A multispecies biofilm is a microbial community consisting of various microbial species. Microorganisms at the water-air interface form biofilms. The research focuses on observing the formation of multispecies biofilm involving yeast Saccharomyces cerevisiae and bacteria Acinetobacter baumannii, Acinetobacter junii, Staphylococcus aureus, Bacillus cereus. All organisms in the study contributed to the biofilm formation. Biofilms created by certain organisms were more present and formed in a shorter time frame. Microorganisms within the formed biofilm moved along the glass, non-nutritive surface. The bacteria Acinetobacter junii, Acinetobacter baumannii, Bacillus cereus and Staphylococcus aureus moved along the glass independently or together with bacteria of another species. Yeast Saccharomyces cerevisiae along the glass moves with other bacteria. The research investigated the dependence of organisms on mobility and the interaction between prokaryotes and eukaryotes within the multispecies biofilm. Using a fluorescence microscope, the study examined the abundance of polyphosphates in the cell and their impact on bacterial mobility in phosphate-accumulating bacteria.