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doctoral thesis
Povezanost učestalosti polimorfizama gena FasL i FOXO3 s razinama citokina TNFα i IL-10 u reumatoidnom artritisu
Zagreb: University of Zagreb, Faculty of Science, 2016. urn:nbn:hr:217:119084
Artuković, Marinko
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Artuković, M. (2016). Povezanost učestalosti polimorfizama gena FasL i FOXO3 s razinama citokina TNFα i IL-10 u reumatoidnom artritisu (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:119084

Artuković, Marinko. "Povezanost učestalosti polimorfizama gena FasL i FOXO3 s razinama citokina TNFα i IL-10 u reumatoidnom artritisu." Doctoral thesis, University of Zagreb, Faculty of Science, 2016. https://urn.nsk.hr/urn:nbn:hr:217:119084

Artuković, Marinko. "Povezanost učestalosti polimorfizama gena FasL i FOXO3 s razinama citokina TNFα i IL-10 u reumatoidnom artritisu." Doctoral thesis, University of Zagreb, Faculty of Science, 2016. https://urn.nsk.hr/urn:nbn:hr:217:119084

Artuković, M. (2016). 'Povezanost učestalosti polimorfizama gena FasL i FOXO3 s razinama citokina TNFα i IL-10 u reumatoidnom artritisu', Doctoral thesis, University of Zagreb, Faculty of Science, accessed 29 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:119084

Artuković M. Povezanost učestalosti polimorfizama gena FasL i FOXO3 s razinama citokina TNFα i IL-10 u reumatoidnom artritisu [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Science; 2016 [cited 2024 November 29] Available at: https://urn.nsk.hr/urn:nbn:hr:217:119084

M. Artuković, "Povezanost učestalosti polimorfizama gena FasL i FOXO3 s razinama citokina TNFα i IL-10 u reumatoidnom artritisu", Doctoral thesis, University of Zagreb, Faculty of Science, Zagreb, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:217:119084

Metadata
TitlePovezanost učestalosti polimorfizama gena FasL i FOXO3 s razinama citokina TNFα i IL-10 u reumatoidnom artritisu
Title (english)Association of FasL and FOXO3 genes polymorphisms frequency with levels of TNFα and IL-10 cytokines in rheumatoid arthritis
AuthorMarinko Artuković
MentorAsija Stipić Marković (mentor)
MBZ: 70156
MentorTomislav Kelava (sumentor)
MBZ: 291360
Committee memberPetra Korać (predsjednik povjerenstva)
Committee memberDanka Grčević (član povjerenstva)
MBZ: 210400
Committee memberAna Galov (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2016, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Universal decimal classification
(UDC)		57 - Biological sciences. Physical anthropology. Biodiversity
Abstract
Polimorfizmi jednog nukleotida (SNP, engl. single nucleotide polymorphism) gena FOXO3 i FasL mogu biti povezani s razvojem i tijekom reumatoidnog artritisa (RA). SNP gena FasL povećava rizik oboljevanja od RA. Oba su gena uključena u apoptozu, pri čemu transkripcijski faktor FOXO3 supresijom Fas liganda sprječava apoptozu i podržava tijek kronične upale. Izolacijom DNA iz periferne krvi, određivanjem prisutnosti SNP-a u navedenim genima, određivanjem razine interleukina-10 (IL-10) i čimbenika tumorske nekroze α (TNFα, prema engl. tumor necrosis factor) iz krvi imunoenzimskom metodom ELISA, te korelacijom prisustva SNP-ova s promjenama razine citokina analizirani su učestalost polimorfizama, ekspresija proupalnih (TNFα) i protuupalnih (IL-10) citokina u bolesnika s reumatoidnim artritisom u odnosu na kontrolnu skupinu. Polimorfizmi gena FOXO3 i FasL u ispitivanom uzorku hrvatske populacije nisu promijenjene učestalosti u RA, te nisu jasno povezani su s promjenom ekspresije citokina IL-10 i TNFα.
Abstract (english)
FOXO3 and FasL single nucleotide polymorphisms (SNP) could be related to the course of rheumatoid arthritis (RA) due to changed expression of certain cytokines. FasL gene SNP increases the susceptibility to RA. Both genes are involved in apoptosis, with FOXO3 transcription factor suppression of Fas ligand preventing apoptosis and sustaining chronic inflammation. We determined the frequency of SNPs, expression of proinflammatory (TNFα) and anti-inflammatory (IL-10) cytokines in RA patients by methods of DNA isolation from peripheral blood, genotyping, determination of TNFα and IL-10 (ELISA) and than correlated polymorphisms with expression of cytokines. Results: In our cohort FOXO3 and FasL polymorphisms does not differ in frequency in RA, and are not related to expression changes of TNFα and IL-10 with consequences to the course of RA.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:119084
Study programmeTitle: Biology
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-05-12 09:18:15