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doctoral thesis
GENOMIKA I OSOBITOSTI PONAVLJAJUĆIH DNA ŠKOLJKAŠA IZ PORODICE OSTREIDAE
Zagreb: University of Zagreb, Faculty of Science, 2024. urn:nbn:hr:217:270005
Tunjić Cvitanić, Monika
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

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Tunjić Cvitanić, M. (2024). GENOMIKA I OSOBITOSTI PONAVLJAJUĆIH DNA ŠKOLJKAŠA IZ PORODICE OSTREIDAE (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:270005

Tunjić Cvitanić, Monika. "GENOMIKA I OSOBITOSTI PONAVLJAJUĆIH DNA ŠKOLJKAŠA IZ PORODICE OSTREIDAE." Doctoral thesis, University of Zagreb, Faculty of Science, 2024. https://urn.nsk.hr/urn:nbn:hr:217:270005

Tunjić Cvitanić, Monika. "GENOMIKA I OSOBITOSTI PONAVLJAJUĆIH DNA ŠKOLJKAŠA IZ PORODICE OSTREIDAE." Doctoral thesis, University of Zagreb, Faculty of Science, 2024. https://urn.nsk.hr/urn:nbn:hr:217:270005

Tunjić Cvitanić, M. (2024). 'GENOMIKA I OSOBITOSTI PONAVLJAJUĆIH DNA ŠKOLJKAŠA IZ PORODICE OSTREIDAE', Doctoral thesis, University of Zagreb, Faculty of Science, accessed 13 May 2024, https://urn.nsk.hr/urn:nbn:hr:217:270005

Tunjić Cvitanić M. GENOMIKA I OSOBITOSTI PONAVLJAJUĆIH DNA ŠKOLJKAŠA IZ PORODICE OSTREIDAE [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Science; 2024 [cited 2024 May 13] Available at: https://urn.nsk.hr/urn:nbn:hr:217:270005

M. Tunjić Cvitanić, "GENOMIKA I OSOBITOSTI PONAVLJAJUĆIH DNA ŠKOLJKAŠA IZ PORODICE OSTREIDAE", Doctoral thesis, University of Zagreb, Faculty of Science, Zagreb, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:217:270005

Metadata
TitleGENOMIKA I OSOBITOSTI PONAVLJAJUĆIH DNA ŠKOLJKAŠA IZ PORODICE OSTREIDAE
Title (english)GENOMICS AND PECULIARITIES OF THE REPETITIVE DNAs OF THE OYSTERS IN THE FAMILY OF OSTREIDAE
AuthorMonika Tunjić Cvitanić
MentorMiroslav Plohl (mentor)
MentorEva Šatović Vukšić (komentor)
Committee memberVišnja Besendorfer (predsjednik povjerenstva)
Committee memberĐurđica Ugarković (član povjerenstva)
Committee memberPetra Korać (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2024, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Universal decimal classification
(UDC)		577 - Biochemistry. Molecular biology. Biophysics
Abstract
Ponavljajuća DNA u mnogim organizmima čini velik, ali neistražen dio genoma. Za vrstu Crassostrea gigas bioinformatički je utvrđeno da repeatom dominantno grade satDNA i PGE, a najbrojnija satDNA je Cg170. Bioinformatički su utvrđeni satelitomi srodnih vrsta kamenica C. gigas, Crassostrea angulata, Crassostrea virginica i Ostrea edulis. U sklopu centromere C. gigas najbrojnije DNA su transpozoni i satDNA, a u heterokromatinu najbrojniji su DNA transpozoni. Više od 75 % satelitoma te vrste pokazalo je sličnost s elementima Helitron. Analizom postranih regija monomera ili nizova satDNA utvrđeno je da se samo jedna satDNA nalazi isključivo u sklopu elementa Helitron, dvjema je većina monomera/nizova monomera samostalna, a osam satDNA u podjednakim je omjerima prisutno samostalno i u sklopu elementa. Niz satDNA zajednički je svim ispitanim vrstama, a C. gigas i C. angulata dijele najveći broj zajedničkih satDNA. U C. gigas nije otkriveno tipično nakupljanje satDNA na određenim kromosomskim pozicijama, čime je u ovoj vrsti ustanovljen novi (visokoraspršeni) obrazac organizacije satDNA na razini genoma.
Abstract (english)
Repetitive DNA constitutes big, but still uninvestigated genome part. For the oyster Crassostrea gigas it was bioinformatically determined that the repeatome is mainly constituted of satDNAs and mobile elements, and the most abundant satDNA is Cg170. The satellitomes of four closely related species, C. gigas, Crassostrea angulata, Crassostrea virginica and Ostrea edulis, were bioinformatically determined. The most abundant sequences in the centromere of C. gigas are DNA transposons and satDNAs, while in heterochromatin the most abundant are DNA transposons. More than 75% of satellitome of this species showed similarity to Helitrons. Analysis of conserved boxes adjacent to monomers or tandem repeats of satDNAs showed that only one satDNA exists integrated into Helitrons, two have majority of their monomers/tandem repeats as standalone organizational form, and eight satDNAs have equally present monomers/tandem repeats in both forms. All analyzed species have several satDNAs in common, but C. gigas and C. angulata share the highest number of common satDNAs. SatDNAs in C. gigas did not show typical clustering at certain chromosomal positions but new organizational pattern: highly scattered across the genome.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:270005
Promotion2024
ProjectNumber: IP-2014-09-3183
Title: Centromerna genomika beskralježnjaka
Title: Invertebrate centromere genomics
Acronym: CENGEN
Leader: Miroslav Plohl
Jurisdiction: Croatia
Funder: HRZZ
Funding stream: IP
ProjectNumber: IP-2019-04-5522
Title: Rasvjetljavanje evolucije satelitnih DNA visokoprotočnim analizama satelitoma srodnih vrsta
Title: Satellite DNA evolution illuminated by high-throughput satellitome analyses of related species
Acronym: EvoSat
Leader: Brankica Mravinac
Jurisdiction: Croatia
Funder: HRZZ
Funding stream: IP
Study programmeTitle: Doctoral study
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti u području prirodnih znanosti (doktor/doktorica znanosti u području prirodnih znanosti)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-04-02 09:53:00