prikaz prve stranice dokumenta Molekularna karakterizacija izolata fitoplazmi ribosomske skupine 16SrV s područja sjeverozapadne Hrvatske
Embargo period 2026-04-05
master's thesis
Molekularna karakterizacija izolata fitoplazmi ribosomske skupine 16SrV s područja sjeverozapadne Hrvatske
Zagreb: University of Zagreb, Faculty of Science, 2024. urn:nbn:hr:217:892393
Lemaić, Zvonimira
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Lemaić, Z. (2024). Molekularna karakterizacija izolata fitoplazmi ribosomske skupine 16SrV s područja sjeverozapadne Hrvatske (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:892393

Lemaić, Zvonimira. "Molekularna karakterizacija izolata fitoplazmi ribosomske skupine 16SrV s područja sjeverozapadne Hrvatske." Master's thesis, University of Zagreb, Faculty of Science, 2024. https://urn.nsk.hr/urn:nbn:hr:217:892393

Lemaić, Zvonimira. "Molekularna karakterizacija izolata fitoplazmi ribosomske skupine 16SrV s područja sjeverozapadne Hrvatske." Master's thesis, University of Zagreb, Faculty of Science, 2024. https://urn.nsk.hr/urn:nbn:hr:217:892393

Lemaić, Z. (2024). 'Molekularna karakterizacija izolata fitoplazmi ribosomske skupine 16SrV s područja sjeverozapadne Hrvatske', Master's thesis, University of Zagreb, Faculty of Science, accessed 21 May 2024, https://urn.nsk.hr/urn:nbn:hr:217:892393

Lemaić Z. Molekularna karakterizacija izolata fitoplazmi ribosomske skupine 16SrV s područja sjeverozapadne Hrvatske [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2024 [cited 2024 May 21] Available at: https://urn.nsk.hr/urn:nbn:hr:217:892393

Z. Lemaić, "Molekularna karakterizacija izolata fitoplazmi ribosomske skupine 16SrV s područja sjeverozapadne Hrvatske", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:217:892393

Metadata
TitleMolekularna karakterizacija izolata fitoplazmi ribosomske skupine 16SrV s područja sjeverozapadne Hrvatske
Title (english)Molecular characterization of ribosomal group 16SrV phytoplasma isolates from north-western Croatia
AuthorZvonimira Lemaić
MentorMartina Šeruga Musić (mentor)
Committee memberMartina Šeruga Musić (predsjednik povjerenstva)
Committee memberMirela Sertić Perić (član povjerenstva)
Committee memberDraginja Mrvoš-Sermek (član povjerenstva)
Committee memberMirta Rubčić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2024-04-05, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Fitoplazme su biljne patogene bakterije bez stanične stijenke koje pripadaju rodu 'Candidatus Phytoplasma'. Nastanjuju floem biljaka i prenose se kukcima-vektorima koji su ujedno i njihovi domaćini. Fitoplazme predstavljaju veliku opasnost u poljoprivredi uništavanjem usjeva raznih poljoprivrednih kultura, a u Europi su poznate po uništavanju usjeva vinovih loza uzrokujući bolesti žutica vinove loze (eng. grapevine yellows; GY). S tom bolesti povezane su fitoplazme flavescence dorée (FDp; ribosomska skupina 16SrV) te bois noir (ribosomska podskupina 16SrXII-A; 'Ca. P. solani'). Cilj ovog diplomskog rada je bila molekularna karakterizacija izolata FDp dobivenih iz uzoraka vinove loze, crne johe i kupine prikupljenima na sjeverozapadnom području Hrvatske. Nakon potvrde prisustva fitoplazmi u izolatima triplex real‐time PCR‐om, napravljena je filogenetska analiza gena map i vmpA umnoženih PCR-om. Utvrđena je velika genetska varijabilnost oba gena, a otkriveno je potencijalnih šest novih genotipova FDp te je po prvi put napravljena molekularna karakterizacija fitoplazmatskih izolata iz kupine i crne johe u Hrvatskoj. Također, velik broj pozitivnih izolata iz crne johe pokazuje značajan potencijal širenja zaraze ovom fitoplazmom. Dobiveni rezultati omogućuju bolji uvid u epidemiologiju FDp na području sjeverozapadne Hrvatske.
Abstract (english)
Phytoplasmas are plant-pathogenic bacteria lacking cell wall that belong to the genus ‘Candidatus Phytoplasma’. They inhabit the phloem of plants and are transmitted by insect vectors that are also their natural hosts. They pose a significant threat to agriculture by destroying crops from various cultures and are known in Europe for affecting grapevine crops by causing grapevine yellows (GY) disease. This disease is associated with flavescence dorée phytoplasmas (FDp; 16SrV ribosomal group) and bois noir phytoplasmas (16SrXII-A ribosomal subgroup; ‘Ca. P solani’). This study was focused on the molecular characterization of FDp isolates obtained from samples of grapevines, black alders and blackberries collected in north-western Croatia. After confirming the presence of phytoplasmas in isolates by using triplex real‐time PCR, a phylogenetic analysis of the map and vmpA genes amplified by PCR was performed. Significant genetic variability of both genes was determined, and six potential new FDp genotiypes were discovered, along with the first molecular characterization of phytoplasma isolates from blackberries and black alders in Croatia. Moreover, numerous positive isolates from black alders indicate a significant potential for the infection spread of this phytoplasma. The obtained results provide better insight into the epidemiology of FDp in the north‐western part of Croatia.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:892393
Study programmeTitle: Biology and Chemistry Education-University integrated undergraduate and graduate programme; specializations in: Biology
Course: Biology
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: sveučilišni/a magistar/magistra edukacije biologije i kemije (sveučilišni/a magistar/magistra edukacije biologije i kemije)
Type of resourceText
File originBorn digital
Access conditionsEmbargoed access
Embargo expiration date: 2026-04-05
Terms of useLicence In copyright icon
Created on2024-04-16 11:19:14