prikaz prve stranice dokumenta Rekombinantni GABA-A receptori - model in vitro za istraživanje neurosteroida dehidroepiandrosteron sulfata
  Download
PDF 1.94 MB
master's thesis
Rekombinantni GABA-A receptori - model in vitro za istraživanje neurosteroida dehidroepiandrosteron sulfata
Zagreb: University of Zagreb, Faculty of Science, 2016. urn:nbn:hr:217:197733
Tudor, Lucija
University of Zagreb
Faculty of Science
Department of Biology

Institutional repository: Repository of the Faculty of Science

Cite this document

Tudor, L. (2016). Rekombinantni GABA-A receptori - model in vitro za istraživanje neurosteroida dehidroepiandrosteron sulfata (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:197733

Tudor, Lucija. "Rekombinantni GABA-A receptori - model in vitro za istraživanje neurosteroida dehidroepiandrosteron sulfata." Master's thesis, University of Zagreb, Faculty of Science, 2016. https://urn.nsk.hr/urn:nbn:hr:217:197733

Tudor, Lucija. "Rekombinantni GABA-A receptori - model in vitro za istraživanje neurosteroida dehidroepiandrosteron sulfata." Master's thesis, University of Zagreb, Faculty of Science, 2016. https://urn.nsk.hr/urn:nbn:hr:217:197733

Tudor, L. (2016). 'Rekombinantni GABA-A receptori - model in vitro za istraživanje neurosteroida dehidroepiandrosteron sulfata', Master's thesis, University of Zagreb, Faculty of Science, accessed 24 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:197733

Tudor L. Rekombinantni GABA-A receptori - model in vitro za istraživanje neurosteroida dehidroepiandrosteron sulfata [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2016 [cited 2024 November 24] Available at: https://urn.nsk.hr/urn:nbn:hr:217:197733

L. Tudor, "Rekombinantni GABA-A receptori - model in vitro za istraživanje neurosteroida dehidroepiandrosteron sulfata", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:217:197733

Metadata
TitleRekombinantni GABA-A receptori - model in vitro za istraživanje neurosteroida dehidroepiandrosteron sulfata
Title (english)Recombinant GABA-A receptors - in vitro model to study the effects of neurosteroid dehydroepiandrosterone sulphate
AuthorLucija Tudor
MentorDubravka Švob Štrac (mentor)
MentorVesna Benković (mentor)
Committee memberVesna Benković (predsjednik povjerenstva)
Committee memberŽeljka Vidaković-Cifrek (član povjerenstva)
Committee memberMaja Matulić (član povjerenstva)
Committee memberInga Marijanović (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2016-03-15, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Dehidroepiandrosteron-sulfat (DHEAS) je neurosteroid uključen u važne funkcije mozga poput neuralne plastičnosti, učenja, pamćenja i ponašanja, te pokazuje potencijal u liječenju različitih neuropsihijatrijskih poremećaja, uključujući i ishemijski moždani udar. Učinci DHEAS-a u središnjem živčanom sustavu posredovani su putem GABA i glutamatnog neurotransmitorskog sustava, ali i drugih. Cilj rada bio je ispitati učinke primjene DHEAS-a na rekombinantne GABAA receptore, usporedbom djelovanja ovog neurosteorida na embrionalne stanice bubrega čovjeka (HEK 293), netransficirane i stabilno transficirane GABAA receptorima 122S, te istražiti njegovo potencijalno neuroprotektivno djelovanje u modelu deprivacije kisika i glukoze, te reperfuzije in vitro (OGDR). Istraživanje je pokazalo da se DHEAS ponaša kao alosterički antagonist rekombinantnih GABAA receptora, ali da njegova produljena primjena ne utječe na broj, afinitet, kao ni na funkcionalnu povezanost receptorskih veznih mjesta. Rezultati su također pokazali da DHEAS, primijenjen i prije i nakon OGDR djeluje protektivno na stanice HEK 293, te da se citoprotektivan učinak ovog neurosteroida barem dijelom ostvaruje putem GABAA receptora.
Abstract (english)
Dehydroepiandrosterone sulfate (DHEAS) is a neurosteroid involved in various important brain functions such as neural plasticity, learning, memory and behavior, demonstrating the potential to treat a variety of neuropsychiatric disorders, including ischemic stroke. Effects of DHEAS in the central nervous system are mediated through GABAergic, glutamatergic and other neurotransmitter systems. The aim of this study was to investigate the effects of DHEAS on recombinant GABAA receptors by comparing the effects of this neurosteorid on human embryonic kidney (HEK) 293 cells, stably transfected and untransfected with 122S GABAA receptors, and to explore the potential neuroprotective effect of this neurosteroid using in vitro model of oxygen and glucose deprivation and reperfusion (OGDR). The study has shown that DHEAS acts as an allosteric antagonist of recombinant GABAA receptors, but that its prolonged administration does not affect the number, affinity, or the functional coupling of receptor binding sites. The results also suggested that DHEAS, applied both before and after OGDR has protective effect on HEK 293 cells, and that cytoprotective effect of this neurosteroid is at least partly achieved via GABAA receptors.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:197733
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-05-12 11:46:15