prikaz prve stranice dokumenta Molekularne značajke marokanskog virusa mozaika lubenice prvi put zabilježenog u Hrvatskoj
Embargo period 2024-12-05
master's thesis
Molekularne značajke marokanskog virusa mozaika lubenice prvi put zabilježenog u Hrvatskoj
Zagreb: University of Zagreb, Faculty of Science, 2024. urn:nbn:hr:217:805264
Marohnić, Marko
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Marohnić, M. (2024). Molekularne značajke marokanskog virusa mozaika lubenice prvi put zabilježenog u Hrvatskoj (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:805264

Marohnić, Marko. "Molekularne značajke marokanskog virusa mozaika lubenice prvi put zabilježenog u Hrvatskoj." Master's thesis, University of Zagreb, Faculty of Science, 2024. https://urn.nsk.hr/urn:nbn:hr:217:805264

Marohnić, Marko. "Molekularne značajke marokanskog virusa mozaika lubenice prvi put zabilježenog u Hrvatskoj." Master's thesis, University of Zagreb, Faculty of Science, 2024. https://urn.nsk.hr/urn:nbn:hr:217:805264

Marohnić, M. (2024). 'Molekularne značajke marokanskog virusa mozaika lubenice prvi put zabilježenog u Hrvatskoj', Master's thesis, University of Zagreb, Faculty of Science, accessed 03 May 2024, https://urn.nsk.hr/urn:nbn:hr:217:805264

Marohnić M. Molekularne značajke marokanskog virusa mozaika lubenice prvi put zabilježenog u Hrvatskoj [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2024 [cited 2024 May 03] Available at: https://urn.nsk.hr/urn:nbn:hr:217:805264

M. Marohnić, "Molekularne značajke marokanskog virusa mozaika lubenice prvi put zabilježenog u Hrvatskoj", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:217:805264

Metadata
TitleMolekularne značajke marokanskog virusa mozaika lubenice prvi put zabilježenog u Hrvatskoj
Title (english)Molecular characteristics of Moroccan watermelon mosaic virus detected in Croatia for the first time
AuthorMarko Marohnić
MentorDijana Škorić (mentor)
Committee memberDijana Škorić (predsjednik povjerenstva)
Committee memberRenata Šoštarić (član povjerenstva)
Committee memberMirta Tkalec (član povjerenstva)
Committee memberMaja Matulić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2024-02-05, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Marokanski virus mozaika lubenice (eng. Morrocan watermelon mosaic virus, MWMV) pripadnik je roda Potyvirus unutar porodice Potyviridae. Ekonomski je značajan virus koji na zaraženim biljkama uzrokuje teške simptome i time dovodi do velikih gubitaka pri uzgoju tikvenjača (biljke roda Cucurbita). Virus je prvi put identificiran 1972. godine u Maroku, a do sada njegova prisutnost nije bila zabilježena u Hrvatskoj. Cilj ovog diplomskog rada bio je potvrditi infekciju MWMV-om na uzorcima simptomatičnih tikvenjača prikupljenih na području Pitomače 2021. i 2022. i time potvrditi prisutnost MWMV-a na teritoriju Republike Hrvatske. Također, cilj je bio i istražiti molekularne značajke izolata novozabilježenog virusa i utvrditi kojoj filogeografskoj skupini bi hrvatski izolati mogli pripadati. Metodom reverzne transkripcije i lančane reakcije polimerazom (RT-PCR) potvrđena je infekcija MWMV-om kod šest uzoraka tikvica, a filogenetskim analizama amplikona utvrđena je pripadnost hrvatskih izolata istoj filogeografskoj skupini u kojoj se nalaze izvorni izolati iz Maroka.
Abstract (english)
The Moroccan watermelon mosaic virus (MWMV), a member of the Potyvirus genus within the Potyviridae family, is an economically significant virus that causes severe symptoms on infected plants leading to substantial losses in the cultivation of cucurbits (plants of the Cucurbita genus). The virus was first identified in Morocco in 1972 and has not been previously reported in Croatia. The aim of this thesis was to confirm MWMV infection in symptomatic cucurbit samples collected in the Pitoma č a region in 2021 and 2022, thereby confirming the presence of MWMV in the territory of the Republic of Croatia. Additionally, the goal was to molecularly characterize the newly detected virus isolates and determine its phylogeographic group ing . MWMV infection was confirmed in six zucchini samples using the reverse transcription polymerase chain reaction (RT PCR) method . P hylogenetic analysis of the amplicons revealed that the Croatian isolates belong to the same phylogeographic group as the original isolates from Morocco.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:805264
ProjectNumber: IPS-2020-01-2960
Title: Visokoprotočno Nanopore-sekvenciranje mikrobnih genoma u rješavanju problema dijagnostike i epidemiologije biljnih patogena
Title: High-throughput Nanopore-sequencing of microbial genomes in solving problems of diagnosis and epidemiology of plant pathogens
Leader: Dijana Škorić
Jurisdiction: Croatia
Funder: HRZZ
Funding stream: IPS
Study programmeTitle: Molecular Biology-University graduate programme
Study programme type: university
Study level: graduate
Academic / professional title: sveučilišni/a magistar/magistra molekularne biologije (sveučilišni/a magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsEmbargoed access
Embargo expiration date: 2024-12-05
Terms of useLicence In copyright icon
Created on2024-02-27 11:31:53