prikaz prve stranice dokumenta Position of chromosomes 1 and 9, labeled using CRISPR/Cas9 technology, with respect to the nuclear envelope and centrosomes
Embargo period 2026-02-20
master's thesis
Position of chromosomes 1 and 9, labeled using CRISPR/Cas9 technology, with respect to the nuclear envelope and centrosomes
Zagreb: University of Zagreb, Faculty of Science, 2024. urn:nbn:hr:217:632137
Mikec, Petra
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Mikec, P. (2024). Position of chromosomes 1 and 9, labeled using CRISPR/Cas9 technology, with respect to the nuclear envelope and centrosomes (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:632137

Mikec, Petra. "Position of chromosomes 1 and 9, labeled using CRISPR/Cas9 technology, with respect to the nuclear envelope and centrosomes." Master's thesis, University of Zagreb, Faculty of Science, 2024. https://urn.nsk.hr/urn:nbn:hr:217:632137

Mikec, Petra. "Position of chromosomes 1 and 9, labeled using CRISPR/Cas9 technology, with respect to the nuclear envelope and centrosomes." Master's thesis, University of Zagreb, Faculty of Science, 2024. https://urn.nsk.hr/urn:nbn:hr:217:632137

Mikec, P. (2024). 'Position of chromosomes 1 and 9, labeled using CRISPR/Cas9 technology, with respect to the nuclear envelope and centrosomes', Master's thesis, University of Zagreb, Faculty of Science, accessed 24 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:632137

Mikec P. Position of chromosomes 1 and 9, labeled using CRISPR/Cas9 technology, with respect to the nuclear envelope and centrosomes [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2024 [cited 2024 November 24] Available at: https://urn.nsk.hr/urn:nbn:hr:217:632137

P. Mikec, "Position of chromosomes 1 and 9, labeled using CRISPR/Cas9 technology, with respect to the nuclear envelope and centrosomes", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:217:632137

Metadata
TitlePosition of chromosomes 1 and 9, labeled using CRISPR/Cas9 technology, with respect to the nuclear envelope and centrosomes
Title (croatian)Položaj kromosoma 1 i 9, obilježenih pomoću tehnologije CRISPR/Cas9, u odnosu na jezgrinu ovojnicu i centrosome
AuthorPetra Mikec
MentorIva Marija Tolić (mentor)
Committee memberIva Marija Tolić (predsjednik povjerenstva)
Committee memberMaja Matulić (član povjerenstva)
Committee memberPetra Peharec Štefanić (član povjerenstva)
Committee memberBiljana Balen (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2024-02-20, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Human chromosomes occupy specific territories in the cell nucleus. Chromosomes that are localized at the periphery of the cell nucleus at the onset of mitosis show increased missegregation rates, in compromised mitosis conditions, than the chromosomes that are localized centrally. I specifically labeled subtelomeric region of chromosome 1 and pericentromeric region of chromosome 9 using technology CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats and CRISPR-associated protein 9). I determined their position in the cell nucleus, during early prometaphase, with respect to the nuclear envelope and centrosomes. Chromosome 1 is localized at the periphery, whereas chromosome 9 is equally distributed in the cell nucleus during early prometaphase. Furthermore, chromosome 1 and chromosome 9 are both equally polar (closing the angle of >90º with the spindle pole) during early prometaphase. Also, there is no difference in the distance between chromosome 1 and chromosome 9 from the centrosome in early prometaphase and the average distance of the centrosome to the former nuclear envelope in early prometaphase is 2.79 μm. The position of centrosomes during early prometaphase is important from the point of mitotic fidelity. The results of this thesis further support future research of specific chromosome position during early prometaphase.
Abstract (croatian)
Kromosomi ljudskih stanica zauzimaju specifične teritorije u jezgri. Kromosomi koji se nalaze na periferiji jezgre prije početka mitoze skloniji su pogrešnoj podjeli, u uvjetima narušene mitoze, u odnosu na kromosome koji su pozicionirani centralno. Specifično sam obilježila subtelomernu regiju kromosoma 1 i pericentromernu regiju kromosoma 9 upotrebom tehnologije CRISPR/Cas9 (engl. clustered regularly interspaced short palindromic repeats and CRISPR-associated protein 9). Odredila sam položaj navedenih kromosoma u jezgri, tijekom rane prometafaze, u odnosu na jezgrinu ovojnicu i centrosome. Kromosom 1 zauzima periferni položaj, dok je kromosom 9 podjednako distribuiran u svim dijelovima jezgre tijekom rane prometafaze. Nadalje, kromosom 1 i kromosom 9 su podjednako polarni (zatvaraju kut >90º s polom diobenog vretena) tijekom rane prometafaze. Također, ne postoji razlika u udaljenosti kromosoma 1 i kromosoma 9 od centrosoma u ranoj prometafazi te prosječna udaljenost centrosoma od nekadašnje jezgrine ovojnice tijekom rane prometafaze iznosi 2,79 μm. Pozicija centrosoma tijekom rane prometafaze važna je za pravilnu diobu. Rezultati proizašli iz ovoga rada podupiru buduća istraživanja položaja specifičnih kromosoma tijekom rane prometafaze.
Keywords
Keywords (croatian)
Languageenglish
URN:NBNurn:nbn:hr:217:632137
ProjectNumber: Grant agreement ID: 855158
Title: Molecular origins of aneuploidies in healthy and diseased human tissues (Aneuploidy)
Jurisdiction: eu
Funder: EXCELLENT SCIENCE - European Research Council (ERC)
Study programmeTitle: Molecular Biology-University graduate programme
Study programme type: university
Study level: graduate
Academic / professional title: sveučilišni/a magistar/magistra molekularne biologije (sveučilišni/a magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsEmbargoed access
Embargo expiration date: 2026-02-20
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Created on2024-02-28 08:07:46