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master's thesis
Vezanje indolnih spojeva na ljudski albumin
Zagreb: University of Zagreb, Faculty of Science, 2016. urn:nbn:hr:217:474216
Sekelj, Maja
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Sekelj, M. (2016). Vezanje indolnih spojeva na ljudski albumin (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:474216

Sekelj, Maja. "Vezanje indolnih spojeva na ljudski albumin." Master's thesis, University of Zagreb, Faculty of Science, 2016. https://urn.nsk.hr/urn:nbn:hr:217:474216

Sekelj, Maja. "Vezanje indolnih spojeva na ljudski albumin." Master's thesis, University of Zagreb, Faculty of Science, 2016. https://urn.nsk.hr/urn:nbn:hr:217:474216

Sekelj, M. (2016). 'Vezanje indolnih spojeva na ljudski albumin', Master's thesis, University of Zagreb, Faculty of Science, accessed 18 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:474216

Sekelj M. Vezanje indolnih spojeva na ljudski albumin [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2016 [cited 2024 November 18] Available at: https://urn.nsk.hr/urn:nbn:hr:217:474216

M. Sekelj, "Vezanje indolnih spojeva na ljudski albumin", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:217:474216

Metadata
TitleVezanje indolnih spojeva na ljudski albumin
Title (english)BINDING OF INDOLES TO HUMAN SERUM ALBUMIN
AuthorMaja Sekelj
MentorMarina Ilakovac Kveder (mentor)
MentorPetra Peharec Štefanić (mentor)
Committee memberPetra Peharec Štefanić (predsjednik povjerenstva)
Committee memberAna Galov (član povjerenstva)
Committee memberŽeljka Vidaković-Cifrek (član povjerenstva)
Committee memberMaja Matulić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2016-04-25, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Albumin (HSA) jedan je od najvažnijih transportnih proteina u ljudskom serumu. Albumin veže različite endogene i egzogene tvari. U patološkim stanjima kao što je zatajenje bubrega te u bolestima jetre, dolazi do nakupljanja uremičnih spojeva koji se vežu na albumin i na taj način inhibiraju vezanje lijekova pa terapija nije učinkovita. Jedan od uremičnih spojeva je indol-3-octena kiselina (IAA) za koju je dokazano da se specifično veže na albumin u patološkim stanjima. U ovom radu istraženo je vezanje IAA, L-triptofana, 5-metil-IAA i 5-benziloksi-IAA na HSA u prisustvu i odsustvu vezanih masnih kiselina koje se normalno u organizmu vežu na HSA. Metodom ravnotežne dijalize i fluorescencijske spektroskopije određena je koncentracija nevezanog i vezanog liganda te su podaci analizirani grafičkim prikazom prema Scatchardu. Indolni spojevi se vežu specifično na jedno vezno mjesto u albuminu s različitim afinitetima. Najveći afinitet ima IAA u odnosu na ostale proučavane indolne spojeve.
Abstract (english)
Human serum albumin (HSA) is one of the most important transport proteins in human serum. Albumin binds various endogenous and exogenous substances. In pathological conditions such as renal failure and in liver disease, there are present uremic compounds which bind to albumin and thereby inhibit the binding of drugs leading to ineffective therapies. One of the uremic compounds is indole-3-acetic acid (IAA) which has been shown to specifically bind to albumin in pathological conditions. This study investigated the binding of IAA, L- tryptophan, 5-methyl-IAA and 5-benzyloxy-IAA to HSA in the absence and presence of fatty acids, which are normally bound to HSA. By using methods of equilibrium dialysis and fluorescence spectroscopy, the concentrations of unbound and bound ligands were determined, and the data were analyzed according to Scatchard plot. Indole compounds are binding specifically to one binding site of the albumin with different affinities. IAA binds with the highest affinity as compared to other studied indoles.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:474216
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-05-12 08:28:03