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doctoral thesis
Evolucijska i prostorno-vremenska usporedba N-glikoma mozga
Zagreb: University of Zagreb, Faculty of Science, 2017. urn:nbn:hr:217:152986
Gudelj, Ivan
University of Zagreb
Faculty of Science
Department of Chemistry

Institutional repository: Repository of the Faculty of Science

Cite this document

Gudelj, I. (2017). Evolucijska i prostorno-vremenska usporedba N-glikoma mozga (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:152986

Gudelj, Ivan. "Evolucijska i prostorno-vremenska usporedba N-glikoma mozga." Doctoral thesis, University of Zagreb, Faculty of Science, 2017. https://urn.nsk.hr/urn:nbn:hr:217:152986

Gudelj, Ivan. "Evolucijska i prostorno-vremenska usporedba N-glikoma mozga." Doctoral thesis, University of Zagreb, Faculty of Science, 2017. https://urn.nsk.hr/urn:nbn:hr:217:152986

Gudelj, I. (2017). 'Evolucijska i prostorno-vremenska usporedba N-glikoma mozga', Doctoral thesis, University of Zagreb, Faculty of Science, accessed 31 December 2024, https://urn.nsk.hr/urn:nbn:hr:217:152986

Gudelj I. Evolucijska i prostorno-vremenska usporedba N-glikoma mozga [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Science; 2017 [cited 2024 December 31] Available at: https://urn.nsk.hr/urn:nbn:hr:217:152986

I. Gudelj, "Evolucijska i prostorno-vremenska usporedba N-glikoma mozga", Doctoral thesis, University of Zagreb, Faculty of Science, Zagreb, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:217:152986

Metadata
TitleEvolucijska i prostorno-vremenska usporedba N-glikoma mozga
Title (english)Evolutionary and spatio-temporal comparison of the brain N-glycomes
AuthorIvan Gudelj
MentorGordan Lauc (mentor)
Committee memberMarko Močibob (predsjednik povjerenstva)
Committee memberOlga Gornik (član povjerenstva)
Committee memberSilva Katušić Hećimović (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Chemistry)
Zagreb
Defense date and country2017-04-27, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Inorganic Chemistry
Universal decimal classification
(UDC)		54 - Chemistry. Crystallography. Mineralogy
Abstract
N-glikozilacija proteina je ključna za razvoj i funkciju živčanog sustava. U ovoj disertaciji konstruiran je prostorno-vremenski glikomski profil mozga, istražujući N-glikanske strukture četiriju funkcionalno različitih regija mozga, fronatalna dorsalna kora velikog mozga, hipokampus, strijatum te mali mozak, i njihovu raspodjelu u mozgu odraslog čovjeka; kao i u mozgu čovjeka tijekom fetalnog razvoja. Također, s namjerom da se dobije evolucijska perspektiva N-glikanskih struktura unutar različitih regija mozga, napravljena je usporedba odgovarajućih regija mozga između glikoma mozga čovjeka, čimpanzi, makaki majmuna i štakora. Određivanje glikanskih profila uzoraka mozga je provedeno korištenjem dvaju analitičkih pristupa: tekućinske kromatografije ultravisoke djelotvornosti i spektrometrije masa. Uvidom u N-glikom analiziranih uzoraka, uočena je značajna razlika između različitih regija, vrsta i razvoja mozga. Razlika između N-glikoma je bila veća što su vrste bile evolucijski udaljenije. N-glikanski profil malog mozga se najviše razlikovao od ostalih analiziranih regija. Razvijena HILIC-UPLC metoda za analizu N-glikana tkiva mozga se pokazala reproducibilnom i robusnom.
Abstract (english)
Protein N-glycosylation is crucial for the development and function of the nervous system. In this dissertation, a spatio-temporal profile of the brain glycome was constructed by investigating N-glycan structures of four functionally distinct brain regions, the dorsal fronatal cortex, the hippocampus, the striatum and the cerebellum, and their distribution within the adult human brain as well as the fetal. Moreover, in order to obtain an evolutionary perspective of the corresponding brain regions, a cross-species comparison was made between the human, the chimpanzee, the macaque and the rat. Two analytical approaches were used for glycosylation profiling of brain samples: ultra-performance liquid chromatography and mass spectrometry. Significant difference was noticed between the regions, the species and the developmental stages in the analyzed N-glycomes. The difference between species was higher as they were evolutionary more distinct. The cerebellar N-glycom was the most distinctive of all the analyzed brain regions. The developed HILIC-UPLC method for the N-glycans analysis of the brain tissue was reproducible and robust.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:152986
Study programmeTitle: Doctoral study in chemistry
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje kemija (doktor/doktorica znanosti, područje prirodnih znanosti, polje kemija)
Type of resourceText
Extent133 str. ; 30 cm
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-05-12 09:18:15