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master's thesis
Procjena izloženosti dagnje (Mytilus galloprovincialis Lamarck, 1819) onečišćenju mora upotrebom biomarkera oksidativnog stresa
Zagreb: University of Zagreb, Faculty of Science, 2016. urn:nbn:hr:217:124159
Sabolić, Iva
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Sabolić, I. (2016). Procjena izloženosti dagnje (Mytilus galloprovincialis Lamarck, 1819) onečišćenju mora upotrebom biomarkera oksidativnog stresa (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:124159

Sabolić, Iva. "Procjena izloženosti dagnje (Mytilus galloprovincialis Lamarck, 1819) onečišćenju mora upotrebom biomarkera oksidativnog stresa." Master's thesis, University of Zagreb, Faculty of Science, 2016. https://urn.nsk.hr/urn:nbn:hr:217:124159

Sabolić, Iva. "Procjena izloženosti dagnje (Mytilus galloprovincialis Lamarck, 1819) onečišćenju mora upotrebom biomarkera oksidativnog stresa." Master's thesis, University of Zagreb, Faculty of Science, 2016. https://urn.nsk.hr/urn:nbn:hr:217:124159

Sabolić, I. (2016). 'Procjena izloženosti dagnje (Mytilus galloprovincialis Lamarck, 1819) onečišćenju mora upotrebom biomarkera oksidativnog stresa', Master's thesis, University of Zagreb, Faculty of Science, accessed 16 February 2025, https://urn.nsk.hr/urn:nbn:hr:217:124159

Sabolić I. Procjena izloženosti dagnje (Mytilus galloprovincialis Lamarck, 1819) onečišćenju mora upotrebom biomarkera oksidativnog stresa [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2016 [cited 2025 February 16] Available at: https://urn.nsk.hr/urn:nbn:hr:217:124159

I. Sabolić, "Procjena izloženosti dagnje (Mytilus galloprovincialis Lamarck, 1819) onečišćenju mora upotrebom biomarkera oksidativnog stresa", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:217:124159

Metadata
TitleProcjena izloženosti dagnje (Mytilus galloprovincialis Lamarck, 1819) onečišćenju mora upotrebom biomarkera oksidativnog stresa
Title (english)Assessment of pollution exposure in Mytilus galloprovincialis Lamarck, 1819 using oxidative stress biomarkers
AuthorIva Sabolić
MentorAnamaria Štambuk (mentor)
Committee memberAnamaria Štambuk (predsjednik povjerenstva)
Committee memberSandra Radić Brkanac (član povjerenstva)
Committee memberAna Galov (član povjerenstva)
Committee memberMaria Špoljar (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2016-06-10, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Oksidativni stres, poremećaj u ravnoteži između produkcije slobodnih kisikovih radikala i antioksidativne obrane organizma, može dovesti do štete na staničnim strukturama i poremetiti vitalne funkcije organizma. Zaštitu od toksičnosti slobodnih radikala stanicama omogućuje kompleksni sustav antioksidativne obrane te se oksidativna oštećenja i aktivnosti antioksidativnih enzima smatraju dobrim biomarkerima za analizu utjecaja okolišnog stresa na organizam. Glavni ciljevi ovog rada bili su utvrditi utjecaj kvalitete okoliša, sezonalnosti i adaptacijskih mehanizama na biomarkere oksidativnog stresa u dagnje (Mytilus galloprovincialis). Analizirano je 6 biomarkera: katalaza, glutation reduktaza, glutation S-transferaze, acetilkolinesteraza, karbonili i malondialdehidi. Biomarkeri su spektrofotometrijski određeni u 15 nativnih populacija dagnji s istočne obale Jadranskog mora, u jedinkama izlaganim povećanim koncentracijama bakra u laboratoriju te u dagnjama transplantiranim na postaje različite kvalitete okoliša. Rezultati bioloških analiza su integrirani s kemijskim analizama tkiva dagnji. Dobiveni rezultati su pokazali iznimnu varijabilnost svih biomarkera. Utvrđena je sezonalna varijabilnost te različit odgovor antioksidativnih enzima s obzirom na kvalitetu okoliša. Biomarkeri mjereni u kavezno izlaganim jedinkama najbolji su pokazatelj kvalitete okoliša. Jedinke u mezokozmos eksperimentu nisu pokazale populacijski učinak fenotipskog odgovora na stres te nisu utvrđene nedvosmislene korelacije između koncetracija određenih teških metala u tkivu nativnih jedinki i indukcije specifičnih biomarkera.
Abstract (english)
Oxidative stress, a disturbance in the balance between the production of reactive oxygen species and antioxidant defenses, can result in damage to cell structures and disturbance of vital functions. Protection against toxicity of free radicals is provided by a complex antioxidative defense system and, consequently, oxidative damage and activity of antioxidant enzymes are considered useful biomarkers for analysis of environmental stress responses in organisms. The main purpose of this work was to identify the effect of environmental quality, seasonality and adaptation mechanisms on oxidative stress biomarkers in mussels (Mytilus galloprovincialis). 6 biomarkers were analysed: catalase, glutathione reductase, glutathione S-transferases, acetylcholinesterase, carbonyl content and malondialdehyde. The biomarkers were determined by spectrophotometry in 15 native mussels populations from east Adriatic coast, in individuals exposed to copper in mesocosm and mussels transplanted to sites of different environmental quality. Biological analyses were integrated with data from chemical analyses of mussels tissue. Results showed high variability of biomarker responses. Sesonal variability and diverse response of antioxidative enzymes to different environment quality was detected. Biomarkers measured in caged mussels provided the best indication of the envrionemntal quality. Mussels in mesocosm experiment didn't show population effect of phenotypic stress response, nor were unambiguous correlations found between specific heavy metals and biomarker induction in native populations.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:124159
Study programmeTitle: Ecology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra ekologije i zaštite prirode (magistar/magistra ekologije i zaštite prirode)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-05-12 08:24:50