of the internal transcribed spacer (ITS) region followed by phylogenetic analysis. We detected four oomycete species originating from two crayfish species: Saprolegnia australis and Saprolegnia turfosa isolates originating from Pacifastacus leniusculus, Aphanomyces astaci and Aphanomyces repetans isolates originating from both Astacus astacus and Pacifastacus leniusculus. Some of the detected species are saprophytic (S. turfosa, A. repetans), while others are opportunistic (S. australis) or primary pathogens (A. astaci, the causative agent of crayfish plague).
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:747244
Study programmeTitle: Ecology and Nature Preservation-University graduate programme
Study programme type: university
Study level: graduate
Academic / professional title: sveučilišni/a magistar/magistra ekologije i zaštite prirode (sveučilišni/a magistar/magistra ekologije i zaštite prirode)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2024-10-26
Terms of useLicence In copyright icon
Created on2024-01-15 13:26:04