prikaz prve stranice dokumenta Utjecaj polimorfizma u genima za biosintezu gangliozida i gangliozidnoga sastava membrana limfocita T na razvoj karcinoma vrata maternice
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doctoral thesis
Utjecaj polimorfizma u genima za biosintezu gangliozida i gangliozidnoga sastava membrana limfocita T na razvoj karcinoma vrata maternice
Zagreb: University of Zagreb, Faculty of Science, 2016. urn:nbn:hr:217:265561
Danolić, Damir
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Danolić, D. (2016). Utjecaj polimorfizma u genima za biosintezu gangliozida i gangliozidnoga sastava membrana limfocita T na razvoj karcinoma vrata maternice (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:265561

Danolić, Damir. "Utjecaj polimorfizma u genima za biosintezu gangliozida i gangliozidnoga sastava membrana limfocita T na razvoj karcinoma vrata maternice." Doctoral thesis, University of Zagreb, Faculty of Science, 2016. https://urn.nsk.hr/urn:nbn:hr:217:265561

Danolić, Damir. "Utjecaj polimorfizma u genima za biosintezu gangliozida i gangliozidnoga sastava membrana limfocita T na razvoj karcinoma vrata maternice." Doctoral thesis, University of Zagreb, Faculty of Science, 2016. https://urn.nsk.hr/urn:nbn:hr:217:265561

Danolić, D. (2016). 'Utjecaj polimorfizma u genima za biosintezu gangliozida i gangliozidnoga sastava membrana limfocita T na razvoj karcinoma vrata maternice', Doctoral thesis, University of Zagreb, Faculty of Science, accessed 24 December 2024, https://urn.nsk.hr/urn:nbn:hr:217:265561

Danolić D. Utjecaj polimorfizma u genima za biosintezu gangliozida i gangliozidnoga sastava membrana limfocita T na razvoj karcinoma vrata maternice [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Science; 2016 [cited 2024 December 24] Available at: https://urn.nsk.hr/urn:nbn:hr:217:265561

D. Danolić, "Utjecaj polimorfizma u genima za biosintezu gangliozida i gangliozidnoga sastava membrana limfocita T na razvoj karcinoma vrata maternice", Doctoral thesis, University of Zagreb, Faculty of Science, Zagreb, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:217:265561

Metadata
TitleUtjecaj polimorfizma u genima za biosintezu gangliozida i gangliozidnoga sastava membrana limfocita T na razvoj karcinoma vrata maternice
Title (english)Role of ganglioside biosynthesis genetic polymorphism and gangliosides expression in T lymphocytes membranes in cervical cancer development
AuthorDamir Danolić
MentorMarija Heffer (mentor)
MentorMario Puljiz (sumentor)
MBZ: 337403
Committee memberMaja Matulić (predsjednik povjerenstva)
Committee memberSvjetlana Kalanj-Bognar (član povjerenstva)
MBZ: 202561
Committee memberSlavko Orešković (član povjerenstva)
MBZ: 205340
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2016, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Universal decimal classification
(UDC)		576 - Cellular and subcellular biology. Cytology
Abstract
Katedra za medicinsku biologiju i genetiku, Medicinski fakultet u Osijeku Karcinom vrata maternice najčešći je karcinom ženskog spolnog sustava u svijetu. Tijekom imunološkog odgovora na infekciju humanim papiloma virusom dolazi do premještanja T-staničnog receptora unutar lipidnih splavi limfocita T, u kojima gangliozidi imaju aktivnu ulogu u oblikovanju, stabilizaciji i biološkoj funkciji. Svrha istraživanja je utvrditi postoji li povezanost pojedinih polimorfizama u genima za biosintezu gangliozida i gangliozidnoga sastava membrana limfocita T s pojavom karcinoma vrata maternice. Genotipizacija polimorfizama gena za biosintezu gangliozida učinjena je primjenom metodologije Taqman. Sve ispitanice bile su homozigoti i imale su divlji tip alela kod svih ispitivanih gena (CC za gen B4GALT5, AA za gen ST3GAL5, AA za gen ST8SIA1 i CC za gen B4GALNT1). Analiza gangliozidnoga sastava staničnih membrana limfocita T provedena je korištenjem dvostrukog označavanja protutijelima na gangliozid GM1 i na antigene pojedinih loza limfocita. Nismo našli razliku u gangliozidnom sastavu membrana limfocita T između istraživanih skupina.
Abstract (english)
Department of Medical Biology and Genetics, Faculty of Medicine, University of Osijek Cervical cancer is the most common gynaecological cancer in women. Cell mediated immunity plays a significiant role in progression or regression of neoplastic cervical lesions caused by human papilloma virus infection. Engagement of antigen-specific T cell receptors is a prerequisite for T cell activation. The initial events of T cell activation involves movement of the T cell receptor into specialized microdomains known as lipid rafts. Gangliosides play an active role in the formation, stabilization and biological functions of lipid rafts. This study aims to determine whether polymorphisms in the genes involved in the biosynthesis of gangliosides and gangliosides expression in T cell membrane represent risk factor for cervical cancer. Taqman methods for single nucleotide polymorphism genotyping was used. All subjects carried the homozygous wild-type genotypes for all analyzed genes (CC for gene B4GALT5, AA for gene ST3GAL5, AA for gene ST8SIA1 and CC for gene B4GALNT1). Gangliosides expression in T lymphocytes membranes was analyzed using double labelling antibodies on ganglioside GM1. We have not found difference in gangliosides expression in T lymphocytes membranes between the two groups.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:265561
Study programmeTitle: Biology
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
Type of resourceText
File originBorn digital
Access conditionsClosed access
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Created on2017-05-12 09:46:38