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master's thesis
Raznolikost gena DRB i DQA/DQB/DRB haplotipova skupine II glavnog sustava tkivne podudarnosti u čagljeva (Canis aureus) iz istočne Europe
Zagreb: University of Zagreb, Faculty of Science, 2016. urn:nbn:hr:217:990111
Šešelja, Kate
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Šešelja, K. (2016). Raznolikost gena DRB i DQA/DQB/DRB haplotipova skupine II glavnog sustava tkivne podudarnosti u čagljeva (Canis aureus) iz istočne Europe (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:990111

Šešelja, Kate. "Raznolikost gena DRB i DQA/DQB/DRB haplotipova skupine II glavnog sustava tkivne podudarnosti u čagljeva (Canis aureus) iz istočne Europe." Master's thesis, University of Zagreb, Faculty of Science, 2016. https://urn.nsk.hr/urn:nbn:hr:217:990111

Šešelja, Kate. "Raznolikost gena DRB i DQA/DQB/DRB haplotipova skupine II glavnog sustava tkivne podudarnosti u čagljeva (Canis aureus) iz istočne Europe." Master's thesis, University of Zagreb, Faculty of Science, 2016. https://urn.nsk.hr/urn:nbn:hr:217:990111

Šešelja, K. (2016). 'Raznolikost gena DRB i DQA/DQB/DRB haplotipova skupine II glavnog sustava tkivne podudarnosti u čagljeva (Canis aureus) iz istočne Europe', Master's thesis, University of Zagreb, Faculty of Science, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:990111

Šešelja K. Raznolikost gena DRB i DQA/DQB/DRB haplotipova skupine II glavnog sustava tkivne podudarnosti u čagljeva (Canis aureus) iz istočne Europe [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2016 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:217:990111

K. Šešelja, "Raznolikost gena DRB i DQA/DQB/DRB haplotipova skupine II glavnog sustava tkivne podudarnosti u čagljeva (Canis aureus) iz istočne Europe", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:217:990111

Metadata
TitleRaznolikost gena DRB i DQA/DQB/DRB haplotipova skupine II glavnog sustava tkivne podudarnosti u čagljeva (Canis aureus) iz istočne Europe
Title (english)Variability of major histocompability complex DRB class II gene and DQA/DQB/DRB haplotyps in the golden jachals (Canis aureus) from easternEurope
AuthorKate Šešelja
MentorAna Galov (mentor)
Committee memberAna Galov (predsjednik povjerenstva)
Committee memberMirta Tkalec (član povjerenstva)
Committee memberRenata Matoničkin Kepčija (član povjerenstva)
Committee memberBožena Mitić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2016-02-29, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Glavni sustav tkivne podudarnosti (MHC) ima ključnu imunoregulacijsku ulogu kod sisavaca. Raznolikost MHC gena omogućuje prepoznavanje širokog spektra patogena, a time i povećava izglede za preživljavanje vrste. Važan su molekularni marker za proučavanje adaptivne evolucije populacija i vrsta. Čagalj (Canis aureus) pripada porodici Canidae (psi), podred Caniformia, redu Carnivora (mesojedi). Unatoč svojoj širokoj rasprostranjenosti, čagalj je slabo istraživana vrsta i samo je nekoliko genetičkih istraživanja ove vrste objavljeno do danas. Cilj ovog rada je odrediti raznolikost gena DRB lokusa i DQA1/DQB1/DRB1 haplotipova skupine II glavnog sustava tkivne podudatnosti na uzorcima čagljeva istočne Europe. Iz 21 uzorka čaglja, postupcima izolacije DNA, PCR metodom, elektroforezom, sekvenciranjem i molekularnim kloniranjem pronašla sam pet alela na DRB lokusu. Svi aleli imaju jedinstveni aminokiselinski slijed, a dva alela su novoutvrđena te sam im dala radne nazive DRB*14aEst i DRB*15aEst. Alel DRB1*00901 je poznat iz prethodnih istraživanja na ostalim vrstama porodice pasa što potvrđuje postojanje trans-specijskog polimorfizma. dN/dS testom je potvrđena pretpostavka djelovanja pozitivne selekcije na DRB lokusu. Pronašla sam ukupno sedam jedinstvenih haplotipova u istraženom uzorku, a koristila sam alele dobivene za DQA i DQB lokuse na istom uzorku u ranijem istraživanju.
Abstract (english)
The genes of major histocompatibility (MHC) system are a key component of the immune system in mammals. The diversity of MHC genes enables the recognition of a wider range of pathogens, and thus increases the chances of survival. They are important molecular markers for studying adaptive genetic diversity in populations and species. Golden jackal (Canis aureus) belongs to the family Canidae (dogs), suborder Caniformia, order Carnivora (carnivores). So far there are only a few published genetic studies concerning golden jackal although it is a widespread species. The aim of this graduate thesis is to determine the diversity of the MHC DRB class II gene in golden jackals from Eastern Europe. Using the methods of DNA isolation, PCR method, electrophoresis, sequencing and molecular cloning, I have identified five alleles at DRB locus from 21 samples. All five alleles has unique amino acid sequence, and two alleles have been newly found and named DRB*14aEst and DRB*15aEst. The allele DRB*00901 is known from previous research of other species in the dog family, which confirms the existence of a trans-species polymorphism. The dN/dS (ratio of non-synonymous versus synonymous substitutions) test has confirmed the assumption that DRB loci are influenced by positive selection. In the examined sample, total of seven unique haplotypes have been identified. For inference of haplotypes I used alleles found at DQA and DQB loci of the same samples identified in previous research.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:990111
Study programmeTitle: Experimental Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-05-12 08:51:43